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We narrowed to 279 results for: Fus

Showing: 221 - 240 of 279 results

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A Look Back at One Year of Plasmid Sharing for COVID-19 Research

Type

Blog Post

Published

July 12, 2021, 1:15 p.m.

... McLellan JS (2020) Structure-based design of prefusion-stabilized SARS-CoV-2 spikes. Science 369:1501...
Hot Plasmids and Viral Preps - January 2021

Type

Blog Post

Published

Jan. 5, 2021, 2:15 p.m.

...heterogeneous cultures. HeLa cells expressed a fusion of keratin with mGold (top) and mVenus (bottom)...
Mouse Modeling, Part 1: Genetically Engineered Mice

Type

Blog Post

Published

July 11, 2019, 1:26 p.m.

...tamoxifen-inducible Cre (Cre-ER) systems, Cre is fused to the ligand binding domain of estrogen receptor...
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

Type

Blog Post

Published

July 23, 2020, 1:20 p.m.

...spatial control of CRISPR-Cas activity. For example, fusing AcrA4II to the photo-sensitive LOV2 domain allows...
Synthetic promoter AAVs for cell-type specific expression in retinal cells

Type

Blog Post

Published

March 12, 2020, 1:15 p.m.

...transgene: an optogenetic reporter, channelrhodopsin, fused to a fluorescent GFP marker (CatCH-GFP). The lab...
Science communication: 9 Strategies to Get Your Foot in the Door

Type

Blog Post

Published

Sept. 27, 2017, 1:23 p.m.

...Sarah Schmidt is engineering resistance against Fusarium wilt disease in bananas as a Marie Curie Fellow...
Magnetic Control of Proteins: More than a Dream

Type

Blog Post

Published

Sept. 24, 2024, 1:15 p.m.

...fluorescent proteins display a similar effect (many do). Fusing EGFP to the self-labeling FlavinTag made a reliable...
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

Type

Blog Post

Published

June 25, 2024, 1:15 p.m.

... a prime editor (typically a Cas9 H840A nickase fused to a reverse transcriptase) together with a prime...
PhD Applications After COVID

Type

Blog Post

Published

Jan. 5, 2023, 2:15 p.m.

...funded? Are there any financial aspects that are confusing? During a traditional campus visit, many students...
Addgene's Cancer Collection Pages Connect the Research Community

Type

Blog Post

Published

Sept. 6, 2016, 2:30 p.m.

...different applications such as fluorescent protein fusion studies and the generation of mutant cell lines...
Google Forums Round Up: First Impressions of NgAgo

Type

Blog Post

Published

Aug. 4, 2016, 2:30 p.m.

...system indicates that it is very tricky to use and fussy to optimize. One reason could be the instability...
PITChing MMEJ as an Alternative Route for Gene Editing

Type

Blog Post

Published

Feb. 23, 2016, 3:30 p.m.

...construct is inserted into the PITCh vector via In-Fusion or SLIC cloning. Step 2: Design an insertion locus-specific...
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

Type

Blog Post

Published

July 13, 2017, 2:30 p.m.

... fly stocks and other reagents. If you’re ever confused about the best way to study YFG in flies, there...
Plasmids 101: 5 factors to help you choose the right cloning method

Type

Blog Post

Published

Aug. 21, 2018, 12:31 p.m.

...products into E. coli. Ligases within the E. coli then fuse together the PCR products via overhangs left over...
Tips to Make the Most of a Scientific Conference

Type

Blog Post

Published

May 23, 2019, 12:26 p.m.

...your elevator pitch where the other person seemed confused, bored, or particularly excited about? If you ...
No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

Type

Blog Post

Published

June 18, 2020, 1:15 p.m.

...Protein Purification RANbodies: Reporter Nanobody Fusions Plasmids 101: Secondary Nanobody Toolbox Resources...
3D Printing Meets CRISPR Cas9

Type

Blog Post

Published

Oct. 17, 2017, 12:55 p.m.

... initial model of this protein – but were very confused because the HNH nuclease active site wasn’t even...
Cloning Mammalian Cells with the Agarose Method

Type

Blog Post

Published

Sept. 7, 2017, 12:17 p.m.

...to the gene of interest (or, if your protein is fused with a fluorescent protein), fluorescence microscopy...
CRISPR 101: Mammalian Expression Systems and Delivery Methods

Type

Blog Post

Published

Sept. 24, 2015, 2:30 p.m.

... single point mutation? Do you want to create a fusion with a reporter protein such as GFP? All of these...
Antibodies 101: Producing Recombinant Antibodies

Type

Blog Post

Published

Aug. 13, 2024, 1:15 p.m.

... bioreactor supports high density HEK293 cell perfusion culture for the production of recombinant Erythropoietin...

Showing: 221 - 240 of 279 results

Results per page:

A Look Back at One Year of Plasmid Sharing for COVID-19 Research

Hot Plasmids and Viral Preps - January 2021

Mouse Modeling, Part 1: Genetically Engineered Mice

CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

Synthetic promoter AAVs for cell-type specific expression in retinal cells

Science communication: 9 Strategies to Get Your Foot in the Door

Magnetic Control of Proteins: More than a Dream

PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

PhD Applications After COVID

Addgene's Cancer Collection Pages Connect the Research Community

Google Forums Round Up: First Impressions of NgAgo

PITChing MMEJ as an Alternative Route for Gene Editing

Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

Plasmids 101: 5 factors to help you choose the right cloning method

Tips to Make the Most of a Scientific Conference

No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

3D Printing Meets CRISPR Cas9

Cloning Mammalian Cells with the Agarose Method

CRISPR 101: Mammalian Expression Systems and Delivery Methods

Antibodies 101: Producing Recombinant Antibodies

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