Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 326 results for: cloning

Showing: 161 - 180 of 326 results

  1. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Independent Cloning (LIC) pAG CMV Empty Puro - Empty mammalian expression plasmid for cloning untagged proteins...Lentiviral sgRNA cloning backbone pLKO.1-blast - 3rd gen lentiviral backbone for cloning and expression ...may contain tags or fusion proteins, a multiple cloning site (MCS), an inducible promoter, and/or a selectable...with C-terminal mTagBFP2 tag pNIC-GST-TEV-GG - Cloning of target gene with N-terminal GST tag and TEV ...for bacterial expression, Ligation Independent Cloning (LIC) Also see our Bacterial Expression collection...zebrafish insulin promoter vector with multiple cloning sites Browse our full Fluorescent ...thioredoxin) Protein purification pNH-TrxT - LIC cloning in bacteria with N-terminal His-TrxA-TEV tag NusA...

  2. Microbiology Resources

    Type
    Collection
    ...- Barbier Lab Cloning and Expression Bacteria and yeast expression vectors : FX cloning System - Geertsma...Parts Kit : Molecular cloning for E. coli - Densmore Lab EcoFlex MoClo : Modular cloning for applications ... and Dutzler Labs EasyClone 2.0 Yeast Toolkit - Borodina Lab Golden GATEway cloning system - Wittbrodt...Microbiology : Sets of plasmids for fluorescence, cloning and expression, and synthetic biology. CRISPR Resources...sets of plasmids intended for use together (e.g. cloning and expression systems) and therefore supplied ...

  4. Antibody Plasmid Collection

    Type
    Collection
    ...well as by our trusted partner labs. Learn More Cloning and Expressing Antibodies Antibody Plasmids Antibodies...synthetically produced recombinant antibodies, created by cloning antibody components into plasmids and expressing...Monoclonal antibodies are derived from an individual clone targeting a single antigen. An example of R-mAbs...and their applications in our Antibody Guide ! Cloning and Expressing Antibodies The following table highlights...mammalian expression vector A tool kit for rapid cloning and expression of recombinant antibodies. Sci Rep... Rep. 2014 Jul 30;4:5885. Andrew Beavil Cloning and mammalian expression of mouse and human IgG monoclonals...

  5. TALEN Plasmids and Kits

    Type
    Collection
    ...336 pFusX clones allowing for Golden Gate assembly of 15-16 RVD TALEN inserts in one cloning step rather...Stephen Ekker Golden Gate TALEN 2.0 Golden Gate cloning method. Validated in multiple organisms. Dan Voytas...Keith Joung Zhang Lab TALE Toolbox PCR/Golden Gate cloning method. Optimized for human expression. Feng Zhang... Assembly Kit Assembly by ligation-independent cloning (LIC). Validated in human cells. Veit Hornung Musunuru...plasmids sequence positions 1214–2210 of pTAL3 were cloned into a pCS2 expression vector resulting in shorter...RR) used are obligate heterodimers that require cloning of left and right TALEN monomer proteins into opposite...enhancers in mammalian cells. These plasmids allow for cloning DNA fragments encoding TAL effector repeat arrays...

  6. Synthetic Biology - Overview

    Type
    Collection
    ...Proteins FREQ-Seq Kit FX Cloning Kit GreenGate Cloning System (Plant) MoClo Modular Cloning Modular PUF Assembly...Plasmids by Category Cloning and Genomic Tools Browse plasmids related to cloning and genomic modification...

  8. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...two vectors are part of the larger pMVP/pMAGIC cloning system, which contains Cas9 fusions for several...Arlotto ME, et al (2018) Creation of versatile cloning platforms for transgene expression and dCas9-based...

  9. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...researchers to insert targeting oligos with minimal cloning required. As with other model systems, multiple...containing vectors ready for Golden Gate mediated cloning of your gRNA of interest. Figure 1: Simplified...

  10. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...cell lines is to transfect, select, and isolate clones. This procedure is cumbersome and inefficient, ... somewhere between one hundred and one thousand clones to find a cell line with the edit of interest. ...

