We narrowed to 323 results for: cloning

The origin of replication is the DNA sequence which allows initiation of replication within a plasmid by recruiting transcriptional machinery proteins, enabling a plasmid to reproduce itself.

The COVID-19 pandemic makes open science more important than ever. Read about how Addgene is weathering COVID-19 with support from the scientific community.

Learn about the basic elements that make up the AAV transfer plasmid, including the ITRs, transgene, promoter, and other cis-regulatory elements.

Find details on five methods to quantify DNA: UV absorbance, fluorescence dyes, agarose gel electrophoresis, capillary electrophoresis, and diphenylamine.

Learn how you can use the Build Your Plasmid card game to teach undergraduate students about genetic engineering.

Learn about SnapGene's feature database and how it can be used to identify sequence elements in your plasmids.

Read advice for grad students and postdocs applying for science careers in sales and marketing, including tailoring your CV.

Browse Addgene’s curated list of CRISPR resources and references, including gRNA design tools, CRISPR FAQs, and community forums.

A review of K. phaffii as a model organism and its relevant applications for biotech to basic research.

Find out what CRISPR tools are distributed as a part of Addgene's new viral service.

Take a look at these 5 great apps to make your lab life easier including tools from Gibco, LabArchives, NEB, ThatsMyStapler, and iGreenEarth.

A look at the challenges facing the field of plant synthetic biology, including a discussion of changing public perception, IP concerns, and more.

Find CRISPR plasmids to study the regulatory networks involved in retinal development.

CRISPR Cas9 gRNA plasmids from the Zhang lab

Addgene's microbiology resources: Find plasmids for your microbe of interest, including bacteria, viruses, protozoa, fungi, and more.

A selection of Addgene's empty vector backbones curated by species, epitope tags/fusion proteins, function, selectable markers, and more.

Learn more about synthetic biology resources available at Addgene. Browse featured collections or depositing labs.

A review of protein expression mechanisms for on, off, and tunable, control of protein expression.

Get the basics on monoclonal antibodies including how monoclonal antibodies are made and why use monoclonal antibodies for your experiment.

If you’re using CRISPR to make a genome edit, how do you know if your experiment was successful? Try using fluorescent CRISPR reporters like SRIRACCHA and GEmCherry2.