...When this interaction occurs, BirA* activity is restored, and nearby proteins are labeled with biotin. ...but labeling ceases when these two bait proteins stop interacting. This feature is particularly useful...

...plasmids at Addgene! Knock-in: Introducing tags or stop signals Once Cas9 generates double-strand breaks...very long ncRNAs is impractical. Instead, tags or stop elements can be inserted to disrupt the transcription... adjacent genes are complex and not yet fully understood, so it is important to carefully select the target...

... In the US, UC Santa Cruz, MIT, UW Madison, and Boston University offer science communication programs... and money to a full-time graduate course, UWE Bristol offers an intensive 4-day Masterclass in science...or upload your data to Zooniverse. The Natural History Museum has downloadable resources to enable you...

...so many scientists who were potential Addgene requestors and depositors. China is in one of the top 10...good job of seeing tRNAs because they contain “hard stop” mutations. New techniques used by the Lowe lab ...can view public circRNA datasets and download the custom python scripts needed to discover circRNAs in RNA-seq...

...Women’s Hospital/Harvard Medical School and then Boston University (institutions where I’ve worked as a...became interim Chair automatically as it was an understood expectation of any Vice-Chair. Following an official...Juliet Moncaster is an Assistant Professor at the Boston University School of Medicine and is the former...

...methods. First, its modular design allows for customization with strain-specific promoters, antibiotic resistance...species where its modular nature allows for the customization of guides, promoters, selection markers, and...(2005): 443. PubMed PMID: 15908923. Johnson, Christopher M., and Alan D. Grossman. "Integrative and conjugative...

... inner cell mass (ICM) of a 5-6 day old human blastocyst [6]. They are isolated mostly from abnormal and...PMCID: PMC2752493. 4. Bongso, A. and M. Richards, History and perspective of stem cell research. Best Pract...Derivation of embryonic stem-cell lines from human blastocysts. N Engl J Med, 2004. 350(13): p. 1353-6. PubMed...

...approach gives me a bit of flexibility since I can store the cell lysates at -80 °C for up to 6 months before... completed in one long day, or the virus can be stored at 4 °C and buffer exchanged the next day. Refer... comments below! Special thanks for Dr. Dan Stone and Harshana de Silva Feelixge at Fred Hutchinson...

...human genes and are recommended for use in immunohistochemistry (Figure 5). Anti-MAPT [AT8] specifically...Addgene Antibody Data Hub.  Figure 5: Immunohistochemistry with Addgene's Anti-APP [6E10-A5] recombinant..., Wang, W. (2024). Anti-APP [6E10-A5] in Immunohistochemistry (Human). Addgene Report, doi: https://doi.org...

...course, you will want to make sure that your final stocks only contain rAAV. To minimize contamination, use...preparation.  rAAVs  Now your AAV virions are ready to store or use. They are quite stable and will withstand...Londono, L., Arjona, K., Parianos, M., Truong, V., Rostonics, E., Hernandez, A., Boye, S. L., Boye, S. E., ...

...spectral flow and can be thought of as a series of histograms flipped onto its side and compressed into a heat...for rare cellular markers, beads can sometimes distort the fluorescent signature upon antibody binding...: Secondary AntibodiesAntibodies 101: Buffers, Storage, and Conjugates Resources on Addgene.org Addgene's...

...for a scientist. I think that establishing this history helped demonstrate that a) I was a real person ...which demonstrated to the students that scientific history was very much in the making. One of the exciting... in an exciting, tumultuous time in scientific history. Whatever your reasons, let us know how it goes...

...using and whether you are inoculating from a frozen stock or a colony from an LB plate. In my hands, a 5 mL...culture can be used for colony PCR and glycerol stocks. Dip/drip plants more than once: Dip your plants...University. She studies cell fate maintenance in the stomatal lineage and has made countless transgenic plants...

...biology also falls within this section. The GFP story has its own Franklin in Doug Prasher, who originally...describes the detection of gravitational waves--a story I was much less familiar with as a biologist--as... feel slightly dated by next week.   A stepping stone When I discussed The State of Science with the five...

....io beta platform for sharing, annotating, and storing life science protocols. Since we began sharing ...management and you want the works: organizing reagent storage and inventory, managing orders, tracking students... scientists workflow in mind and can offer a one-stop solution to your lab's organization and management...

...college I studied development and evolution of histone genes in Strongylocentrotus purpuratus, the spiny...Discovering ways to culture, propagate, transform, store, and share all of these new organisms continues ...promiscuous and the gene that is responsible is understood. It’s possible to actually silence this gene ...

...vaguely resemble a bagpipe and historically, bagpipes were made using the stomachs of different animals. Check...originated from. Let’s take a look at some of the stories behind the names. We also have many plasmids in...

...frustrating it can be to get a “bad batch”); Customizable sensitivity and detection - since they frequently...changes after target binding, aptamers are highly customizable in terms of how they actually “report” as biosensors...a higher melting temperature than its Spinach ancestors, and has a lower magnesium dependence. Though ...

... Commons license.  Histograms  For analysis of a single marker, a histogram showing frequency of events... “true” signal.  Figure 8: A series of histograms from Alyamani et al., 2018, showing number of...

...like the cold or flu) Overuse of antibiotics in livestock farming Lengthy courses of treatment with antibiotics...those bacteria that are partially resistant. If you stop a treatment early, you may be un-wittingly selecting...used in the clinical laboratory. What can we do to stop the emergence of antibiotic resistance?  The problem...