...plasmid from 2016 is an expression vector for CRISPR nuclease, SpCas9 from the Zhang Lab. It's name, though ...restriction sites into which you can clone your 20 nucleotide guide sequence of choice (for generation of a...

...2011, from zinc finger nucleases to transcription activator-like effector nucleases (TALENs). Our most popular...

...Soup by Aliyah Weinstein RNA aptamers, which are nucleic acid sequences that fluoresce upon binding a small...Transfection by Alyssa Cecchetelli The introduction of nucleic acids into bacteria or eukaryotic cells is a common...

...40822 EGFP-alphasynuclein-WT SNCA GFP CMV Parkinson's David Rubinsztein 40823 EGFP-alphasynuclein-A53T SNCA... pHM6-alphasynuclein-WT SNCA His, HA CMV Parkinson's David Rubinsztein 40825 pHM6-alphasynuclein-A53T ... Alpha-synuclein SNCA T7 Parkinson's Michael J Fox Foundation MJFF 51486 pET21a-alpha-synuclein SNCA T7...Alpha-synuclein SNCA T7 Parkinson's Michael J Fox Foundation MJFF 89074 pD454-SR rat Alpha-synuclein SNCA...Alpha-synuclein SNCA T7 Parkinson's Michael J Fox Foundation MJFF 89076 pD434-SR rat Alpha-synuclein SNCA...89470 VN-alpha Synuclein SNCA Venus CMV Parkinson's Bradley Hyman 89471 alpha Synuclein-VC SNCA Venus ...89483 VN-beta Synuclein SNCB Venus CMV Lewy body dementia Tiago Outeiro 89484 beta Synuclein-VC SNCB Venus...

...addition of 4-hydroxytamoxifen, it can move to the nucleus for activity. They also developed one of these ...gene activation and editing. Read the paper in Nucleic Acids Research Find the drug inducible Cas9 plasmids...

...repressor Mxi1, and an optogenetic tool that controls nuclear localization (LINuS). Blue light renders the NLS...NLS accessible and the complex enters the nucleus where dCas9-Mxi1 can repress gene expression. In the...

...and binds the inverted repeats (green); and an endonuclease reaction occurs which cuts the DNA.3.) The released...transposon can now bind a strand of DNA with a TA dinucleotide (there are many such sites in the human genome...

...engineering from ZFNs through TALENs and CRISPR-Cas nucleases, provided important context for the rest of the...described a chimeric Cas9 that is improving the nuclease specificity dramatically and reducing off-target...

...green cloud) functions both as a restriction endonuclease and as a ligase by cleaving and rejoining supercoiled... 2. Novel approach to molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase....

...tertiary structure when transcribed which aids in nuclear export of mRNA and helps increase the expression...The polyadenylation or polyA signal aids in the nuclear export of RNA and RNA translation, and promotes...

...adeno-associated virus particles containing complementary polynucleotide chains. J Virol 9(2): 394-396. Lecomte E, Tournaire...Next-generation Sequencing. Molecular Therapy - Nucleic Acids 4:e260 . https://doi.org/10.1038/mtna.2015.32...

... IgnaviCas9 is a hyperthermostable Cas9 CRISPR nuclease that is active up to 100°C.  The IgnaviCas9 protein...for long time periods and workflows where CRISPR nuclease activity is desired and time or protocol steps...

...catalytically dead Cas9 protein (dCas9) that lacks endonuclease activity coupled with a customizable single ...RNA (scRNA) will bind to a PAM-adjacent target nucleotide sequence then subsequently recruit a transcriptional...

...chromosomes and how these arrangements may affect nuclear processes. The latest of these visualization tools...the gRNA in living cells. Broccoli aptamers are nucleic acid sequences that bind to the small molecule,...

...contain other cellular molecules such as proteins or nucleic acids. This conjunction of vectors and exosome ...contain cellular macromolecules (e.g. proteins, nucleic acids) which possibly improve transduction properties...

...incorporation of a 3’-5’ exonuclease in the reaction mixture. The exonuclease removes mismatched 3’ overhangs...

... and whether there are any other contaminants. Nucleic acids (DNA and RNA) absorb maximally at 260 nm....to spectrophotometric methods that measure all nucleic acids). These methods are more sensitive than UV...

...and has previously been used for multiplex Cas9-nuclease editing. Typically, a single array encoding the...editing, such as base editing, prime editing, and nuclease editing, in virtually any cell type or organisms...

....com. ITR structure  ITRs are symmetrical 145-nucleotide sequences that flank the ends of the single stranded...Figure 2). Additionally, the ITR contains a 4-nucleotide Rep binding element (RBE) that serves as a binding...

... JM110 or INV110. Avoid star activity. Some endonucleases (for example BamHI) are capable of cleaving ...manufacturers make High Fidelity versions of the endonucleases and/or supply custom buffers as means to avoid...