...the gRNA design process and rules for various applications is necessary going forward. The standardization... I think I may have finally grasped just how complicated DNA repair pathways are. I fully admit to being...Alt-NHEJ), has been harnessed for CRISPR knock-in applications. CRISPR 101: Targeting RNA with Cas13a (C2c2...for CRISPR Genome Editing Other resources and publications CRISPOR gRNA design tool Researchers at the ...

... to help decide whether work is suitable for publication in their journal. The early peer-review attempts...information that would prevent the study to be replicated or omission from the literature. I think that.... The code and the data can be deposited in the public on-line repository (like GitHub, Zenodo, Figshare...Addgene.org Learn about depositing plasmids pre-publication Find molecular biology protocols Find eBooks ...

... (AmpR) and origins of replication (ori and f1 ori) which allow it to replicate.   How can I prevent...the bacterial selection marker and origin of replication. During bacterial culture, only the plasmid with...the antibiotic resistance gene and origin will replicate, and the transgene will be lost. Over time, bacteria...

...under harsh conditions. One example application from the publication is the removal of unwanted cDNA derived...arrange permits to visit places not accessible to the public and retrofitting our car with a freezer wired to...a thermostable Cas9 be useful? It can enable applications where reagents must be stored at elevated temperatures...

...developmentally programmed regulation of DNA replication and gene transcription during vertebrate embryogenesis...be other scientists, or science journalists, or policy makers. The term editor covers a wide variety of...access affects financial models in information publication. Take a look at the blog ‘Scholarly Kitchen’ ...International Congress on Peer Review and Scientific Publication, where diverse approaches to peer review were...

...containing only the sequences required for viral replication and transcription as well as reporter genes. ... membranes. Read the full post for potential applications of R-bodies and to find a video of them in action...these libraries from the post and the associated publication. Optimizing Donor DNA for Enhanced CRISPR Genome... to your DNA sequence of interest. Potential applications of the system include activation and repression...

... and written communication skills are key to publication and acquisition of funding. If a grant committee...editing manuscripts? Are you interested in scientific policy? Next, use the resources below to identify potential...Understanding)   Writing Communication Publicity Consulting internships Communication...Blogging Writing Communication Networking Publicity Business competitions   Leadership ...

...antibodies may just not perform as well in one application as another, so it is always best to have options...for mEGFP-HRas (from Plasmid 18662). See the Applications section on the Anti-GFP [N86/8R] page for more...fluorescent proteins (FPs) necessary for many of these applications have lagged. Fixation, chemical staining, and...well-suited for the demands of advanced microscopy applications such as super resolution, electron, and expansion...

...protein-protein interactions. Beyond these common applications, researchers are finding new ways to apply fluorescent...Zhou and Fatima Enam. There were many excellent applicants but Xin and Fatima stood out for their creativity...utility of these FLiPs in a variety of research applications and particularly notes their ability to be precisely...Update (7/17/2018): Check out Fatima's recent publication on her HMO work in Cell Chemical Biology! Additional...

...databases available, Web of Science. For each publication, Web of Science provides basic bibliographic ...Science, PLOS One, and PNAS. We then broke the publications from these journals into two groups: those containing...differences in citations due to the journal of publication. Table 1 provides an example calculation of the...Addgene in them. The adjustment is done within publication years. What do we find once we do this adjustment...

...non-trivial, however, as FPs are tremendously complicated entities with a large range of characteristics...decision remains spread across literature in publications that introduce these tools. Years ago, Kurt...collection pages can be kept private, or shared publicly (so feel free to moderate your own version of ...incorporate FPbase data in their own projects and applications, a REST API allows programmatic retrieval of...

...for all ages. Organize your data and prepare publications or your dissertation Organize data that you...these drafts and for a few model organisms, Micropublication will accept negative data or publish brief...brief, novel findings. Most Micropublications contain one figure. If you aren’t there yet, write introductions...introductions to potential publications or prepare a review article. These are both options that can be done...

...Luciola italica)Southern Russian firefly(Luciola mingrelica) 562 64 Luciferin ATP No Jamaican click beetle...which luciferases have been cloned from include click beetles (Pyrophorus plagiophthalmus), railroad worm...

...historical standard. Specifically, in our recent publications, we used the cancer plasmid collections listed...Cancer Variants Identifies Oncogenic Alleles. Kim E, Ilic N, Shrestha Y. et al. Cancer Discovery 2016. PubMed...pathway reference collection is described in this publication We also wrote an extensive review on the exciting...data generated from these alleles in the above publications), it will be possible for many others to perform...

...Scientists can also use electroporation, the application of an electrical charge to cells, to increase... properties. The phagemids are packaged into replication-incompetent phage particles with assistance from... found on an episome, a piece of DNA that can replicate on its own or be integrated within a bacterial...Multiple phases of competence occur during the Helicobacter pylori growth cycle." FEMS microbiology letters...

...cardiovascular and metabolic diseases. We are knocking out genes that have been implicated in disease or, ...to understand the basis for cardiovascular and metabolic human diseases. They do that by studying patients...less than a month's time. Addgene: What are the implications of this kit in terms of understanding human ...

...experiments. Recombinant AAVs (rAAVs) The primary application of AAV is delivery of genetic cargo to a host...transduced gene is episomal and does not divide and replicate in the same way the host DNA does. Because of ... since you don’t have to wait for the host to replicate the DNA before expression. Now that you’ve got... S., et al. “Various AAV Serotypes and Their Applications in Gene Therapy: An Overview.” Cells, 12(5),...

...aggregates. This property can be tolerated in some applications (e.g. labeling of cells or tracking promotor...promotor activity), but it is problematic in applications in which the fluorescent protein is used as an inert...During the experiment, the solution is diluted, complicating the interpretation. Because of this, these methods...aware that the fluorescent proteins reported in publications are only characterized under a limited number...

... PMCID: PMC3918502. Find plasmids from this publication at Addgene. 2. Ma, Hanhui, et al. “Multicolor...PMCID: PMC4364232. Find the plasmids from this publication at Addgene. 3. Ma, Hanhui, et al. “Multiplexed...PMCID: PMC4864854. Find the plasmids from this publication at Addgene. 4. Kiani, Samira, et al. “Cas9 gRNA... PMCID: PMC4666719. Find plasmids from this publication at Addgene. Additional Resources on the Addgene...

...journalism.” She also interned two days a week on a public radio show. After graduating with a master’s, Susan...complete a certificate in Strategic Communications and Public Relations at the University of Washington and the...opportunities, such as writing for a student-led publication, are more readily available and it’s often easier...internship similar to Susan’s NEI internship. Application information can be found here Find tips for using...