...been an invaluable resource for studying the organization and function of cellular systems. FPs have been...engineering FP fusions associated with cellular organelles, scientists have been able to study many cellular... by evaluating visual phenotypes. Improving C. elegans fluorescent protein tagging with SapTrap The Jorgensen...SapTrap to improve CRISPR genomic tagging in C. elegans. SapTrap offers scientists a convenient one tube...tagging systems For those who do not work with C. elegans, several groups have designed modular toolkits ...CRISPR/Cas9 Gene Modification in Caenorhabditis elegans.” Genetics. 202(4) (2016):1277-88. PubMed PMID:...

...protein and (2) a synthetic fluorophore-contianing “ligand” that binds to the tag. Like most good ideas in...selective interaction between a bisarsenical dye ligand (e.g., FlAsH, ReAsH) and a short genetically encoded...specifically and irreversibly with a small substrate ligand motif attached to a fluorophore (Figure 1b). ...catalyze the covalent attachment of a fluorophore ligand to a peptide tag (Figure 1c). Click chemistry...labeling strategies is fluorogenicity, meaning the ligand exhibits low fluorescence when free in solution...the availability of fluorescent and fluorogenic ligands. New fluorophores As a corollary to these innovative...

...annotating, and storing life science protocols. Since we began sharing this protocol on the Addgene website in ...About Developing Lab Management Software    Organizing and sharing your updated protocols So that’s ... mobile) tools are available for scientists to organize and share their scientific protocols. Here are...OpenWetWare (see below), and now Addgene. I just began uploading our cloning protocols to the site and ...streamline your lab management and you want the works: organizing reagent storage and inventory, managing orders... can offer a one-stop solution to your lab's organization and management needs. With Quartzy you can share...

...but, ummmm, what is it? Reddit is a volunteer-organized platform where users can participate in forum ...trolls. 3. The AMA process was simple and well organized. You can check out the r/Science AMA Submission...only small downside to the AMA from my end as an organizer/promoter was that I didn’t have the direct link... science talk here). Finally, my main content organizer is the amazing app Evernote. I have a paid account...which gives me all the features I need to save, organize and easily find the resources that I come across...do without it. So get yourself a good content organizer and build your own professional development etc...

...Using a technique called systematic evolution of ligands by exponential enrichment (SELEX), some 1010 oligonucleotides...of the next two decades, these nucleotide-based ligand binders would prove to be highly adaptable tools...structure (see Fig. 1a). When the aptamer binds its ligand, the stem loop is disrupted, removing the quencher... dimerization for activation, only in this case ligand binding would bring the relevant ends together ...aptamers fluoresce until they bind to the appropriate ligand, but relying on a lack of fluorescence for readout...response. Aptamers can be selected for these small ligands. References 1. Ma, Hanhui, et al. “Multicolor...

...Collection are sorted by organism (see screenshot above). Don’t see your favorite organism listed? Help us serve...serve you! Let us know about other organisms we should include by emailing us at [email protected]. If...to narrow your keyword search to one of these organisms. Help us continue to develop resources for your...like to get the word out about a particular microorganism, microbial technique, or microbial technology...

... ease of use and utility in a wide variety of organisms.  While much of current CRISPR research focuses... plant species, including commonly used model organisms like Arabidopsis and Medicago truncatula and several...universal functionality of the CRISPR system in most organisms, some plant-specific changes to CRISPR components...experimental design principles developed in other model organisms (see our CRISPR guide for common CRISPR principles...sequence) is consistent between plants and other organisms, but the promoter used to drive gRNA expression...your gene of interest into a target cell line or organism (Presented in figure 1). More information on Agrobacterium-mediated...

...critical in the business world. Companies and organizations that avoid all conflict can fall into “groupthink...individuals involved, and also for the labs, organizations, or businesses as a whole. When I’m attempting... sharing gets more challenging the bigger an organization gets, and creating new avenues of communication...should be communicated is necessary in a large organization, because confirming that every person has read...attempting to resolve conflicts within a given organization, it can help tremendously to have as much context... solutions that benefit themselves and their organization. Resolving conflicts is great, but avoiding ...

