...purpose: fast growth, high-throughput cloning, routine cloning, cloning unstable DNA, preparing unmethylated... Hte   High transformation efficiency deoR constitutive expression of genes for deoxyribose synthesis...disruption of arabinose metabolism pathway Inability to utilize arabinose as a carbon source leuB β-isopropyl...XL1 Blue Tetracycline Blue/white screening and routine cloning, nalidixic acid resistant. endA1 gyrA96...

... the University of California, San Diego. The revolution in genetic engineering techniques is a speculation...the noteworthy work of fiction that it inspired. CAUTION: Spoilers alert! Pre-DNA Era  H.G. Wells, the...negative. But these negative depictions serve as cautionary tales for the entire scientific community and...Trading Card seen in this blog post?Download high resolution versions here.  Many thanks to our guest blogger...

...generation is safer than the first one and can be used routinely in a research laboratory. The risk of generating...used to express your gene of interest either constitutively or inducibly. If your gene of interest is toxic...cells - even your own! You should therefore be cautious about inserting potentially harmful genes into...information and always be sure to consult your institution’s Biosafety Committee before beginning any work...

...perspectives, the optimism they encapsulate, and the solutions they promote help motivate you to spend a little...from celebrities falsely claiming vaccines cause autism to politicians saying that climate change is a ...skepticism to promote harmful policies that benefit polluting industries.In March, three climate scientists ...citizens first and scientists second and that it is our duty to tell our science stories in a manner that encourages...communities at Harvard University to studying an evolutionary arms race at University of California, Berkeley...

...preparation for our upcoming affinity reagent distribution launch - and we're excited to say our long naming...to help us with our upcoming affinity reagent distribution launch! There was only one problem - we didn't... biggest discovery yet: Addgene will soon be distributing affinity reagents. Name Our New Antibody Friend...

...academic institutions can afford to hire. It will be difficult for some of these institutions to manage... Increased Postdoc Wages,” Future of Research executive director, Gary McDowell, gives a thorough yet ...

... room above), but as Joanne Kamens, Addgene’s executive director, said during our celebratory lunch, it...’ve enjoyed seeing scientists sharing their contributions on social media as well the excitement amongst...community. For example, we started a new service distributing ready-to-use viral preps in 2016 and we just...

..., is a repository for monoclonal antibodies, distributing over 3,000 antibodies and associated hybridoma...1986, to enable basic research by banking and distributing monoclonal antibodies at cost. We distribute...the University of Iowa. The minimal fees from distribution funds our operation. Antibodies are glycoproteins...

...directly collaborates with NIH-funded researchers and utilizes grants to support our own initiatives, Addgene...Addgene is uniquely positioned to help research institutions maximize their increasingly limited resources...investment isn't lost. Universities and research institutions can turn to Addgene as a trusted partner for...

...plasmids to more than 90,000 scientists at 5,200 institutions in 82 different countries. We continue to ship...beginnings in 2004 to today, you can see how plasmid distribution has grown. Additionally, more and more scientists...plasmids. The partnerships and collaborations with institutions and tech transfer offices that facilitate MTAs...

...are more than welcome in contributing to The Addictive Brain. A contribution can either be an article,...Brain to spread awareness about how scientists utilize tax-payer money and government funding for research...

...optical quality (magnification of 140X and 2 micron resolution). I have worked many hours in a dark room with...microscope. Less fluorescence admittedly, but still so beautiful! Enabling the scientist in everyone - everywhere...costs, deep tissue penetration, and high spatial resolution are benefits of this technique. It is even possible...

...more sustainable products Recycling is great for rerouting waste from the landfill, but what about all the...do. Remember, it was only 50 years ago that we routinely dumped toxic waste into the oceans, a practice...continue to examine our behavior and innovate solutions and practices to meet higher environmental standards...

... the lab. Whether you’re tired of calculating dilutions or you can’t quite remember the hazards of that..., Lianna Swanson, and researcher at Sanofi Pharmaceuticals. This post highlights a rare disease, Fibrodysplasia...and has huge potential for the development of therapeutics. It also represents a first for Addgene - while...

.../Cas9 effector (LACE) system, the Gersbach lab utilized a similar strategy to develop an optimized photoactivatable...repair (HDR). This system is unique in that it utilizes a “split” nuclease - the authors fragmented Cas9...the figure below. Their most successful design utilized Magnet photoswitchable proteins derived from the...far-red light-activated split-Cas9 (FAST) system constitutively expresses the C-terminal fragment of Cas9 while...

... data. Here are some tips to help you in your execution:  Accuracy is not as important as precision for...need to run a validation assay in which serial dilutions for the target and housekeeping genes are run ...plotted against the log input concentration for each dilution. For the ΔΔCT method to be accurate, the absolute...Palais R.; Wittwer CT. uMELT: prediction of high-resolution melting curves and dynamic melting profiles of...

...impressive 1,350 scientists from more than 275 institutions all over the world. In the course of all those...community and contribute in a way that everyone could utilize what we had made,” Baird said. “It won’t sit in...scientific community," says Joanne Kamens, Addgene's Executive Director, "and to promote the great work of Michael...

...signal-to-background ratio, thus, allowing higher resolution in samples containing substantial autofluorescence...ultrasound waves as opposed to light to produce higher resolution images in vivo. “One of the reasons we can’t ... less. As a result, ultrasound enables higher resolution images at depths up to eight millimeters – not...

...second clean up step to remove the cleaved tag from solution. MBP antibodies are available for detection and...purification, be aware that the tag dimerizes in solution, which can affect downstream applications. Also...called polyhistidine tags, typically include six consecutive His residues (or more). Histidine bonds with ...

...fulfill our mission by generating revenue through distribution of research materials. Our small shipping and...generate revenue (as Addgene does through materials distribution). In fact, healthy nonprofits should have some...request plasmids (via web interface, processes, distribution, and customer service) Addgene provides unmatched...