  11. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ... Protocols Plasmid Cloning by PCR Plasmid Cloning by PCR You may also...Designing Primers for PCR Based Cloning The basic PCR primers for molecular cloning consist of: Leader Sequence... for primer design: Because we are cloning an ORF, we want to clone from the start codon (ATG) to the ...PCR based cloning carries a much higher risk for mutation than restriction enzyme based cloning. DNA replication...Protocols for plasmid cloning by PCR. Protocols...Ligation Bacterial Transformation Summary PCR based cloning is incredibly versatile and allows for nearly any...limitations. Background In its simplest form, PCR based cloning is about making a copy of a piece of DNA and at...

  12. Bacterial Expression Systems

    Type
    Collection
    ...resistances. Comprehensive multi-cloning site compatible with standard cloning using many different restriction...Tn7 Transposition Nancy Craig A gene of interest cloned into one of these plasmids can be transposed into.... pET-HIS-Sangamo 40786 TALEN Gang Bao Gateway cloning and expression of an N-terminally His-tagged TALEN.... pET-Sangamo-His 40787 TALEN Gang Bao Gateway cloning and expression of a C-terminally His-tagged TALEN...His tag and an MBP tag. Compatible with Gateway cloning. pDEST-periHisMBP 11086 Tac (lactose/IPTG inducible...formation or prevent toxicity. Compatible with Gateway cloning. pGTvL1-SGC 39188 pTac (lactose/IPTG inducible)... and TEV cleavage site. Includes sites for LIC cloning, and a "stuffer" fragment that includes the SacB...

  13. Ligation Independent Cloning

    Type
    Protocol
    ... Protocols Ligation Independent Cloning Ligation Independent Cloning You may also ...Independent Cloning (LIC) obviates the need for the time-consuming ligation step of traditional cloning methods... Independent Cloning Protocols...also like... Cloning Methods Empty Backbone Guide Molecular Biology Protocols Overview Ligation Independent...methods. In traditional cloning, base-pairing in the short overlapping regions (usually 4 bp) does not provide... restriction sites from the final product (no "cloning scars"). A “stuffer” sequence allows for electrophoretic... and may provide counter-selection for negative clones as in the example shown here. The manufacturer ...

  16. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...Plasmid Cloning Name Description (Link opens in a new window) Link to Video Restriction Cloning Assemble...ligation Cloning by PCR Generate restriction sites by PCR Modification by Annealed Oligo Cloning Add a short...single reaction Ligation Independent Cloning (LIC) Scarless cloning with Type II restriction enzymes and...and T4 polymerase pLKO.1 - TRC Cloning Vector Cloning protocols for using the pLKO.1 vector, a backbone...can be used for basic molecular biology, plasmid cloning, and titering and testing your viral preparations... for many more complicated procedures. Plasmid Cloning Protocols for constructing and analyzing your plasmids...

  17. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ...PCR - or restriction enzyme -based cloning to test individual clones before use of more expensive forms...digested with restriction enzymes common to multiple cloning sites, will result in similar sized bands, thus...Insert Orientation by Restriction Digest If you clone an insert into a vector by a single restriction ... enzyme, you will need to verify that it has be cloned in the correct orientation - this can be done by...restriction digest. Although this is never an ideal cloning strategy, sometimes it cannot be avoided. If you...therefore the only thing left to do is identify the clone(s) in which the insert is in the correct orientation... want to know how to differentiate between two clones that differ only in the orientation of the insert...

  18. Plasmid Modification by Annealed Oligo Cloning (with Protocols)

    Type
    Protocol
    ...Changing a Multiple Cloning Site (MCS) Adding short tags - ex: HA or Flag Cloning shRNAs Background For...using oligo overlap cloning. Protocols... Protocols Plasmid Modification by Annealed Oligo Cloning Plasmid Modification...Modification by Annealed Oligo Cloning You may also like... Restriction Digest of Plasmid DNA DNA Ligation Bacterial...Bacterial Transformation Summary Oligo overlap cloning can be used anytime you need to add a short stretch...NdeI, PacI, AscI, and MfeI. With oligo overlap cloning, you can design a set of oligos containing your...design overlapping oligos that once annealed can be cloned directly into the overhangs generated by restriction...

  20. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...Options”, including adding adapter sequences for cloning purposes.  Press run and wait for the generation...this purpose.  The gRNA oligos are subsequently cloned into your vector of choice using the adapter sequences...
Showing: 161 - 180 of 326 results