...Plasmids Find Fluorescent Protein Kits Find Empty Backbones with Fluorescent Protein Tags ...

...disease. “My mentors Dr. Dianna Milewicz, Dr. Carolyn Bondy, and Dr. Angela Lin got me interested in TS when...

...exogenous sequences and regenerates a native peptide bond at the split site. Figure 1: The base editor...

...cysteines, such as those that make up the disulfide bonds between antibody chains. 3) Endogenous carbohydrates...

...promoters were also tested in different plasmid backbones that varied in size or carried different genes...

...plasmid, 5-prime, CRISPR, growth strains, vector backbone, etc.) and you simply don't have enough context...

...CombiGEM-based assembly into the CombiGEM lentiviral vector backbone pAWp12 (Figure 2). Cloning from the pAWp28 storage...

...disrupted interactions between Cas9 and the phophate backbone of the target DNA strand via mutations N497A, ...

...things like butanol - very simple molecules - a few carbons and oxygens. Nothing too much more sophisticated...

...cloning my target spacers into the PX330 vector backbone? There is no need to add the NGG PAM sequence....designing oligos for cloning my target sequence into a backbone that uses the human U6 promoter to drive expression...plasmid having a targeting spacer cloned into the backbone ( pX330 or PX335 , depending if you want to use...quickly check if the cloning of my oligo into a backbone like PX330 worked? You can quickly check which...

...at the Faculty of Sciences of NOVA University in Lisbon, Portugal. She leads the Glycoimmunology group ...

...only occur when galactose replaces glucose as the carbon source. Student results have shown that most, but...

...gRNA expression can be achieved using lentiviral backbones expressing blasticidin or puromycin resistance...

...reader-effector proteins. b) Golden Gate assembly (backbones available at Addgene) with examples of potential...

...(about 2.7kb total)—is inserted into a plasmid backbone for delivery to the cells. As a precautionary ...

...Encodes one insert, encodes gRNA/shRNA, or empty backbone. Purpose Answer “What does this plasmid ...

...it’s also a good place to look for publications. Bonus: papers have to be summarized in 280 characters!...

.../10.1038/s41598-019-41088-2 Magnus C, Lee P, Bonaventura J, Zemla R, Gomez J, Ramirez M, Hu X, Galvan ...

... make your own Fragmid components! Ensure your backbone and genes contain only the Bbsl cut sites needed...

...100–115 mM = 0.7%–0.8% (v/v) Breaks disulfide bonds HCl Adjust pH to 6.7–6.8 Adjust pH ...