... direct ELISA, aside from the detection step. Similarly, the protein antigen is coated onto the wells ...calculate the protein concentration. Image from Jagarlamudi et al., 2015.   The maximum OD that most ...

...could be repurposed for SARS-CoV-2 RNA detection. Early in the pandemic, labs around the world have been...  Expression of SARS-CoV-2 viral proteins In the early weeks of the COVID-19 pandemic, the Krogan lab at...

...Why study neural connectivity? One of the early lessons many of us learned in biology is that the body...Deshpande et al., 2013). The study found that at early time points, most of the projections to these new...

... to prevent dCas9 tethering to DNA. Since these early anti-CRISPR studies, scientists have discovered ...can increase the chance of off-target editing particularly after the majority of on-target editing has ...

...seen some major advances in light microscopy, particularly related to the neurosciences.  These developments...tetrahydrofuran-based tissue clearing (13,14). A few years later, Karl Deisseroth’s lab developed CLARITY, which is a hydrogel-based...

...cytometry (“spectral flow”) has emerged as a particularly useful tool for those wishing to run larger ...Mayo Clinic studying host-microbe interactions in early life.    Resources More resources on the Addgene...

...expression cassette in one single viral particle. The earliest explorations of the use of AdV for in vivo CRISPR-Cas...of AdV-modified human cells being subjected to scarless homology-directed genome editing (Maggio et al...

...experience, the more I heard that getting into industry early would only help. Like any major life decision, ...could be laid off while you’re there. Of course, similarly, an academic advisor could also lose their funding...

... close to the hood so you can view your cells regularly (both before entering the hood and when putting...Institute of Colloids and Interfaces. Sana is particularly interested in Glycobiology and Immunology. You...

...dsDNA in most starting materials, and is not particularly sensitive. The fluorescent signal can be affected...be affected by DNA quality and primer design, particularly for amplicons under 100 bp (Ma, 2015.) A schematic...

...xCas9 3.7. Base editor variants with xCas9 are similarly flexible. Like the xCas9 3.7 nuclease, xCas9 3.7... and base editing, to more loci. Although these early data are exciting, there’s a lot we still don’t ...

...creates chimeric circles of DNA that are distant linearly but close together in space. The chromatin “picture...enhancer is interacting with. Super enhancers are particularly good models for building chromatin interaction...

...The maturation of the chromophore on many FPs (particularly those derived from GFP) requires oxygen. Therefore...interested in everything related to gene expression, particularly at the RNA level. He maintains the greenfluorescentblog.wordpress.com...

...Lentiviral vector history and components In the early 1990s, researchers developed viral vector systems... but this research is still in the pre-clinical/early clinical stages. Researchers are also working to...

...didn’t attend. For example, we got a good bit of early feedback from those who wanted, but were unable ...carefully organized. Most importantly, they should be regularly assessed for continued relevance and if needed...

...experiences. STEM in elementary schools - catching them early I’m fortunate to live in a town with a good public...means it was the best kind of networking! Since nearly every talk I’ve given in the past 20 years has ...

...conjugate, and the complexity of the system. A particularly desirable property for small-molecule labeling...removing excess dye from the sample, which is particularly important for samples where washing is difficult...

...information while using less resources and are particularly useful when trying to screen a limited number...discriminate between non-essential and essential genes similarly to the Brunello knock-out library while mitigating...

...mere millimeters apart. Similarly, cells compact DNA in a way that brings linearly distant regions close...

... is still a problem today. It is now known that nearly 500 cell lines suffer from contamination with HeLa...out on low passage cells and this means you can regularly discard the cells you are working with because...