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  1. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ... biological system of interest, the so-called “fishing expedition.” Your first question is: to screen ...library resources or our RNAi resources and go fishing! References and resources References Wang, T.,...
  2. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ...long distances. When it comes to breast cancer, Hoshinho and colleagues found that after injecting breast...cell 21.3 (2012): 309-322. PubMed PMID: 22439926. Hoshino, Ayuko, et al. "Tumour exosome integrins determine...
  3. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ...systematically and logically is as useful for finishing your thesis as it is for helping your team reach... organization Time management Managing a team Leadership Resources on Addgene.org Check out our careers...
  4. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ...the appropriate stab culture Grow the stab culture Ship the stab culture to the proper location To keep...technicians to retrieve the sample and get it ready for shipping. In the first case, we naively assume that everything...
  5. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...focus of our first decade. We did this the old-fashioned way: through physical outreach, visiting organizations... our repository grew to over 150,000 plasmids, shipped out our two millionth item, and had a cumulative...
  6. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ... research environment” with a strong focus on publishing, but still made it so she could “get exposure...work. She says, “I spend a lot of time packing, shipping, and sharing protocols one by one,” and she is...
  7. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ... editing while still allowing on-target editing (Shin et al., 2017). Acrs could also be used to reduce....e9 . https://doi.org/10.1016/j.cell.2016.11.017 Shin J, Jiang F, Liu J-J, Bray NL, Rauch BJ, Baik SH,...
  8. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...Poster layout While it’s good to let your ingenuity shine, and you generally have free reign over how you ...catchy, but beware. A distracting background or clashing colors can take away from what should be the focus...
  9. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...upstream of the PAM site, Cpf1 cleaves in a staggered fashion, creating a 5 nucleotide 5’ overhang 18-23 bases...publication at Addgene. 4. Makarova KS, Wolf YI, Alkhnbashi OS, Costa F, Shah SA, Saunders SJ, Barrangou ...
  10. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...cell populations.  Acknowledgement:Through a partnership with genOway, we are able to distribute materials...Luo Massimo Scanziani Scott Sternson Roger Tsien Naoshige Uchida Ian Wickersham Hongkui Zeng Additional...
  11. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ...categorization is complex Plasmid classification has shifted over the years, and new classifications are still...organization doesn’t necessarily reflect genetic relationships or plasmid incompatibility. Plasmids may also...
  12. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...strong signal. However, exposure time and its relationship to signal depend on what you’re comparing your...working with Dr. Wu please contact us here! Ava Shipman is a research assistant in lab of Zhuhao Wu at ...
  13. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ... science.  Prepare the night before: To avoid rushing out the door without your poster, place it by your... you may receive. People often ask about the relationship of your work to recently published papers or...
Showing: 201 - 220 of 454 results