...Proteins FREQ-Seq Kit FX Cloning Kit GreenGate Cloning System (Plant) MoClo Modular Cloning Modular PUF Assembly...Plasmids by Category Cloning and Genomic Tools Browse plasmids related to cloning and genomic modification...

...336 pFusX clones allowing for Golden Gate assembly of 15-16 RVD TALEN inserts in one cloning step rather...Stephen Ekker Golden Gate TALEN 2.0 Golden Gate cloning method. Validated in multiple organisms. Dan Voytas...Keith Joung Zhang Lab TALE Toolbox PCR/Golden Gate cloning method. Optimized for human expression. Feng Zhang... Assembly Kit Assembly by ligation-independent cloning (LIC). Validated in human cells. Veit Hornung Musunuru...plasmids sequence positions 1214–2210 of pTAL3 were cloned into a pCS2 expression vector resulting in shorter...RR) used are obligate heterodimers that require cloning of left and right TALEN monomer proteins into opposite...enhancers in mammalian cells. These plasmids allow for cloning DNA fragments encoding TAL effector repeat arrays...

...to add the CACC and AAAC cloning overhang. When designing oligos for cloning my target sequence into a... discriminate between positive and negative clones. Clones with insertion will show only linearized plasmid...Should I add the PAM sequence to the oligo when cloning my target spacers into the PX330 vector backbone...together, with each plasmid having a targeting spacer cloned into the backbone ( pX330 or PX335 , depending ... to target two genomic loci with wildtype Cas9, clone two spacers for each locus into PX330, then co-transfect...concern, you could go ahead with PX330 plasmid, clone in your target guides, test run them and then select... the Bench FAQs How can I quickly check if the cloning of my oligo into a backbone like PX330 worked? ...

...protocol. Or maybe you can’t find the molecular cloning guide you were sure was in one of those four white...

...resistances. Comprehensive multi-cloning site compatible with standard cloning using many different restriction...Tn7 Transposition Nancy Craig A gene of interest cloned into one of these plasmids can be transposed into.... pET-HIS-Sangamo 40786 TALEN Gang Bao Gateway cloning and expression of an N-terminally His-tagged TALEN.... pET-Sangamo-His 40787 TALEN Gang Bao Gateway cloning and expression of a C-terminally His-tagged TALEN...His tag and an MBP tag. Compatible with Gateway cloning. pDEST-periHisMBP 11086 Tac (lactose/IPTG inducible...formation or prevent toxicity. Compatible with Gateway cloning. pGTvL1-SGC 39188 pTac (lactose/IPTG inducible)... and TEV cleavage site. Includes sites for LIC cloning, and a "stuffer" fragment that includes the SacB...

...yourself or you could “clone by phone”. One of my professors was excellent at phone cloning with labs around...

...cell lines is to transfect, select, and isolate clones. This procedure is cumbersome and inefficient, ... somewhere between one hundred and one thousand clones to find a cell line with the edit of interest. ...

... expression, Ligation Independent Cloning (LIC) Bacteria Lac, T7, araBAD p15TV-L - His-tagged... Plasmids - More LIC bacterial cloning vectors pTD plasmid ...GFP-tag fusions for transcription pCSDest - Easy cloning of your gene using Gateway recombination... GFP for mammalian expression pcDNA3 GFP LIC cloning vector - C-terminal GFP for mammalian...Retroviral gene expression pLKO.1 - TRC cloning vector - Lentiviral shRNA expression... - Mammalian retroviral Gateway cloning vectors Adenoviral High transduction efficiency...RNAi For gene silencing experiments pLKO.1 - TRC cloning vector - Lentiviral plasmid for...

...target species, target name, host organism, isotype, clone details, vendor, method, tissue preservation, target...

...Formosa has deposited six plasmids with a multiple cloning site in place of the epitope tag for creation of...

... PI 10878 pLKO.1 ‐ TRC cloning vector shRNA expression; Recommended for cloning new shRNAs (contains a...Below, browse our selection of empty vectors for cloning in shRNAs. Plasmids for constitutive expression...cassette from another vector (e.g. pSUPER) can be cloned into this vector; Contains a 5'LTR and should be...Read our detailed protocol for using the popular cloning vector pLKO.1. Trono Lab Lentivectors Toolbox Tyler...(CAG promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned from pLVTHM) On Patrick Aebischer and Didier Trono...(CAG promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned from pLVTHM) Off Patrick Aebischer and Didier ...1alpha promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned from pLVTHM) On Patrick Aebischer and Didier Trono...

