Better Dyeing Through Chemistry & Small Molecule Fluorophores
Type
Blog Post
...protein and (2) a synthetic fluorophore-contianing “ligand” that binds to the tag. Like most good ideas in...selective interaction between a bisarsenical dye ligand (e.g., FlAsH, ReAsH) and a short genetically encoded...specifically and irreversibly with a small substrate ligand motif attached to a fluorophore (Figure 1b).
...catalyze the covalent attachment of a fluorophore ligand to a peptide tag (Figure 1c).
Click chemistry...labeling strategies is fluorogenicity, meaning the ligand exhibits low fluorescence when free in solution...the availability of fluorescent and fluorogenic ligands.
New fluorophores
As a corollary to these innovative...