Skip to main content
Addgene
Showing: 201 - 220 of 343 results

  1. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...://doi.org/10.7554/elife.06100 Liu, L. S., Zhao, L. Y., Wang, S. H., & Jiang, J. P. (2016). Research proceedings...

  2. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...iRFP713, % Oligomeric state Photo-stabilityc, t1/2, s pKa Brightness in HeLa cells vs. iRFP713, % d Ref...239 (2020). Oliinyk, O.S., Shemetov, A.A., Pletnev, S., Shcherbakova, D.M. & Verkhusha, V.V. Smallest near-infrared...

  3. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...orthogonal nuclease-inactive Cas9's (dCas9) from S. pyogenes, N. meningitidis and S. thermophilus which have been...favorite cells. Ma et al., Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112...620nm) portions of the spectrum. PhD student Benjamin S. Padman recently developed a mitochondrially-targeted...context. Chatterjee A et al. Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):11803-8. Plasmid tools for...

  4. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    .... Liu XS, Wu H, Ji X, Stelzer Y, Wu X, Czauderna S, Shu J, Dadon D, Young RA, Jaenisch R (2016) Editing...plasmids from this publication at Addgene. Stolzenburg S, Beltran AS, Swift-Scanlan T, Rivenbark AG, Rashwan...

  5. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...topics. Resources and references References Aydin S (2015) A short history, principles, and types of ELISA...10.1016/0003-2697(81)90281-5 Pillai-Kastoori L, Heaton S, Shiflett SD, Roberts AC, Solache A, Schutz-Geschwender...

  6. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...like Antibodypedia or through your antibody provider(s) if they accept and share user data on the antibody...10.1016/j.nbt.2018.08.002  Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, Rodriguez...

  7. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...lately (1, 2), depends on knowledge of the structure(s) a given phage will recognize on a host cell. To ..., Callum J., Mohammadali Khan Mirzaei, and Anders S. Nilsson. "Adapting drug approval pathways for bacteriophage-based...

  8. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...as effective at editing mouse embryos as Cas9 from S. pyogenes and has the advantage of being smaller (...health.     References Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini...

  12. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ...photoconvertible proteins. With Sattarzadeh et al.’s discovery that GFP itself is photoconvertible, new...

  18. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ...10.1038/cr.2009.139 Janda, A., Bowen, A., Greenspan, N. S., & Casadevall, A. (2016). Ig Constant Region Effects...

  19. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    Published
    May 4, 2023, 1 p.m.
    ...parental cell line and the X knockout with your drug(s). If a drug is equally toxic to both lines, it probably...
Showing: 201 - 220 of 343 results