... Protocols Plasmid Cloning by PCR Plasmid Cloning by PCR You may also...Designing Primers for PCR Based Cloning The basic PCR primers for molecular cloning consist of: Leader Sequence... for primer design: Because we are cloning an ORF, we want to clone from the start codon (ATG) to the ...PCR based cloning carries a much higher risk for mutation than restriction enzyme based cloning. DNA replication...Protocols for plasmid cloning by PCR. Protocols...Ligation Bacterial Transformation Summary PCR based cloning is incredibly versatile and allows for nearly any...limitations. Background In its simplest form, PCR based cloning is about making a copy of a piece of DNA and at...

...currently unavailable, you will need to design and clone in a gRNA(s) to guide the Cas9 protein to your target... target sequence, as well as design and clone in the homology arms for the donor plasmid. The Mendenhall... also provided their protocol for homology arm cloning: Mendenhall & Myers FETCh-seq Protocol 134.7 KB... complexes. This vector serves as a backbone to clone the left and right homology arms. More details on...al. Cell Rep. 2015 . Alternatively, cDNAs can be cloned directly into this vector and targeted to the AAVS1...include Cre and FLP expression vectors, a general cloning plasmid, and a prebuilt Unc-32::GFP targeting vector...

...plasmids The transfer plasmid is the vector you clone your gene of interest into and is an important feature...Lentiviral production and transduction Once you have cloned your gene of interest into lentiviral transfer ...

...easy creation of long hpRNA with Gateway-based cloning and straightforward positive selection of plants...assembly of intron-spliced hpRNAs through Golden Gate cloning. Artificial micro RNA and short hairpin RNA The...

...Options”, including adding adapter sequences for cloning purposes.  Press run and wait for the generation...this purpose.  The gRNA oligos are subsequently cloned into your vector of choice using the adapter sequences...

... Protocols Ligation Independent Cloning Ligation Independent Cloning You may also ...Independent Cloning (LIC) obviates the need for the time-consuming ligation step of traditional cloning methods... Independent Cloning Protocols...also like... Cloning Methods Empty Backbone Guide Molecular Biology Protocols Overview Ligation Independent...methods. In traditional cloning, base-pairing in the short overlapping regions (usually 4 bp) does not provide... restriction sites from the final product (no "cloning scars"). A “stuffer” sequence allows for electrophoretic... and may provide counter-selection for negative clones as in the example shown here. The manufacturer ...

...Plasmid Cloning Name Description (Link opens in a new window) Link to Video Restriction Cloning Assemble...ligation Cloning by PCR Generate restriction sites by PCR Modification by Annealed Oligo Cloning Add a short...single reaction Ligation Independent Cloning (LIC) Scarless cloning with Type II restriction enzymes and...and T4 polymerase pLKO.1 - TRC Cloning Vector Cloning protocols for using the pLKO.1 vector, a backbone...can be used for basic molecular biology, plasmid cloning, and titering and testing your viral preparations... for many more complicated procedures. Plasmid Cloning Protocols for constructing and analyzing your plasmids...

...promoter. WPRE has been removed to increase the cloning capacity. pCDH-CB-IRES-copGFP-T2A-Puro 72299 Express...well as the Cre and Flp recombinases. Genes can be cloned into these transfer vectors using shuttle vectors...pAdx-CMV-LacZ is uniquely suited to simplify the cloning process as it can be used in blue-white screening...Xgal/IPTG plates – if your insert is successfully cloned into this vector, transformants will instead form...

....1 – TRC cloning plasmid 3rd U6-driven shRNA empty plasmid; includes a stuffer for easy cloning Root 14748...shRNA. See article for detailed information about cloning & additional plasmids. Aebischer and Trono 11795...cassette (promoter and gene of interest) can be cloned into the plasmid. Nolan 12257 pWPXL 2nd EF-1alpha... the gene of interest, also can express shRNA (cloning H1-shRNA cassettes into the unique SnaBI site in...