...Optogenetics gives you the power to control cells or organisms with the flip of a switch. You may be familiar...chromosomes during mitosis, support transport of organelles and vesicles, and more. Although several strategies...that means this light-powered sword truly is an elegant tool. The authors demonstrated its use to probe...family proteins support vesicle trafficking and organelle dynamics, but with dozens of family members and...forming the cilia that help establish left-right organization of the embryo (Aljiboury et al., 2023). It’s...and cilia formation during zebrafish left-right organizer development. PLoS Genetics, 19(5), e1010765. https...

...conference here. The Future of Research conference is organized by postdocs for postdocs. I am delighted to see...importance of the topics to be discussed, the organizers are building valuable skills and meeting new ...Students also partner with independent career organizations to get the grants and programs they need such...offices in the last few years. However, some organizations are woefully behind and some just don't have...Symposium on the Future of Science: A postdoc-organized symposium on the future of the scientific endeavor...

...through your organization in this field. Please also enter the billing address for your organization. We will...By Natalie Mortenson and Laura O'Regan Ordering jeans online from your favorite store is easy. Ordering...you time. Anyone affiliated with the recipient organization can create an Addgene account and place an order... entered during checkout. At this time, your organization will have net 30 days to complete payment for...

...as a free event! Organizing a science conference for the public How does one organize a public scientific...association with the Harvard graduate student organization Science in the News (SITN), we recently sponsored...communication skills and engage with the public. The organization runs a wide variety of programs, including fall...opportunities to improve our communication and organizational skills provide great training for a number ...

...Popular Model Organisms by Alyssa Cecchetelli and Lukas Morgan There are many model organisms people are...interview including defining your research vision, organizing your vision into a chalk talk, and tips for practicing.... In this blog post, we’ve covered five model organisms: mouse, fruit fly, yeast, zebrafish, and worm....

...the bench, I have worked for seven different organizations in a variety of science policy roles including...Big P policy from a post in non-governmental organizations, universities, professional societies, and industry...is non-governmental work happening at large organizations who have to develop policies for their communities...on a neglected disease area. Depending on the organization, these changes to policy could shift how society...your time in graduate school through outreach organizations and science blogs. Other avenues for skill development...development include leadership of extracurricular organizations, working in student government, and teaching...

...Cambridge and within the general public. The society organises talks by prominent scientists, including Nobel...topics in their area of research. The society also organises PhD information events and summer internship information...support each other and provide advice. The society organizes numerous recreational and educational events throughout...for their students. Another large goal of the organisation is to encourage iGEM alumni to mentor younger...Bloggers Learn How to Get Involved in a Profession Organization ...

...mouse, Drosophila, C. elegans, and zebrafish – essentially all of the standard organisms for which extensive... as T lymphocytes. Griesbeck and his colleagues began by isolating a calcium-binding protein, troponin...whose association is disrupted in the presence of a ligand (they stick together in one state, but not the ... Kendall Morgan...

...is a big job, but under the leadership of Nevan Krogan at UCSF we’ve brought together a network of scientists... figure out what is going on there!).  Find the Krogan Lab SARS-CoV-2 plasmids (Note: The Nsp6 construct...available shortly. Sign up for email alerts on the Krogan lab's Addgene page to get notified!) Attacking ...FedEx envelopes waiting to be shipped out of the Krogan lab at UCSF. Our team members (shout out to ...be sure to follow us on Twitter (QBI_UCSF and KroganLab), Instagram, and Facebook. Find more COVID-19 ...

... bench scientist who began working remotely recently? Most of us at Addgene began working from home last...for all ages. Organize your data and prepare publications or your dissertation Organize data that you...begin working on these drafts and for a few model organisms, Micropublication will accept negative data or...

...modular cloning? Modular cloning, or MoClo, is an organization scheme for multi-part DNA assembly. Simply put...’s because MoClo specifically refers to the organizational schema described above, and not any particular...other non-Golden Gate cloning methods. MoClo’s organization schema MoClo can be broken down into three hierarchical...many guides/parts delivered) in certain model organisms. In mammals, most CRISPR libraries are assembled...targeting constructs and delivering them to these organisms via another optimized platform. Gene Stacking ...

...your organization Public Speaking Time management Managing a team Conflict resolution Organization Leadership...than you thought! When change happens at your organization, when your boss needs someone to step up, when...likely collected some great time management and organizational skills. If you are responsible for working ...students, managing inventories in the lab, or organizing a research project, one can assume that you’ve...