...mTurquoise2 Dorus Gadella 98816 3xnls-mScarlet-I Nucleus 3xNLS mScarlet-I Dorus Gadella 98876 4xmts-mNeonGreen...mTurquoise2 Dorus Gadella 98818 4xmts-mScarlet-I Mitochondria 4xmts mScarlet-I Dorus Gadella 19031 pABCb10-GFP...* Michael Davidson 98831 mScarlet-I-LaminB Nuclear Envelope LaminB mScarlet-I Dorus Gadella 98830 mTurquoise2...Gadella 98821 Lck-mScarlet-I Plasma Membrane Lck N-terminal lipidation signal mScarlet-I Dorus Gadella 50057...mTurquoise2 Dorus Gadella 98827 Lamp1-mScarlet-I Lysosomes Lamp1 mScarlet-I Dorus Gadella 55073 mCherry-Lysosomes...LifeAct mScarlet-H Dorus Gadella 85056 pLifeAct_mScarlet-i_N1 Actin Filaments LifeAct mScarlet-I Dorus ... Gadella 85053 pmScarlet-i_H2A_C1 Nucleus H2A mScarlet-I Dorus Gadella 85052 pmScarlet-H_H2A_C1 Nucleus...

...characterized: mScarlet (fluorescent lifetime of 3.9 ns, quantum yield of 0.70), mScarlet-I with T74I (accelerated...compared to mScarlet, fluorescent lifetime of 3.1 ns, quantum yield of 0.54) and mScarlet-H with M164H...compared to mScarlet, fluorescent lifetime of 1.3 ns, quantum yield of 0.20). All three mScarlet variants...New bright monomeric red fluorescent protein - mScarlet! The Dorus Gadella lab has recently developed ...synthetic red fluorescent protein variant they call mScarlet. Bindels et al used a combination of deliberate...protein fusions. The Gadella lab has deposited all mScarlet constructs with Addgene. Bindels DS, et al. Nat...Methods. 2017. PubMed PMID: 27869816 Listen to our mScarlet Podcast Segment    Photocleavable protein PhoCl...

... other hand, mScarlet-I shows correct ER labeling, which is good evidence that mScarlet-I acts as a monomer...the Addgene Blog Which Fluorescent Protein Should I Use? Choosing Your Fluorescent Proteins for Multicolor...

...Mammalian Expression pCS2+mScarlet-C Cloning Vector - Mammalian Expression mScarlet-I 569 593 57 5.4 36 min...Expression mScarlet-I3 568 592 68 4.2 2 min Monomer pmScarlet-I3_C1 - Mammalian Expression mScarlet3 569 592...Monomer pmScarlet3_C1 - Mammalian Expression mScarlet 569 594 71 5.3 2.9 hr Monomer pmScarlet_C1 - Mammalian...Maturation Structure Plasmids mScarlet-H 551 592 15 4.8 4.4 hr Monomer pmScarlet-H_C1 - Mammalian Expression...Michael Davidson Collection Blog: Which FP Should I Use? Blog: How to Choose a FP For Multi-Color Imaging...min Monomer pmScarlet-i_C1 - Mammalian Expression mStrawberry 574 596 26 4.5 50 min Monomer mStrawberry-N1...

...cells include: mTurquoise2, mEGFP, mNeonGreen, mScarlet(-I), and mCherry. Keep the linker sequence short...instance, Lifeact-mTurquoise2 (Plasmid #36201) or mScarlet-tubulin (Plasmid #85045). We often use restriction...protein of interest can be challenging. In this blog I discuss strategies to generate fusion proteins and...interest and the fluorescent protein. These were either (i) coded by the multiple cloning site and therefore ...often use terminology like “C-terminal fusion,” but I find this very confusing because it can mean that ... is at the C-terminus of the fluorescent protein. I prefer to describe the different parts of the fusion...

