... peptide-based proteosome inhibitors, glutathione S-transferase (GST), which can be fused to recombinant...Purification Very small size, rarely affects function S-tag KETAAAKFERQHMDS  1.8 Solubility and Affinity Abundance...

... then the MoClo YTK Kit specifically designed for S. cerevisiae is at your service (Lee et al., 2015)....al., 2017). In addition to additional elements for S. cerevisiae chromosomal integration and gene expression...//doi.org/10.1021/acssynbio.9b00322. Kim, Matthew S., et al. Degron-Based bioPROTACs for Controlling Signaling...

...precise deletion products compared to monomeric Cas9’s. Read the paper in Nature Communications Find ...

...Matho, K. S., Cetin, A., Woodard, K., Cepko, C., Guerin, K. I., Rego, M. A., Ersing, I., Bachle, S. M., Kamens...

...nucleotides of DNA can serve as the target for Cas9’s single guide RNA (sgRNA), provided it meets two conditions...system uses a “nicking” Cas9, in which one of Cas9’s two critical enzymatic residues has been converted...

...2412-2420. PubMed PMID: 25101481. Filonov, Grigory S., et al. "Broccoli: rapid selection of an RNA mimic...Central PMCID: PMC4923196. Panchapakesan, Shanker Shyam S., et al. "Ribonucleoprotein purification and characterization...

...References and resources References Im K, Mareninov S, Diaz MFP, Yong WH (2018) An Introduction to Performing...fluorescence-microscopy/   Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, Rodriguez...

...Ferreira DM, Krook A, Barres R, Zierath JR, Erhardt S, Lindskog M, Ruas JL. doi: 10.1016/j.cell.2014.07.051...

... transposase. The donor plasmid contains the gene(s) of interest flanked by terminal repeat sequences,...They contain only a eukaryotic promoter and the cDNA(s) to be expressed and they don’t integrate or replicate...from this reference. 1. Malik, Nasir, and Mahendra S. Rao. "A Review of the Methods for Human iPSC Derivation...

...involves making specific knock-out edits in a gene(s) of interest and has an average off-target frequency...CRISPR experiment? CRISPR 101: Cas9 The Other Cas(s) CRISPR 101: Validating Your Genome Edit         ...

...iRFP713, % Oligomeric state Photo-stabilityc, t1/2, s pKa Brightness in HeLa cells vs. iRFP713, % d Ref...239 (2020). Oliinyk, O.S., Shemetov, A.A., Pletnev, S., Shcherbakova, D.M. & Verkhusha, V.V. Smallest near-infrared...

...://doi.org/10.7554/elife.06100 Liu, L. S., Zhao, L. Y., Wang, S. H., & Jiang, J. P. (2016). Research proceedings...

.... Young, Carissa L., Zachary T. Britton, and Anne S. Robinson. "Recombinant protein expression and purification...

...lately (1, 2), depends on knowledge of the structure(s) a given phage will recognize on a host cell. To ..., Callum J., Mohammadali Khan Mirzaei, and Anders S. Nilsson. "Adapting drug approval pathways for bacteriophage-based...

.... Liu XS, Wu H, Ji X, Stelzer Y, Wu X, Czauderna S, Shu J, Dadon D, Young RA, Jaenisch R (2016) Editing...plasmids from this publication at Addgene. Stolzenburg S, Beltran AS, Swift-Scanlan T, Rivenbark AG, Rashwan...

...like Antibodypedia or through your antibody provider(s) if they accept and share user data on the antibody...10.1016/j.nbt.2018.08.002  Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, Rodriguez...

...topics. Resources and references References Aydin S (2015) A short history, principles, and types of ELISA...10.1016/0003-2697(81)90281-5 Pillai-Kastoori L, Heaton S, Shiflett SD, Roberts AC, Solache A, Schutz-Geschwender...

...as effective at editing mouse embryos as Cas9 from S. pyogenes and has the advantage of being smaller (...health.     References Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini...

...orthogonal nuclease-inactive Cas9's (dCas9) from S. pyogenes, N. meningitidis and S. thermophilus which have been...favorite cells. Ma et al., Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112...620nm) portions of the spectrum. PhD student Benjamin S. Padman recently developed a mitochondrially-targeted...context. Chatterjee A et al. Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):11803-8. Plasmid tools for...

..., Addgene has “literally enabled sharing with 100's of investigators worldwide. Now everything from every...