...candidate at the University of Cambridge. One of the most important steps in the CRISPR experimental process... a variety of options for CRISPR genotyping. The most common options include mismatch cleavage assays,...genome and sgRNA. This is a good starting point for most researchers as it provides a list of putative off-target...effects in the clinic. NGS-based methods provide the most complete information profile regarding putative ...comprehensive list of off-target editing events. Two of the most common cell-based methods are genome-wide, unbiased...using just genome-wide unbiased methods. However, most investigators use single cell clones for in vitro...

... job opportunities in academia - the main reason most people enter postdoctoral training. Similarly, there...example). This blog post is about how to get the most out of your postdoctoral training experience. If... biomedical postdoc fellowship lasts five years. Most universities and institutes annually advertise for...you how to write as well as mentor. Yet, even the most dedicated mentor is not always available for mentees... you or the audience. Ask yourself: What is the most exciting story I can tell with my data?  Can I make...this blog add up to a substantial amount of time- most of your waking hours. This is doable and it's a ...

...your experiments, I've described below some of the most frequently requested vectors in our repository and... consider as you make your choice. The first and most important thing you need to know is your expression...or a fusion protein. Mammalian vectors One of our most popular mammalian expression vectors, c-Flag pcDNA3... YGOI in an isolated, controlled environment. In most cases bacterial expression vectors are inducible... We have a variety of other types of vectors for most applications used to study gene function. From making...

...replicate the plasmid in addition to its own chromosomal DNA, costing additional resources to maintain... have also been used to disrupt genes at the chromosomal level. Scientists introduce an antibiotic resistance...Tips and tricks from the bench Use fresh stocks. Most antibiotics are stable in powder form, but quickly...and avoiding repeated freeze/thaw cycles will keep most antibiotics viable for at least 6 months. Ampicillin...Ampicillin and can be used in place of Ampicillin in most applications. Antibiotics vary in their sensitivitly...

...unusual ploidy and are considered hypotriploid: most chromosomes are represented by two to three copies, but...but several have four. No Y chromosome is present in this line, making it genetically female. HEKs are...suspension. Of the many derivatives of HEK-293, the most commonly used line expresses SV40 large T antigen...transcriptome studies of the cell line have determined it is most likely adrenal in origin. (Anatomy refresher: the...

...which is the primary distinction between two of the most frequently used yeast strains: S. pombe (fission...pombe or S. cerevisiae. Actually, it’s neither. The most commonly used yeast by biotech and pharmaceutical...Facultative anaerobe Obligate aerobe Number of chromosomes 16 3 4 Preferred carbon source Glucose...media buck - it grows at higher cell densities than most of its counterparts, yielding higher production ...lacks certain terminal mannose glycopeptides (which most other yeast possess) that are problematic for human...

...students. I believe that communication is the single most important skill that scientists need to succeed ... that you can succeed in communicating. Even the most rigorous, insightful, and novel scientific research... skill in science communication over time.  Like most skills, great science communication comes with practice...process of writing papers and proposals.  To get the most out of these interactions, ask yourself how every...you intended. 3. Join the scicomm community Like most aspects of science, communication isn't easy.  But...

...here are the basic requirements of use. First, and most obviously, you need a non-canonical amino acid, ...codon used is often one of the three stop codons ­– most commonly the UAG codon – in some systems, rare codons... with systems and ncAAs that were, in practice, mostly accessible to experts. That many GCE technologies...accessible to your lab. The solution GCE provides almost endless possibilities for researchers, from selective...own labs. They provide resources, knowledge, and most importantly, validated protocols (using readily ...

...Curious which viruses researchers have found the most useful so far? We crunched the numbers on our Viral...Service (and then we crunched them again) to find the most requested lentivirus and AAV of 2016. The top viruses...drumroll please)... Top Lentivirus: lentiCas9-Blast Most of Addgene’s lentiviral inventory is based on CRISPR...non-CRISPR related viral preps. Cas9 is the headliner of most CRISPR applications and can be expressed from your...Gi)-mCherry Our current AAV inventory consists almost entirely of viruses expressing DREADDs from Bryan...

