We narrowed to 610 results for: gan
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TypeBlog Post...your organization Public Speaking Time management Managing a team Conflict resolution Organization Leadership...than you thought! When change happens at your organization, when your boss needs someone to step up, when...likely collected some great time management and organizational skills. If you are responsible for working ...students, managing inventories in the lab, or organizing a research project, one can assume that you’ve...
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Plasmids 101: Gateway Cloning
TypeBlog Post...variety of model organisms. Use the links below to find Gateway plasmids for your organisms of interest: ... will depend on a number of factors, like your organism, desired expression level, and experimental purpose... Genomic DNA and is available for all kind of organisms from mammals to E. coli. Glossary of Gateway cloning.... A Genetic Switch: Phage (Lambda) and Higher Organisms (Cambridge, MA: Cell Press). Additional Resources... -
Fluorescent Protein Guide: FRET
TypeCollection... not required to be fluorescent. A variety of organic dyes and fluorescent proteins have been developed... -
Viral Vectors
TypeCollection... this genetic material to specific cells in an organism. Figure 1. Using viral plasmids to generate virus... -
Thoughts on the Future of Research 2015 Boston Symposium
TypeBlog Post...the more detailed questions the organizers wanted to touch on in organizer David Riglar’s blog post. Essentially...Symposium On the FOR website, the FOR Boston 2015 organizers indicate that the main question they wanted to...panel was that there are many companies and organizations (including Addgene), that need people who are...questions, panelists and participants of the symposium began providing means to answer some of these questions... -
CRISPR Plasmids - Mammalian Expression
TypeCollection...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR... -
CRISPR Plasmids - Plants
TypeCollection...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR... -
Transferable Skills Guide: Teamwork
TypeBlog Post...senior year isolating pheromones from various C. elegans mutants by myself, I would have slowly gone crazy...contribute to the Management Team that steers the organization. As an Addgene vet familiar with the expertise...like-minded group of co-workers who want to make our organization as environmentally friendly as possible. We ...make Addgene the most environmentally friendly organization on the planet, in which case whoa! Mission accomplished...Management Skills Volunteering at a Professional Organization Resources on Addgene.org Check out Career'... -
Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors
TypeBlog Post... on the advisory board for Art in Giving, an organization that raises money for childhood cancer through...art. About two years ago, I had a big role in organizing an Art in Giving think tank event where we brought...proposal, which Art in Giving was able to fund. Organizing that event and seeing the outcome was wonderful...wonderful! I also volunteer for a dog rescue organization where I foster dogs and help screen dog adoption... -
Why Do I Need an MTA Anyway?
TypeBlog Post... for more details. Benefits to the provider (organization / lab that owns the material) Affirmation ...materials will only be shared with non-profit organizations Assurance that recipient may not use the ...Limited liability Benefits to the Recipient (organization / lab receiving the material) Permission ...directly to your institution’s MTA coordinator. Organizations that have opted into our eMTA system can even... -
Let There Be LITE Plasmids
TypeBlog Post...tissue. “The brain is such an amazing and dynamic organ,” Konermann explained. “It helps us adapt to influences...use is by no means limited to particular model organisms or cell types. “This tool is really flexible and... making use of the new optogenetic tools in C. elegans and in Plasmodium. For those who do want to apply... Kendall Morgan... -
With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog Post...research is vital in combating misinformation and propaganda that limits access to full reproductive health...-based policy and legislation hit home hard in Michigan, where programs like the Great Lakes Restoration...planet. And as we rallied on the University of Michigan campus speakers thoughtfully reminded us to use...a postdoctoral researcher in the University of Michigan Department of Astronomy. She studies how planets...I was also incredibly impressed with how well organized the march was, as well as the friendly atmosphere... -
Science as Art
TypeBlog Post... is essential to stay in touch with the living organism I study, to be able to see it all scales. Most... a way to better understand and appreciate the organism, for both scientist and non-scientist alike. “...inner structures of delicate plants and other organisms without destroying the original, which could provide...Do you look for the beauty in the microscopic organisms on your lab bench? Let us know in the comments... -
Mapping the 4D nucleome with CRISPR/Cas9
TypeBlog Post... the organization of the nucleus in space and time, and to understanding how nuclear organization varies...varies across the life of a cell, or how organization may be altered in disease states. dCas9 gets a colorful...work to increase our understanding of chromatin organization. This fluorescent CRISPR/Cas9-based method is... -
Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes
TypeBlog Post...Fluorescent reporters are not as well suited for imaging organs located deep in the body, like the gastrointestinal...the reporter and fail to pinpoint what tissue or organ this signal is coming from. Acoustic reporter...where the bacteria localize in relation to internal organs. The limit of detection for arg1 expressing ECN...Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian hosts. Nature, 553, 86-90. PubMed... -
Safe Port in a Storm...How Addgene is Weathering the Pandemic
TypeBlog Post...succeed at the same time. I began approaching science funding organizations to request support for our ..., cures, and diagnostics. Almost immediately we began to receive urgent requests for access to coronavirus-related...were facing a financial crisis. As a nonprofit organization, Addgene charges enough for our services to ... -
Meet the New Addgene Board Members!
TypeBlog Post...,” Baradaran says. Baradaran’s path to science began in high school. “I had a great chemistry teacher...this is what I wanted to do,” Stegmeier says He began this journey in biochemistry made the foray into... at Addgene. While deposits from for profit organizations are less common, they can be very impactful.... diseases and over the past few months, the lab began a project to develop an AAV-based COVID-19 vaccine... -
Multifunctional Neural Fibers: Modulation from Gut to Brain
TypeBlog Post...tool to advance our knowledge of brain-visceral organ signaling. The problem Previously, there were... for monitoring how both the brain and visceral organs interact during various behaviors. Either tissue...common neuroscience tools into the brains of model organisms, including the Anikeeva lab’s work on a similar...applications are to expand the tool to different brain-organ axes. Alternatively, the system could be used to... -
Viral Vectors 101: Transductions
TypeBlog Post...depends on two things. First, what type of cell or organism context you are targeting? Second, what is the...moderate levels. With regard to target cells or organism, some primary cells and differentiated cell types...shine is when a specific cell type within a live organism is the target. Although rarely done, viral pseudotype...for a type or subtype of cells. This allows an organism to be injected with a virus, but enables tissue-specific... -
Virtual Science Conference Coming Up? Three Addgenies Share Their Experience
TypeBlog Post...Everyone workshop at TAGC with the three other organizers. We asked them about their experiences as they...Reproducibility for Everyone Workshop, Abitua and the other organizers found several avenues to keep the audience engaged...conference was over. Depending on the conference, organizers may make the poster available after the conference...actively reach out. It’s more proactive versus organic networking,” she says.Depending on the meeting,...