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Showing: 201 - 220 of 499 results

  1. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...American Type Culture Collection and freeze as many early passage vials as possible. One problem that often...can make this assumption incorrect. One of the earliest steps in lentiviral transduction is the interaction...

  2. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ... determine this is to examine expression of the early response gene c-fos. For example, using Chr2 stimulation...and a lot of room for error, optogenetics is not nearly as difficult as it seems. Like every other advanced...

  3. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...CRISPR Cas variant that can also cleave dsDNA similarly to Cas9 (Zetsche et al., 2015 ). Cas12a however...DETECTR. How does DETECTR work? DETECTR works similarly to SHERLOCK. Cas12a is targeted to a specific ...

  5. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ... Janelia, are now available! Building on their earlier Voltron indicators (a fusion of Ace2N and HaloTag...Neurodegeneration Research Collection. This page is regularly updated with useful materials for research into...

  6. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...What is an aptamer? Nearly 30 years ago, two independent groups, led by Jack Szostak and Larry Gold, ... Assays that require recognition of ions or particularly small molecules that do not generate an immune...

  7. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...article is an interactive chart where you can see the yearly interdisciplinarity index of research for single...and case study." Journal of Librarianship and Scholarly Communication 2.2 (2014): 2. Additional Resources...

  8. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    Published
    Oct. 25, 2016, 2:30 p.m.
    ...major effect on scientists within academia, most particularly postdocs working in the U.S., whose current ...thing. Reliance on a cheap trainee workforce has clearly not been a sustainable approach to biomedical research...

  9. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...the introduction of this mutation can be any site early in the coding region of the gene of interest. This...Repressing target genes using dCas9-based repressors Early experiments using dCas9 in bacteria demonstrated...

  10. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ... to keep up with the latest tools. In Addgene’s early days, we would ‘spot-check’ critical regions of ...us to keep pace with our current need to analyze nearly 300 sequences each week. Our internal software ...

  11. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...mouse model generation simpler, as described in an earlier post on generating CRISPR mouse models. For simple...the example at right using blue flame plasmid pmScarlet_C1! After creating the T7-DNA template, you'd use...

  13. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ... your technical knowledge and judgement. For particularly tricky cases, team discussions can often lead...doing a lot of science communication, and is particularly excited to hear about novel developments in ...

  17. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...between cell types or organisms, promoters must be similarly variable. Bacterial promoters only work in prokaryotic...related species from which they were derived. Similarly, the various eukaryotic cell types (mammalian,...

  18. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...while requiring a lower concentration of sample — nearly ten times less protein. While the addition of LEAs...Methyltransferase (CHARM), with several advantages over the earlier system.  Rather than overexpressing an exogenous...

  19. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...this way, if you know what you need. Most often, early career scientists aren’t trained to have these types...based on financial values. All too often, we see early investigators making unnecessary short term sacrifices...

  20. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...distancing. With countries like South Korea, one of the earlier epicenters of the outbreak ultimately flattening... is a one-pot, one-temperature reaction, and is nearly single-molecule sensitive. In the case of HIV-1...
Showing: 201 - 220 of 499 results