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We narrowed to 7 results for: Trp-less GFP

Showing: 1 - 7 of 7 results

  1. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...promoter of lac and trp Regulated like the lac promoter Contains -35 region from trpB and -10 region from...crossover. Generally speaking, promoters in bacteria are less diverse and complex, having fewer parts than those...Cauliflower Mosaic Virus Constitutive  Active in dicots, less active in monocots, with some activity in animal...Constitutive  Murine U6 is also used, but may be less efficient.   Prokaryotic promoters     Promoter...expression through varied inducer concentrations. trp High levels of gene expression Promoter from E. coli...Vectors Read about Reporter Gene like Luciferase and GFP Resources on Addgene.org Find Plasmids for Your...Constitutive  Tends to give consistent expression regardless of cell type or physiology. SV40 General expression...

  2. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...Church lab uses this system to control expression of GFP.)  To turn this inducible system into a repressible...Researchers have engineered pADH1 variants that are less sensitive to ethanol, including the “middle” ADH1...the fungus Neurospora crassa. The QUAS promoter is less leaky than UAS in the basal state, and co-expression...tac promoter: a functional hybrid derived from the trp and lac promoters.” Proc Natl Acad Sci U S A. 80(...

  3. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...expression vector GFP Varies pMKO.1 GFP - Retroviral shRNA expression pLenti CMV GFP DEST - ...Fluorescent proteins (GFP, mCherry, etc) Localization pcDNA3-EGFP - C-terminal GFP for mammalian expression...genome MSCV-IRES-GFP or pMSCV-IRES-mCherry FP - Mammalian retroviral gene expression with GFP or mCherry selectable...Inducible Expression page Bacteria Lac, T7, araBAD, trp mTagBFP2-pBAD - Protein expression vector with C-...fusing your protein to another protein, such as GFP, which allows you to visualize the cellular localization... cells, integrate into host genome pLenti CMV GFP DEST - Lentiviral Gateway destination vector for ...PGKpuro2ABFP - For retroviral delivery of one sgRNA pMKO.1 GFP - Retroviral shRNA expression Retroviral backbones...

  4. Cre-lox system

    Type
    Collection
    ...48201 CAG-GFP-IRES-CRE Cre and GFP coexpression CAG Retroviral Gage 49054 CAG-GFP/cre Cre-GFP fusion CAG...116879 CAG-Cremyc-2A-GFP GFP and Cre CAG Mammalian Lu 117148 Hiv7CMV-Cremyc-2A-GFP GFP and Cre CMV Lentiviral...iRFP670-EFS:Cre-2A-GFP iRFP670, Cre, and GFP TRE Mammalian Jacks 68544 AAV-Cre-GFP Cre CMV AAV Nestler...13770 pCALNL-GFP Cre dependent GFP expression Mammalian Cepko 8389 p212 pCMV-EGFP/RFP EGFP-dsRed gene switch...promoterless CRE-GFP fusion none Mammalian Sauer 11960 pBS537 tet-hCMV-GFPcre tet inducible Cre-GFP fusion tet-hCMV... CMV Lentiviral Fuchs 26646 pCAG-Cre-IRES2-GFP Cre and GFP coexpression CAG Mammalian Chenn 26647 pCAG-Cre...CAG Retroviral Gage 49056 AAV-GFP/Cre Cre-GFP fusion CMV AAV Gage 49111 pEMS1980 iCre with MCS for inserting...

  5. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...selectivity for cell surface labeling may become less selective if used in intracellular labeling because...the cell lines with lower expression should have less antibodies labeling them. We find that cell tracker...measurable through an epitope tag such as FLAG/His/GFP. Fluorescent protein tags, which correlate closely...myofibroblasts to cell death via down-regulation of TRPA1 channels. British Journal of Pharmacology, 178(15... antibodies for the specific detection of human TRPA1. Scientific Reports, 9(1), 18500. https://doi.org...

  6. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ..., Hess et al. successfully evolved wild type GFP to EGFP using CRISPR-X and subsequent FACS sorting. They...with unique tissue-specific promoters expressing GFP, tetracycline response elements, and shRNAs many ... of DsRed connected to a pH-sensitive variant of GFP (SEP) by a 9 amino-acid linker (see Figure 1). The...selective targeting of fusion proteins, requires less biotin, and shows enhanced labeling of proximate...The applications for such a modular system are endless and, as scientists use GMAP, there will be a continuous...ferritin protein were tethered to the C-terminus of TRPV4 (a pressure-sensitive channel). The Guler lab optimized...Plasmid ID Plasmid Name Plasmid Type 74309 pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry Mammalian Expression ...

  7. Molecular Biology Reference

    Type
    Guide
    ...contain a reporter gene (for example, luciferase or GFP) that offers a read-out of the activity of the genetic...AGC Threonine Thr T ACU, ACC, ACA, ACG Tryptophan Trp W UGG Tyrosine Tyr Y UAU, UAC Valine Val V GUU, GUC... constructed a plasmid, you can easily make an endless number of copies of the plasmid using bacteria,.... Visit our viral service page to learn more. Regardless of type, plasmids are generally propagated, selected...Antibiotics Create a stock solution of your antibiotic. Unless otherwise indicated, the antibiotic powder can ...
Showing: 1 - 7 of 7 results