Addgene

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Addgene's Cancer Collection Pages Connect the Research Community

Type

Blog Post

Published

Sept. 6, 2016, 2:30 p.m.

...set of vectors for use with the Gateway Cloning Platform (Life Technologies, Carlsbad, CA) that represents...
CRISPR 101: Mammalian Expression Systems and Delivery Methods

Type

Blog Post

Published

Sept. 24, 2015, 2:30 p.m.

...want to include a homology directed repair (HDR) template to create knock-ins, point mutations, or to add...
Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

Type

Blog Post

Published

May 22, 2015, 4:58 p.m.

...chromosome and its own gene is used as a repair template, thereby converting the heterozygote into a homozygote...
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

Type

Blog Post

Published

June 12, 2014, 3:30 p.m.

...Patron: Remember plants. Every time you look at your plate, think about how much that’s on it are plants, and...
Negative Can Be Positive: Open AAV Data with Addgene

Type

Blog Post

Published

Sept. 6, 2022, 1:15 p.m.

.... (2020). Methods Matter: Standard Production Platforms for Recombinant AAV Produce Chemically and Functionally...
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

Type

Blog Post

Published

Dec. 7, 2020, 2:53 p.m.

...-transposon Transform plasmid(s) into cells and plate overnight Screen colonies phenotypically or by PCR...
CRISPR 101: Ribonucleoprotein (RNP) Delivery

Type

Blog Post

Published

Sept. 6, 2018, 12:02 p.m.

... nuclear membrane, allowing for entry of a DNA template. Other techniques, such as lipid-mediated transfection...
AAVs CREATed for Gene Delivery to the CNS and PNS

Type

Blog Post

Published

Sept. 28, 2017, 2:01 p.m.

...Cre-expressing tissues. This inversion creates a template that’s suitable for PCR amplification using pre-designed...
Isolating Genomic Regions of Interest with the CRISPR System

Type

Blog Post

Published

May 30, 2019, 12:44 p.m.

...., et al. "Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression...
CRISPR 101: RNA Editing with Cas13

Type

Blog Post

Published

July 31, 2020, 12:30 p.m.

...ADAR will preferentially edit an adenine if the template has a cytosine mismatch at that position. PspCas13b-ADAR2DD...
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

Type

Blog Post

Published

July 16, 2020, 1:15 p.m.

...therapeutics. Prime editing, base editing, PACE, and DNA-templated synthesis are four examples of technologies pioneered...
Viral Vectors 101: The Retroviral Lifecycle

Type

Blog Post

Published

July 27, 2023, 1:15 p.m.

...Initiation of synthesis with 70 S viral RNA as template. Journal of Molecular Biology, 79(1), 163–183....
MXS Chaining

Type

Blog Post

Published

Feb. 7, 2017, 3:30 p.m.

...Neveu PA. MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian...
Tips for Screening with Yeast Two Hybrid Systems

Type

Blog Post

Published

Oct. 22, 2015, 2:30 p.m.

... strain for screening), or arrayed on a 96 well plate where each well contains a defined prey fusion. ...
Cpf1: A New Tool for CRISPR Genome Editing

Type

Blog Post

Published

Oct. 14, 2015, 2:30 p.m.

...confirmed. To prevent new editing post-HDR, repair templates should remove the PAM sequence. Comparing Cpf1...
Twenty Years of Sharing: Expanding Accessibility

Type

Blog Post

Published

Nov. 21, 2024, 10:47 p.m.

...through the use of popular Chinese social media platforms. As you can imagine, shipping to so many countries...
Plasmids 101: Stringent Regulation of Replication

Type

Blog Post

Published

June 20, 2024, 1:15 p.m.

...chromosomal replication. Adapted from BioRender template. Since DnaA binding is necessary to initiate...
Hot Plasmids Spring 2024

Type

Blog Post

Published

May 23, 2024, 1:15 p.m.

...called CROPseq-multi (Walton et al. 2024). This platform is compatible with enrichment, single-cell sequencing...
CRISPR-based Genome Editing Systems For Editing in E. coli

Type

Blog Post

Published

April 13, 2021, 1:15 p.m.

...targeting the genomic site of interest. The donor template is supplied separately. To use this system, you...
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

Type

Blog Post

Published

Dec. 14, 2017, 2:08 p.m.

...Blood Cells: An Efficient Protocol Using Serial Plating of Reprogrammed Cells by Centrifugation. Stem Cells...

Showing: 221 - 240 of 266 results

Results per page:

Addgene's Cancer Collection Pages Connect the Research Community

CRISPR 101: Mammalian Expression Systems and Delivery Methods

Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

Negative Can Be Positive: Open AAV Data with Addgene

INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

CRISPR 101: Ribonucleoprotein (RNP) Delivery

AAVs CREATed for Gene Delivery to the CNS and PNS

Isolating Genomic Regions of Interest with the CRISPR System

CRISPR 101: RNA Editing with Cas13

The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

Viral Vectors 101: The Retroviral Lifecycle

MXS Chaining

Tips for Screening with Yeast Two Hybrid Systems

Cpf1: A New Tool for CRISPR Genome Editing

Twenty Years of Sharing: Expanding Accessibility

Plasmids 101: Stringent Regulation of Replication

Hot Plasmids Spring 2024

CRISPR-based Genome Editing Systems For Editing in E. coli

pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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