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  1. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ...but they are laborious to generate and costly to share. What is a RANbody? A RANbody is a fusion between.... Staining with multiple HRP-RANbodies is indistinguishable since they produce the same signal, so only...

  2. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...either simultaneous or independent of the other (Shahied, 2004). They are often used for clinical applications...antib8020028. PMID: 31544834; PMCID: PMC6640713. Shahied, L., et. al. (2004). Bispecific Minibodies Targeting...

  3. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...2015; Koike-Yusa et al., 2014; Ma et al., 2015; Shalem et al., 2014; Wang et al., 2014), knock down (Gilbert... 26190106. PubMed Central PMCID: PMC4559080. 8. Shalem, Ophir, et al. "Genome-scale CRISPR-Cas9 knockout...

  4. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...One of the best things about sharing plasmids through Addgene is that we provide an added level of confidence...Serial Cloner, and Snapgene), and save these to a shared computer or server. This will ensure that future...

  6. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...stories to answer interview questions. Story telling shares many of the same elements as a STAR answer: a setting...questions can be hard during this talk since you’ll be sharing your screen and won’t be able to see who’s asking...

  7. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...competing mobile genetic elements. Image by Basem Al-Shayeb in the Doudna Lab. Scientists looking for more...your experiments? AAV-flex-taCasp3-TEVp from Nirao Shah's and Jim Well's lab induces cre-dependent apoptosis...

  9. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ... readers to more information if they desire it. Shapes. In this type of poster, written and graphical ...trickier to make figures fit nicely into circular shapes rather than a square or rectangular boxes. Background...

  10. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...help emails and phone calls, I feel compelled to share my years of accumulated wisdom so that you, the ...concern is based on data you have produced, please share the data. Maybe not all, but many bio companies ...

  11. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    Published
    Aug. 6, 2015, 2:30 p.m.
    ...Vision and Ophthalmology Research (ARVO). Hopefully sharing my experience will show you how you can get started...and benefited greatly by improving my skills and sharing with other more junior leaders what I had experienced...

  12. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...can rely on one another to celebrate good news or share a bottle of wine after a rough day, and we have ...making the switch from academia to entrepreneurship, share your thoughts with us in the comments below. We’...

  14. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...that matures quickly and the other slowly, see Verkhusha et al.), one benefit of the common FP timers is...19889833. PubMed Central PMCID: PMC2801723. 3. Verkhusha, Vladislav V., et al. "An enhanced mutant of red...

  15. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...been used for protein expression. Plant systems share many of the same features and processing requirements...loves being able to help the scientific community share plasmids.   Additional Resources Thermofisher Protein...

  16. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ... technology: because they necessarily alter the shared environment, they must never be used without popular...associated ecosystems.  Because the environment is shared by everyone, he is deeply concerned with ensuring...

  17. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...designing the best donor DNA, and avoiding common mishaps in this class of genome edit. Knock-in mutations...2020). 10.1016/j.jbiotec.2019.11.010 Wright, D. W., Shah, S. S., Heyer, W. D. Homologous recombination and...

  18. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...False, type='scriptV4', hide_playlist=True, viral_sharing=False, embed_button=False, autoplay=False, hidden_controls... on the Addgene blog Learn more about plasmid sharing during COVID-19 Resources on Addgene.org Find...

  19. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ... 1996, is an eleven β-sheet-containing “barrel” shape, with the chromophore concealed at the center of...Gal Haimovich of greenfluorescentblog. Gal will share his list of 10 things to keep in mind when choosing...

  20. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...have a shape, like a box, ellipse, or circle, drawn around clusters to define populations. This shape is ...
Showing: 221 - 240 of 490 results