...of similar interests and goals. The following questions can serve as a guide when selecting a research...impact on your field? You want to choose a research question that people (especially you!) care about. ...project along the way. Advice from principal investigators Tom Ellis highlights the importance of keeping... apart a piece that can address a topic in a mechanistic way using the technology available.” Choosing... your project will be fresh as well.” Don't underestimate the importance of choosing a solid research ...Posts in this Blog Series Our 3 PIs answer more questions, including: Advice for Choosing a Research Project...

...Massachusetts Institute of Technology, Boston University, Johns Hopkins University, the Salk Institute, and the...USA-Europe not only provide the specific technical and logistic support to increase the chances of success, but...evidence of this, you needn’t look any further than testimonials from the students who participated in the program...common problems that many others have faced yet, still, this challenge did not hinder them from advancing... the Karolinska Institutet, among others). Mentors and mentees held scheduled monthly progress video conferences...universities and high level public and private institutions also showed their support and hope for the IMP...

...Leiwe and Takeshi Imai from Kyushu University. Stochastic multicolor labeling is a powerful solution for...microscopy-based neuronal reconstruction. To achieve stochastic multicolor labeling, Brainbow used the Cre-loxP...copies of the transgene cassette are introduced, stochasticity will result in a combinatorial expression of...Brainbow was inherently low. This is because the stochastic and combinatorial expression of fluorescent proteins... such vector-based gene delivery systems, the stochastic expression of XFPs is possible by simply introducing...introducing a mixture of XFP genes. Stochastic multicolor labeling is possible when each of the XFP genes...

...sharing on the site. The entry form has 5 general question types and it takes approximately 15 minutes or... have all of your data prepared. All of these questions relate to factors that you should have already...re running your next AAV experiment, use these questions as a guide for designing your future injection...information so we can follow up with you if we have any questions and so that we can give you recognition for sharing... strain. Experimental design & methods: These questions are focused on injection and detection methods...data. Please note that the data hub is currently still in beta version, so we’re always looking for ways...

...came as an undergrad at Worcester Polytechnic Institute. Though some WPI students chose to go the solo...goal, or at the very least, a clear reason for existing. Here are a few types of teams that exist at Addgene... team A Process Team is a group of people with distinct skills who fulfill a long-term function. When ...biochemist, a geneticist, a microscopist, a biostatistician, and that one golden-handed technician who ...responsibilities are. That’s a subtle but important distinction. If I invite someone to be on a project team,...out the discussion themselves, then one has to question whether that person should be on the team. As ...self-interest ahead of the team’s goals, then that team is destined to do poorly. To some extent, it doesn’t even ...

...students are confronted with the question ‘what now?’ This is often a hard question to answer if you plan on ...These skills show employers that you can fully investigate and solve a problem or complete a project, overcoming...Manager Patent Lawyer Science Market Research Statistician   Attention to detail Patience, accuracy ...including: Science Reporter Technical Writer Statistician Bioinformatician Science Writer Grant Writer...like for someone who currently in the position. Questions you’ll want to find answers to include: Will ...

...Daley, Christine Mummery, Lorenz Studer, and Deepak Srivastava--addressed a battery of questions regarding...particularly in fields like stem cell biology where questions of ethics can have profound impacts on the development...banked iPSCs across international borders. While questions of ethics can be daunting because they have no...cell community for addressing these important questions that the scientific method cannot address. Listen...already mentioned, Addgene depositors Marius Wernig, Kristin Baldwin, Kathrin Plath, Hans Schöler, and closing...

...don’t get the same result. So, then they end up investing time that they thought would be moving forward... build on it going forward. This is not just a question of being honest and straightforward, it’s an important...do we get where we want to be? The part that is still missing is a clear alignment of incentives. Right... in science needs both a job and funding, so institutions and funders, to a large extent, hold the keys...is clearly room to do more. If we could really instill a culture of rewarding good scientific citizenship...UK and then a postdoc at The Scripps Research Institute in La Jolla, California. She joined Elsevier after...

...study 1: Cell lines Many of the reagents used to investigate SARS-CoV-2 were developed for broad use and are.... Seventeen of these cell lines were used to investigate the susceptibility of cells from different organisms... Bioresources Cell Bank, part of the National Institutes of Biomedical Innovation, Health, and Nutrition...choose the best experimental model for further investigation of this pathogen. The sharing and distribution...The Jackson Laboratory, a nonprofit research institution, has been able to rapidly propagate this strain... rapid pace of SARS-CoV-2 research by removing logistic hurdles to continue making materials available...

...upon who your challenger was, you might have to drastically alter your strategy. I realized that similar ...As your students play, you can observe, answer questions, and guide them to productive strategies while...they had 10 minutes to expose their strategy, justifying each single element used to build the construct...concepts required by the course and some were so enthusiastic that they took the cards and the board with ...modifying BUILD YOUR PLASMID You can find rules, suggestions, and more details on how to play the game on ...biomedical education for future generations. All suggestions, tips and critiques to improve the educational...