...ShadowR is used as a FRET acceptor to mRuby2 or mScarlet. Image: Murakoshi et al., 2019. ShadowR is ... a FRET acceptor in combination with mRuby2 or mScarlet as the donor in HeLa cells.   Find ShadowR for...bf00428249 Chan MCY, Karasawa S, Mizuno H, Bosanac I, Ho D, Privé GG, Miyawaki A, Ikura M (2006) Structural...

...proteins that had shown magnetic responses: EGFP, mScarlet, and AsLOV2. After several rounds of semi-random...semi-random mutagenesis and screening, the EGFP and mScarlet variants were showing no obvious signs of improved...Evans, E. W., Déjean, V., Dodson, C. A., Wallace, M. I., Mackenzie, S. R., Timmel, C. R., & Hore, P. J. (...

... of an array of different reporters including mScarlet-I, luciferase, antibiotic resistance markers, and... Figure 4: Reporters in Gene On include a mScarlet reporter, luciferase reporter, and neomycin resistance...

...Fon-bREACHes-EYFP Flp AND NOT Cre 137158 pAAV-nEF-ChRmine-mScarlet None 137159 pAAV-nEF-Con/Fon-ChRmine-oScarlet ...Zalocusky KA, Bernstein H, Swanson H, Perry C, Diester I, Boyce FM, Bass CE, Neve R, Huang ZJ, Deisseroth K...Regulation. Neuron 101(1):45-59. PMID:30554781 Lazaridis I, Tzortzi O, Weglage M, Märtin A, Xuan Y, Parent M,...

...fusion for expression in plants, co-expressed with mScarlet-I for ratiometric imaging David Nelson 141301 pRATIO1267...increased usefulness in in vivo applications. LuxSit-i : An artificial luciferase desined de novo using deep-learning... in vivo . DULIP ( DU al L uminescence-based C o- I mmunoprecipitation) plasmids : Luminescence-based ...

...markers tagged with mTurquoise2, mNeonGreen, and mScarlet-I for labeling a list of specific structures within...

...the use of bright red fluorescent proteins (e.g. mScarlet) or infrared fluorescent proteins (Chernov et ...factors contribute to the popularity of GFP including (i) fast and complete maturation to functional, fluorescent...discussed below. There are numerous GFP variants. Here I use the name ‘GFP’, which refers to mEGFP. This variant...

...pScarlet-Kif5a KIF5A mScarlet CMV ALS Beverly Koller 118745 pCAG-Scarlet-Kif5a KIF5A mScarlet CAG ALS Beverly...Arrowsmith 184825 pSN837 KIF5A mScarlet CMV ALS Kyoko Chiba 184826 pSN838 KIF5A mScarlet CMV ALS Kyoko Chiba 184827...184827 pSN526 KIF5A mScarlet Strep polH ALS Kyoko Chiba 184832 pSN840 KIF5A mScarlet Strep polH ALS Kyoko...Spinocerebellar ataxia James Trimmer 190693 pSN879 KIF5A mScarlet CMV ALS Shinsuke Niwa 190803 FUGW-APPwt-T2A-mCherry...CMV, P10 Huntington's Cheryl Arrowsmith 112205 pBS-I-Sce1-eno2:Tau-IRES-egfp MAPT GFP, eno2 eno2 Parkinson's...Spinocerebellar Ataxia Richard Youle 176464 pVPS13D-I-EGFP VPS13D GFP CMV Spinocerebellar Ataxia Richard...

...animals than i-GONAD and is a more technically difficult process. Overall, the main drawback of i-GONAD is...responsible for Easi-CRISPR have also recently developed i-GONAD as a simple method for delivery of genome editing...germline. In contrast to standard microinjection, i-GONAD is performed directly on pregnant female mice...Ohtsuka et al. compared traditional microinjection and i-GONAD knock-in efficiency using a small ssDNA, and... with a long ssDNA donor was less efficient using i-GONAD (15%) vs. microinjection delivery (25-67%). ....2017.153 PubMed PMID: 29266098 Ohtsuka, Masato, et al. i-GONAD: a robust method for in situ germline genome...the example at right using blue flame plasmid pmScarlet_C1! After creating the T7-DNA template, you'd use...