...promoter of lac and trp Regulated like the lac promoter Contains -35 region from trpB and -10 region from...crossover. Generally speaking, promoters in bacteria are less diverse and complex, having fewer parts than those...Cauliflower Mosaic Virus Constitutive  Active in dicots, less active in monocots, with some activity in animal...Constitutive  Murine U6 is also used, but may be less efficient.   Prokaryotic promoters     Promoter...expression through varied inducer concentrations. trp High levels of gene expression Promoter from E. coli...Vectors Read about Reporter Gene like Luciferase and GFP Resources on Addgene.org Find Plasmids for Your...Constitutive  Tends to give consistent expression regardless of cell type or physiology. SV40 General expression...

...Church lab uses this system to control expression of GFP.)  To turn this inducible system into a repressible...Researchers have engineered pADH1 variants that are less sensitive to ethanol, including the “middle” ADH1...the fungus Neurospora crassa. The QUAS promoter is less leaky than UAS in the basal state, and co-expression...tac promoter: a functional hybrid derived from the trp and lac promoters.” Proc Natl Acad Sci U S A. 80(...

...48201 CAG-GFP-IRES-CRE Cre and GFP coexpression CAG Retroviral Gage 49054 CAG-GFP/cre Cre-GFP fusion CAG...116879 CAG-Cremyc-2A-GFP GFP and Cre CAG Mammalian Lu 117148 Hiv7CMV-Cremyc-2A-GFP GFP and Cre CMV Lentiviral...iRFP670-EFS:Cre-2A-GFP iRFP670, Cre, and GFP TRE Mammalian Jacks 68544 AAV-Cre-GFP Cre CMV AAV Nestler...13770 pCALNL-GFP Cre dependent GFP expression Mammalian Cepko 8389 p212 pCMV-EGFP/RFP EGFP-dsRed gene switch...promoterless CRE-GFP fusion none Mammalian Sauer 11960 pBS537 tet-hCMV-GFPcre tet inducible Cre-GFP fusion tet-hCMV... CMV Lentiviral Fuchs 26646 pCAG-Cre-IRES2-GFP Cre and GFP coexpression CAG Mammalian Chenn 26647 pCAG-Cre...CAG Retroviral Gage 49056 AAV-GFP/Cre Cre-GFP fusion CMV AAV Gage 49111 pEMS1980 iCre with MCS for inserting...

...selectivity for cell surface labeling may become less selective if used in intracellular labeling because...the cell lines with lower expression should have less antibodies labeling them. We find that cell tracker...measurable through an epitope tag such as FLAG/His/GFP. Fluorescent protein tags, which correlate closely...myofibroblasts to cell death via down-regulation of TRPA1 channels. British Journal of Pharmacology, 178(15... antibodies for the specific detection of human TRPA1. Scientific Reports, 9(1), 18500. https://doi.org...

...pPS808 - N-terminal GFP for yeast expression pET GFP - C-terminal GFP for...gene expression GFP Varies pBABE GFP - Retroviral gene expression pMKO.1 GFP - Retroviral ...C elegans expression GFP Localization pcDNA3-EGFP - C-terminal GFP for mammalian expression... from the SP6 promoter pCS2 mt-GFP - Create myc-tag, GFP-tag fusions for transcription ...expression pcDNA3 GFP LIC cloning vector - C-terminal GFP for mammalian expression...expression MSCV-IRES-GFP - Mammalian retroviral gene expression with GFP selectable ...cassette pBTG (pBigT-IRES-GFP) - pBigT modified to contain IRES-GFP Hynes Lab ...

..., Hess et al. successfully evolved wild type GFP to EGFP using CRISPR-X and subsequent FACS sorting. They...with unique tissue-specific promoters expressing GFP, tetracycline response elements, and shRNAs many ... of DsRed connected to a pH-sensitive variant of GFP (SEP) by a 9 amino-acid linker (see Figure 1). The...selective targeting of fusion proteins, requires less biotin, and shows enhanced labeling of proximate...The applications for such a modular system are endless and, as scientists use GMAP, there will be a continuous...ferritin protein were tethered to the C-terminus of TRPV4 (a pressure-sensitive channel). The Guler lab optimized...Plasmid ID Plasmid Name Plasmid Type 74309 pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry Mammalian Expression ...

...contain a reporter gene (for example, luciferase or GFP) that offers a read-out of the activity of the genetic...AGC Threonine Thr T ACU, ACC, ACA, ACG Tryptophan Trp W UGG Tyrosine Tyr Y UAU, UAC Valine Val V GUU, GUC... constructed a plasmid, you can easily make an endless number of copies of the plasmid using bacteria,.... Visit our viral service page to learn more. Regardless of type, plasmids are generally propagated, selected...Antibiotics Create a stock solution of your antibiotic. Unless otherwise indicated, the antibiotic powder can ...