...Congratulations Mark Mellett! Siddharth Prakash: X chromosome genes involved in Turner syndrome Turner syndrome... a developmental disorder where one of the X chromosomes is partially or completely missing in women. ... cells from women who are missing an entire X chromosome (TS iPSCs), the lab observed abnormal cell migration...developmental defects. Eight genes from the X chromosome are expressed at significantly reduced levels...reagents will facilitate our aims to correct X chromosome gene dosage in TS cells as a first step to reverse...

...As a scientist, perhaps the most important part of your research is communicating the results of the ...different amounts of access or feedback. One of the most significant shifts in scientific communication is...part of their scientific communication needs. The most often-cited reasons researchers might not post a...biases or errors, with a lack of transparency in most review processes. Preprints and their feedback, ...discussed and addressed by the scientific community. Most preprints are eventually published in journals after...

...that can replicate independently from its host’s chromosome. Although we usually think of bacteria when we...Eukaryota can also harbor these circular DNA passengers. Most natural plasmids encode beneficial traits to entice... cellular reproduction, similar to the host’s chromosome, or they can spread between hosts through horizontal...factors can also be integrated into the bacterial chromosome, but when encoded on plasmids, they can promote...Eren, A. M. (2024). A cryptic plasmid is among the most numerous genetic elements in the human gut. Cell...

...Submit your grant early! First off, and perhaps the most important bit of advice I can suggest, is to give... your application and give you feedback. They’ve most likely been through the process themselves many ...Originality is essential for grant success The two most important things reviewers will be looking for in... worth knowing? This can sometimes be tricky, as most of our research builds on ideas and findings from...explanation of your research for non-scientists. Most fellowships are funded by the general public (either...

...service interaction - one that gives our customers the most utility in the least amount of time. Though I now...accumulated wisdom so that you, the customer, can get the most out of your email or phone call. Though my experience...times, I’m pleasantly surprised to discover that the most crucial information about a product is printed right...asked by customers (and I expect this is the case at most companies). Always go back to the source. Although...Following the tips above should help you get the most out of your interaction.   Resources on the Addgene...

...fragments together in a one-tube reaction. Even though most MoClo reactions use Golden Gate cloning, not all...are defined DNA sequences with specific functions. Most individual parts have characterized activities with... with predictable behaviors. While a gene is the most obvious example of an individual part, this category...review a list of MoClo applications. Genetic circuits Most things in biology are not so simple, and engineering...delivered) in certain model organisms. In mammals, most CRISPR libraries are assembled and delivered virally...

...antagonistic and impolite, Complainers, who spend most of their time moaning about their troubles, Silent... are a few principles that will help you resolve most conflicts, even with difficult people. If I had ... Put important agreements in writing One of the most frequent sources of conflict is miscommunication...or just an inexperienced manager, you can resolve most conflicts if you communicate your ideas assertively...www.FinishYourThesis.com.   Additional Resources Getting the Most Out of Supervisor Meetings by Joanne Kamens on  ...

...interesting. If people could be against GMOs as food but support GMOs that result in a new utility, was there...presence. They are genetically modified organisms, and GMOs have a bad reputation. The public is can be fearful...comments were more often along the lines of “I hate GMOs, but what you are doing makes sense. I can support...will need, and the business training to make the most of it. It has also allowed us to really analyze ...

...hood is in use. Wipe the hood with a disinfectant (most people use 70% ethanol, but you can also use Incidin...contamination. This sounds rather trivial, but often solves most problems before they occur. With proper planning...contact with the cells should already be at 37 °C. Most labs have a water bath constantly at 37°C for this... to form a routine. Twice a week often works for most fast-growing cell lines (such as HEK293, which multiplies... for experiments requiring reproducible results. Most cell culture media contain the pH indicator phenol...

...bacterial host cell is almost always the answer. E. coli is definitely one of the most popular hosts for protein... that make up the primary structure of proteins. Most proteins are then modified via an array of post-...post-translation modifications for proper protein function. Most DNA constructs designed for mammalian expression...used for protein expression, S. cerevisiae, is the most reliable and frequently used species due to its ...

...including interference, activation, and knockout. Most CRISPR screens rely on lentiviral systems, a fact...immunogenicity, AAV still beats lentivirus. In mice, AAV is almost entirely non-immunogenic — meaning when conducting.... Lentivirus has limited packaging options, with most lentiviral vectors being packaged using VSV-G. In...which means that when using Cas9, you’re already almost out of space! Don’t let this get you down for too...have been found to get around this restriction. The most obvious workaround is to use a multi-plasmid system...