...them.   When you think about it, virtually every milestone in a scientist's career is mediated by some kind...flagship workshop.  Our 2018 workshop took place in Boston - you can revisit the activities of our attendees...careers in science communication The strength of story telling Science communication: 9 strategies to get...

...RNA-editing Cas13 enzymes have taken the CRISPR world by storm. Like RNA interference, these enzymes can knock ...from this paper are available at Addgene. Yan, Winston X., et al. “Cas13d Is a Compact RNA-Targeting Type...Plasmids Check out our CRISPR Guide Read our CRISPR History  ...

...technology or as a therapeutic.  A (very) brief history GCE came onto the synthetic biology scene in 1989.... While the codon used is often one of the three stop codons ­– most commonly the UAG codon – in some ... V. W. (2021). Genetic Code Expansion: A Brief History and Perspective. Biochemistry, 60(46), 3455–3469...

... addition, there was an entire story-telling session organized by Story Collider and which featured a ...the different parts of the brain stimulated by stories. In retrospect, I should have expected this given...

...assembled a cross-team team, with members from the Customer Service, Data Curation, Outreach, Product, Scientist...particular and our deep understanding of Addgene’s customer’s needs. Categories We created a categorization...of our information in order to more granularly customize the search engine, and a scoping mechanism that...

...in C++ and Java Command line history: Tracking command line history by recording all commands entered...addition to output files, this is also where you can store any power point, excel, or other files relevant ...

...project, I read the book “Every Other Thursday: Stories and Strategies from Successful Women Scientists... group to be intrinsic to their success. Their stories made it clear to me that, executed correctly, the...renowned Healthcare Businesswomen’s Association (HBA) Boston Chapter and the Massachusetts Association for Women...

...well received, and then when the team started brainstorming ideas for the next workshop, they realized that...to practice and teach reproducibility An early milestone for the group was the “reproducibility for everyone... Schwessinger for helpful conversations on the history of the group, lessons learned, and to Sonali Roy...

...targeted the following antigens: GFP, RFP,  the histone protein H2A2B, and the actin-binding protein gelsolin...however, compatible with most multi-labeling immunohistochemical protocols where either a directly labeled...RANbodies) as versatile, small, sensitive immunohistochemical reagents. PNAS. PubMed PMID: 29440485. Additional...

...six figures. Averaging ten panels each. (For this story, it is important to know that the student presenting... it. When my mind went blank, my options were to stop talking until I could present something coherent... something as simple as telling yourself, “I can stop and check my notes if I need to,” or “It is okay...

...CRISPR editing in human embryos has been met with a storm of media coverage. But the paper published August... studies, the edited embryos proceeded to the blastocyst stage at similar rates to untreated controls....researchers were able to examine each individual blastomere to determine its genotype. However, this detailed...

... by guest bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular Cytology and Van ...our guest bloggers Joachim Goedhart and Marieke Mastop! Joachim Goedhart is an assistant professor at ...follow him on twitter: @joachimgoedhart.   Marieke Mastop is a PhD candidate at the Section of Molecular ...

...Segall-Shapiro, Thomas H., Eduardo D. Sontag, and Christopher A. Voigt. "Engineered promoters enable constant...2. Kittleson, Joshua T., Sherine Cheung, and JChristopher Anderson. "Rapid optimization of gene dosage...Synthetic Biology: A Conversation with MIT’s Christopher Voigt Resources on Addgene.org See the synthetic...

...faculty (at time of writing, Brigham And Women's in Boston also just announced that they were reversing their...does not appear to hold. There are institutions in Boston - Massachusetts General Hospital, Mass Eye and ...that are not raising salaries when all others in Boston (some of them literally across the street from ...

...indicated below and pool-clone them into the pAWp28 storage vector (Figure 1): Forward oligo: 5’- CACCGNNNNNNNNNNNNNNNNNNNGTTTGGGTCTTCGAGAAGACCTATTCXXXXXXXXC...bp barcode, respectively. Once cloned into the storage vector, the pooled, barcoded gRNA library is now...backbone pAWp12 (Figure 2). Cloning from the pAWp28 storage vector to the pAWp12 lentiviral vector should retain...

...part of the recognition sequence, they can be customized to direct assembly of DNA fragments. When designed...utilize the BsaI and BsmBI Type IIS sites such that custom TAL arrays could be constructed quickly and efficiently...Nov 5. PubMed. Efficient design and assembly of custom TALEN and other TAL effector-based constructs for...

...experiences. Good coaches will have loads of success stories and plenty of people to vouch for them. Trust... of scientists here) I know many coaches in the Boston area. Sarah Cardozo Duncan is a proven coach who...has an outstanding blog and, while her PhD is in History, I think she would be a good match as a coach for...

...science, dating back several hundred years, with historical ties to psychology and philosophy (the mind-body...in a certain type of behavior (for a review see Stoica et al., 2013). Vector delivery may also be achieved...service for their behavioral studies. References Stoica L, Ahmed SS, Gao G, Sena-Esteves M. Gene transfer...

...your target protein in each well. The reaction is stopped by the addition of hydrochloric acid (for more ...(TMB). The addition of hydrochloric acid, which stops the reaction, turns the solution yellow. The absorbance...serial dilution of your experimental sample, and/or stopping the HRP-TMB reaction more quickly. Just make sure...

...born before 1985, you might remember going to the store and buying CDs when you wanted to hear a piece of...technology makes it easy for scientists to share, remix, store, and track these essential components of the scientific...useful in perpetuity.  Second, plasmids are easy to store and share, making recombinant antibodies the most...