...CRISPR effector protein they named Cas7-11. Testing showed that Cas7-11 cut RNA with unprecedented specificity...luciferase RNA knockdown assays using Cas7-11   Use The Cas7-11 targeting system contains two pieces: ...both of which we're featuring in today's blog. Cas7-11 Development and Discovery As part of their quest ...looked at the transformed E. coli expressing Cas7-11, they found no evidence of growth inhibition when...negative effects in RNA targeting experiments. If Cas7-11 lacked this toxicity, it would represent a major ...  The team originally suspected that this new Cas7-11 might be phylogenetically classified as the more ...observations, Koonin and Makarova found that Cas7-11 appeared to have derived from the more complex type...

...are split between strand 10 and 11 to yield large (1-10) and small (11) fragments. In several cases an... FP(11) fragments, even from a different base FP (for example, EBFP2(1-10) can be used with GFP(11)). ... Figure 1: Two proteins are fused to the FP(11) and FP(1-10) fragments. When Protein A and B interact...EBFP2 EBFP2(1-10) and Capri(1-10) for use with GFP(11) Multiplexed labeling of cellular proteins with split...Kamiyama Cerulean Cerulean(1-10) for use with GFP(11) Multiplexed labeling of cellular proteins with split...-folder GFP, our most-requested GFP(1-10) and GFP(11) Versatile protein tagging in cells with split fluorescent...Brian McNaughton mNeonGreen2 mNG2(1-10) and mNG2(11) Improved split fluorescent proteins for endogenous...

...doing rotations? 8:01 - 11:25 : How did you decide what lab to join? 11:25 - 11:50 : What is the number...rotation students should ask senior graduate students? 11:51 - 13:28 : How do you define what a good mentor...What's been your biggest experimental failure? 26:11 - 31: 32 : What's been your biggest experimental ...

...FlipGFP, sfCherry was split into  β1-9 and β10-11, where β10-11 was forced into a parallel conformation by...different self-assembling split GFP (GFP1-9 and GFP10-11) by flipping a single β-strand of GFP, hence the ...et al., 2019). How does FlipGFP work? GFP contains 11 β-strands and one central ɑ-helix that can be split... ɑ-helix, (2) β-strand 10 (β10), and (3) β-strand 11 (β11). Each part contains components and residues...was dependent on protease activation. Normally, β10-11 are antiparallel. To prevent binding, the β strands...thus can no longer fit with β1-9. They connected β10-11 with two linkers (E5 and K5) that dimerize together...

...Best for: targeting single stranded RNA.   Cas7-11 Cas7-11 is a naturally occurring RNase composed of the...between Cas7-11 and Cas13 are 1). non-specific RNA cleavage and 2). cell toxicity. Cas7-11 does not display...the fusion of several Cas7 and Cas11 domains. Cas7-11 cuts ssRNA and is capable of pre-crRNA self-processing... of collateral cleavage). You can read about Cas7-11 in our blog post on its development and use. Fast...targeting with the single-protein CRISPR effector Cas7-11. Nature, 597, 720-725 (2021). 10.1038/s41586-021-...

...give their insights as to what causes this dip. 11:11 - 15:37 : How have you changed throughout graduate....com/watch?v=lQGAsgjjBDI&feature=youtu.be. 8:17 - 11:10 : The 3rd year of graduate school and what makes...

..., a bright red pigment reporter for plants.  Cas7-11 and PASTE: Omar Abudayyeh and Jonathan Gootenberg...worked with us to share their new CRISPR tools, Cas7-11, a low-toxicity RNA editing tool, and PASTE, a drag-and-drop...

... the proportion of virulent S. aureus from 50% to 11% in just 24 hours.   Figure 1: Single-phage...efficiently-delivered RNA-guided nucleases.” Nature Biotechnol. 32(11): 1141-5. (2014). PubMed: PMID 252409288. PubMed ...sequence-specific antimicrobials.” Nature Biotechnol. 32(11): 1146-50. (2014). PubMed: PMID 25282355. PubMed ... next-generation antimicrobials.” Genome Biol. 15(11):516. (2014). PubMed: PMID 25417800. PubMed Central...

