...more CRISPR tools are being published each month, making CRISPR a great choice for your next experiment!...control gene expression. Base editors can help you make precise point mutations near the gRNA target site...single-stranded DNA oligonucleotide, so you will need to make sure your expression system and delivery methods...Envelope plasmids provide the necessary components to make lentiviral particles. Stable, tunable expression...

...applications, and the quality of a standard curve can make or break an experiment. Here, we will provide an...the concentration of the unknown sample.  How do I make the standards for a standard curve? You create a...with BioRender.com.   When choosing a standard, make sure that the sample is pure and free of any contaminants...start with a higher concentration of standard or make smaller dilutions.        As mentioned above, it...

...messy, so you want to make sure you have sufficient volume to dip all your plants. Make sure your Agrobacterium...agrobacterium: Getting a transformant takes a long time. Make sure the agrobacterial culture you are growing definitely...increase background growth of non-transformants and make finding true transformants more difficult. Don’...

...Science: What the Future Holds and the Scientists Making It Happen, is an exercise in restraint. The very...topics without some editorializing, and he wisely makes no such attempt. He is transparent about his own...be shared by fellow scientists--but that doesn’t make his introduction to these topics any less effective... revelations. The issues he discusses have been making the rounds in journal editorials, science social...

...chemists and biochemists have developed techniques to make labeling chemistry easier and more functional in...and continual improvement of fluorescent proteins makes them the go-to choice for many (if not most) fluorescence...labeling strategies and improved fluorophores are making chemical dyes increasingly attractive and accessible...Medical Institute. He would rather be in the lab making new fluorophores for advanced imaging modalities...

...lab-use tablet? The software also makes it easy to annotate protocols, make them public for others to use,...of useful molecular cloning protocols, including making LB agar plates, restriction digest of plasmid DNA...bench, keep your inventory at your finger tips, and make shopping lists as you finish the last of a reagent...

...aggregation and cross-react with anti-GFP antibodies, making them difficult to use in a multicolor/multichannel...fluorophore-labeled antibodies with very specific reactivities making them more useful for multicolor imaging experiments...Sample preparation for these techniques sometimes makes it more difficult to detect protein tags using antibodies...Blog Rabies and neuronal tracing Retrograde AAV: Making the journey from axon to nucleus AAVs CREATEd for...

...their initial assumption. Eugene Koonin and Kira Makarova, longtime collaborators of Omar and Jonathan and.... Using computational observations, Koonin and Makarova found that Cas7-11 appeared to have derived from...generation came Prime editing — the ability to now make any small changes, including base edits, insertions...Özcan A, Krajeski R, Ioannidi E, Lee B, Gardner A, Makarova KS, Koonin EV, Abudayyeh OO, Gootenberg JS. Programmable...

...grumpiest of researchers can enjoy it at times! What makes it rewarding is when you can tell a trainee is understanding...to show up as your most prepared, can-do self and make the most of it! So, what can you do to build a ...reviewed above. Offer to help clean up and/or make necessary reagents for the experiments. Unlike in...need help with…now”. Ask for feedback so you can make adjustments. This could look like “What are some...

...pre-made viral preps. If you can’t (or don’t want to) make viral preps in your lab, there are custom viral ...verified!–your pooled library DNA, the next step is to make your virus. If the library happens to have a specific...vary with titer, cell type, and other factors, so make sure to put that aliquot to full use! Common conditions...chances that a cell doesn’t get infected at all. To make sure that every plasmid’s payload is adequately ...

...advent of CRISPR/Cas9 has made it easier than ever to make precise, targeted genome modifications. Cas9 has...many available through Addgene’s repository) and make it easy to find your perfect match. Choosing a ...xCas9 has a PAM requirement of NG, GAA, or GAT, making it a more flexible cutter with the added bonus ... You can achieve your precise edit without even making a break.  Base editors are dead Cas9 (dCas9) or...

...larger target, such as a protein. Such flexibility makes aptamers ideal candidates for biosensors. Aptamer... of how they actually “report” as biosensors. Making aptamers glow The flexibility of aptamers means...rather than separate them (Fig. 1b). One can also make “signal-off” biosensors in which the aptamers fluoresce...decrease background in vivo. The nature of aptamers makes them excellent candidates for developing assays ...

...nowadays, we cannot make it a full month without a new, drug-resistant ‘superbug’ making headlines in one... develop survival mechanisms. Some microbes even make their own antibiotic compounds. In such a case, ...resistant bacteria to over-take the population and making them more difficult to treat in the future. Patients...

... for talk is over and instead we must make real changes to make the system sustainable. For years, there...Department of Labor issued a Notice of Proposed Rulemaking, soliciting feedback by Sept 4th.  They proposed...opportunity to accept these recommendations, and make gradual adjustments towards this new level. Indeed...

...preference, some people like to make stricter gates while some like to make looser ones (Figure 3). A more...free to use SSC for a single cell gate. However, make sure to compare H and A (or W and A).   Live... type of stain whether a tighter or looser gate makes sense. Here, luckily, the vast majority of my cells...

...yielding 1-10% knock-in. Read on to learn how you can make CRISPR mouse model generation easi-er! Generating...months. But certain models remain more difficult to make. For example, large insertions, like a fluorescent... site. If you are using the ivTRT method, try to make the first two bases of the left homology arm guanine...activity. To avoid re-cutting by Cas9 after editing, make sure that the repair template does not contain the...

...used for delivering your light stimulus. This can make or break your experiments!  First, closely read ....2021.778900 Mattis, J., Tye, K. M., Ferenczi, E. A., Ramakrishnan, C., O’Shea, D. J., Prakash, R., Gunaydin, L... B., Kadmon, J., Raja, C., Chibukhchyan, A., Ramakrishnan, C., Inoue, M., Shane, J. C., McKnight, D. J...science.aaw5202 Mattis, J., Tye, K. M., Ferenczi, E. A., Ramakrishnan, C., O’Shea, D. J., Prakash, R., Gunaydin, L...

... of DNA as inert. It generally stays put, which makes it easy to locate in a genome. But there is a type...genomes of many other prokaryotes and eukaryotes, and make up ~45% of the human genome (Munoz-Lopez et al.,...considered replicative since every time they jump they make a copy of themselves. This increases the number ... by their nature, are mutagenic elements, which makes them a great tool for mutagenesis screens that detect...

...you get the idea. What if there were a way to just make one fusion protein that switches to any color or...experiment (Figure 3). And using self-labeling tags makes updating your labels easy! Instead of taking the...ligands is more expensive than letting the cells make a fluorescent protein for you. And some ligands ...Selecting Fluorescent Dyes and Ligands GCE4All: Making Genetic Code Expansion Accessible Addgene's Genetic...

...non-dividing cells, including stem cells. These properties make lentiviral vectors fantastic options for delivering...such as macrophages and microglia. This property makes the lentivirus an attractive choice for a gene transfer...the accessory factors in HIV positive cells could make the virus replication competent. The 3rd generation...transgene resulting in low inducibility and potentially making it difficult to interpret your results. This can...