...Biosafety Blog: Tips for Titering Your Lentiviral Preps Lentiviral Pooled Libraries All Viral Plasmids Packaging...Expresses shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included to monitor expression Parijs...Expresses shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included to monitor expression Kissler...transgene and CMV drives expression of RFP as a reporter. See plasmid 17618 for GFP plasmid. Cheng 17452...

...populations. For more information, see the bioRxiv preprint describing ICE . Deep Sequencing Data Analysis...non-homologous end joining (NHEJ) and homologous directed repair (HDR) frequency. CRISPResso requires two inputs...for genome editing, as well as gene regulation (repression and activation). Genome support for bacteria ... lab: Tips for CRISPR design, homology directed repair and cloning . CRISPR Discussion Group (moderated...

... engineered small proteins derived from ankyrin repeat proteins. These are antibody mimics instead of ...isotype. Overview of application Fix your samples. Prepare your samples using permeabilization, antigen retrieval...and controls follow that of IF (above). Sample preparation, signaling excitation, and assay readout vary...must also be run. Unique samples (non-biological replicates) often each require their own set of controls...conjugating them in the lab? How will the sample be prepped, and will this or other factors affect epitope ...

...bound to DNA, and the break can be repaired by the cell’s endogenous repair mechanisms. Cas3 belongs to the...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Cas3...

...and innovation programme and EFPIA. Figure 1: Representation of RESOLUTE mission. The combination of metabolomics...deorphanizing engine. Genetic and proteomic interactions represent the framework that provides biological context...the Resolute Partnership using their RESOLUTE repository feedback form . If you have further questions...

...Serotype Testing AAV These AAV encode fluorescent reporters and can be used to compare the tropism of different...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...

...Endogenous Zebrafish Gene Targets Recent published reports have shown that engineered zinc finger nucleases...greater in a well-characterized bacterial two-hybrid reporter system but have NOT yet been tested for activity...OZ552 OPEN OPEN gGTCATCTGCcattcGAGGCGGACa REST corepressor 2 OZ553 and OZ554 OPEN OPEN gCTCACCATCtgttggaTGCGATGGAa...

...elegans genome is well studied and hundreds of reporters have been developed and characterized which allow...plasmid tools for the community that aren't in the repository? Help us widen our resources for the community...Contains a set of plasmids for building homologous repair templates that incorporate a self-excising drug...

...164499 EZH2-mEGFP AICSDP-83 mEGFP EZH2 Polycomb repressive complex 2 164500 POLR2A-mEGFP AICSDP-117 mEGFP...II 168798 TERF2-mEGFP AICSDP-77 mEGFP Telomeric repeat-binding factor 2 (TRF2) Telomeres 168799 CTCF-mEGFP...Proliferating cell nuclear antigen (PCNA) DNA replication foci IPSC cell lines can be ordered from the ...

...array of research purposes. Ready-to-use viral preparations for select plasmids are now available through...of certain genes that are required for viral replication. Thus, as a safety feature, viruses used in research...Instead, the genes that are required for viral replication are usually divided among many plasmids, such...

...Plasmids ID Plasmid Description PI Viral Preps ID Viral Prep Description PI (Cargo) PI (Capsid) 214869... and network function. Browse plasmids or viral preps created with support from the BRAIN Armamentarium...

...efficiency. These plasmids use the inverted terminal repeats (ITR) from AAV serotype 2. SaCas9 only: This plasmid... in p53 and Lkb1 , as well as homology directed repair-mediated Kras G12D mutations, leading to macroscopic...plasmid also contains a Kras G12D homology directed repair donor template. #60225 - AAV:ITR-U6-sgRNA(LacZ)...

...Plasmid Collections Depositor Collections Addgene's repository contains 156,671 plasmids, which includes plasmid...Synthetic Biology Voigt Fragmented T7 RNAP Plasmids Repressor Plasmids Sigma (σ) Factor Plasmids Terminator ...

...Collections Addgene Plasmid Collections Addgene's repository contains 156,671 plasmids . We've gathered our...looking for a non-plasmid item, browse our entire repository . Plasmid Resources Our curated resources group...

... terms. Looking for viral preps? We also offer ready-to-use AAV preparations of many chemogenetics plasmids...

... terms. Looking for viral preps? We also offer ready-to-use AAV preparations of many optogenetics plasmids...

...Addgene's Viral Service : Request ready-to-use viral preps of select CRISPR lentiviral plasmids. CRISPR Plasmid...expression. Interfere dCas9 alone, or fused to a repressor peptide, inhibits transcription. Epigenetics dCas9...

...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...sequences within exons, whereas CRISPR activation or repression experiments use targets within promoters. Do ...

...cloning grade DNA order? For each cloning grade DNA prep, the plasmid sequence has been validated by next...guarantee that Addgene will make cloning grade DNA preps of this plasmid available, but helps to inform us...

...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...