...10:30-11:41 : Kingdom on the difficulties surrouding and need for biotech research in Malawi 11:41-14:...

...listen! Podcast breakdown (Time : Topic) 0:00 - 7:11 : Introduction - Ghanaian scientist's molecular biology...the Addgene, Seeding Labs plasmid grant. 7:12 - 7:11 : Kwabena's work in Christopher E. French's Lab at...

... iPS cells without antibiotic selection. Nat Meth 11, 291–293 (2014). PubMed PMID: 24509632. PubMed Central...Specific Screening of Genome Edited Cells. PLoS ONE 11, e0153901–17 (2016). PubMed PMID: 27089539. PubMed...-dPCR) mediated by designer nucleases. Nat Protoc 11, 598–615 (2016). PubMed PMID: 26914317. 4. Mock, ...

...layers, form embryoid bodies, and form teratomas [11]. iPSCs also possess similar morphologies, growth...properties, and express stem cell markers similar to ESCs [11].  While iPSCs and ESCs have similar functional utility...2007. 318(5858): p. 1917-20. PubMed PMID: 18029452. 11. Medvedev, S.P., A.I. Shevchenko, and S.M. Zakian...

...substrates containing gaps or DNA base pair mismatches (8-11). For some applications, there cannot be any end-... near the ligation junction with high efficiency (11, 21). Ligases are thought to interrogate dsDNA for...thermostable DNA ligase-encoding gene." Gene 109.1 (1991): 1-11. PubMed PMID: 1756968. 7. Lauer, G., et al. "Cloning...PubMed PMID: 3684572. PubMed Central PMCID: PMC306403. 11. Wu, Dan Y., and R. Bruce Wallace. "Specificity of...

...generation as does replacing C-MYC with L-MYC [10, 11]. While the mechanisms underlying the enhancement...non-integrating episomal vectors. PLoS One, 2011. 6(11): p. e27956. PubMed PMID: 22132178. PubMed Central...PMID: 19668191. PubMed Central PMCID: PMC2917235. 11. Nakagawa, M., et al., Promotion of direct reprogramming...

...Originally published Oct 11, 2016 and last updated Dec 22, 2020 by Benoit Giquel. CRISPR has taken the...plants enabled by a biorecognition motif. Nat Commun 11: . https://doi.org/10.1038/s41467-020-15731-w Wang... ERF Transcription Factor Gene OsERF922. PLoS ONE 11:e0154027 . https://doi.org/10.1371/journal.pone.0154027...

...Additional human IgG1s are also available on demand. The 11 IPI synthetic rAbs, developed using yeast display... integrins, the RGD-binding integrins. Six of the 11 IPI antibodies contain RGD-mimetic sequences that...binding specificity, kinetics, and affinity of these 11 newly discovered synthetic rAbs. We then used these...

... mRNA with 90-99% knockdown efficiency across the 11 candidate transcript targets analyzed. CRISPR-Csm...via focused ultrasound. Nat Biomed Eng. 2021 Nov;5(11):1336-1347. doi: 10.1038/s41551-021-00779-w. Epub...

...Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. Epub 2014 Oct 11. PubMed. Find the plasmids...

...keratinocytes. Nat Biotechnol, 2008. 26(11): p. 1276-84. PubMed PMID: 18931654. 11. Loh, Y.H., et al., Reprogramming...generation of human cardiomyocytes. Nat Methods, 2014. 11(8): p. 855-60. PubMed PMID: 24930130. PubMed Central...induced pluripotent stem cells. Stem Cell Rev, 2015. 11(2): p. 242-53. PubMed PMID: 25578634. PubMed Central...

...the OpenFlexure microscope," Biomed. Opt. Express 11, 2447-2460 (2020) Hongquan Li, Deepak Krishnamurthy...open standard for microscopic imaging. Nat Commun 11, 5979 (2020). https://doi.org/10.1038/s41467-020-...