...repair bias is a fascinating (and yet unanswered) question. Our recent paper (Richardson et al 2016) revealed...that process. We began our research with a simple question: how do Cas9 and sgRNA interact with and dissociate...from a DNA target? The surprising answer to this question led us to develop a new, rational approach to ...followed these experiments with more detailed investigations into the release of DNA post-cut. Using substrate...repair that normally accompanies Cas9 cutting. We still don’t know the mechanism underlying dCas9 editing...Postdoctoral Researcher in Jacob Corn’s lab investigating DNA repair pathways.     Additonal Corn Lab ...

...life of its own. Nevertheless, there are still many open questions to be clarified, especially with regard... field, often referred to as 'frugal science', existing components are adapted and combined with creativity...raw data (CARE), segmentation of imaging data (ILASTIK), or the popular plugin-based image process framework... put their work on the shoulders of giants. The still relatively young movement of sharing open hardware...References Joel T. Collins, Joe Knapper, Julian Stirling, et. al, "Robotic microscopy for everyone: the...

...we can’t forget that those other components are still there in real life.  Certainly referring to any ...Chromosome Organization. These aren’t the only investigators in the chromatin field who have deposited with...associated PI pages on the Addgene website. In-exhaustive List of Addgene Depositors Who Study Chromatin...Chromatin Cheryl Arrowsmith James Bradner Kerstin Bystricky Courtney Hodges Sharon Dent Archa Fox Elaine...consider searching directly on our website. In-exhaustive Chromatin-related genes with plasmids on Addgene.org...@theReddyLab), Thoru Pederson, Joanna Wysocka, Kerstin Bystricky, Ben Martin, and Berkley Gryder. Addgene...

... the DNA, or enzymatic digestion. Sonication provides a truly random digestion, but is harsher and requires...requires more optimization. Enzymatic digestion, while gentler on the sample, introduces bias due to the...sonication is usually the preferred method, enzymatic digestion can be appropriate when your protein of interest...cofactor. In such cases, the gentler enzymatic digestion can preserve more of the protein:DNA complexes...After crosslinking and sonication or enzymatic digestion, you’re ready to incubate the sample with your...

...Have you ever tried digesting with XbaI or ClaI restriction enzymes and gotten unusual or unexpected ...site-directed mutagenesis experiment? The answer to both questions is the same--methylation! Read on to learn about...from the restriction modification systems, yet can still affect whether certain restriction enzymes can effectively...potential for methylation should be considered when digesting DNA. Why? Well, even though Dam methylation sites...performing a digest for cloning purposes or for diagnostics, we suggest double checking to make sure your...affected by methylation. This table is by no means exhaustive, so you may want to consult the REBASE database...

...toxicity, it would be advantageous to instead use a preexisting, conserved cellular machinery to process gRNAs...any 5’ leading nucleotide could be processed, a distinct advantage over other methods that favor certain...indicating that Cas9 can be guided to at least 8 distinct loci. They also examined editing efficiency in...multiplexing in systems with limited packaging capacity. Still confused about the various tRNA expression strategies...systems available from Addgene. If you have any questions or thoughts about this technology, leave them ...them in the comments below.  References Boosting CRISPR/Cas9 multiplex editing capability with the endogenous...

...synthetic biology is still in its infancy. A long timeline from transformation to testing and a lack of well-characterized... of plants are being investigated on limited funding and time. While institutions that focus on creative...problem solving like the Howard Hughes Medical Institute have begun to pivot towards plant biotechnology...Biophysics and Biochemistry from the Illinois Institute of Technology in 2004, she accepted a commission...

...varying degrees of β-arrestin-2 recruitment to receptors, indicating their agonistic nature. The potency...microscopy techniques, such as Stimulated Emission Depletion (STED), and Stochastic Optical Reconstruction Microscopy...help. Here, we will outline some fundamental characteristics to consider when selecting a dye or ligand ...Fluorescence microscopy takes advantage of the characteristics of these fluorophores to enable highly specific...non-fluorescent states in response to a specific stimuli, which leaves only a small volume of fluorophores...

...appropriate containers. The machine is useful for distinguishing cell populations that have been tagged with...visualize how cells interact after a particular stimulus. Using an epifluorescence microscope and GFP fusion...easily track GFP expressing cells. For example, by harvesting lymph nodes at different times after antigen ...immunologists the ability to count cells and statistically analyze cell movements. By using cells labelled...immune response, but the study of these dynamics was still laborious as epifluorescence microscopy wasn’t able...aureus and eating the bacteria. Some of the many questions scientists have been able to help answer using...understood thanks to these technologies but many questions remain. The use of other technologies such as ...

...stranded RNA (ssRNA), while some exist as dsRNA. Still others use dsDNA to store their genetic information... made it into agriculture as species-specific pesticides and insecticides, an advancement over previous...sequence. All of the R-loops described above play distinct roles in promoting cellular processes and functions...While not perfect, these sensing systems work to distinguish between RNA of cellular vs. viral origin and ... each one acting on specific substrates and in distinct cell cycles. When RNA:DNA hybrids levels are allowed...appreciate double stranded isn’t just for DNA. Untwisting RNA is important for therapeutic delivery, viruses...