...our newsletter or on our hot plasmids webpage. Listen to the entire Hot Plasmids podcast! Hot plasmids...transgenic lines to study neurodegenerative diseases. Listen to Our Segement on the Tol 2 Gateway-Compatible...AAV Vectors for tageting GABA-ergic interneurons. Listen to Our Segment on these new AAV Vectors Hot Plasmid...monomeric, and cyan-excitable red fluorescent protein. Listen to Our Segment on mCyRFP1 Hot Plasmid 4: mScarlet... bright, and monmeric red fluorescent protein.  Listen to Our Segment on mScarlet Special thanks to our... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer....     Additional Resources on the Addgene Blog Listen to Our Podcast Interview with Eye Researcher Connie...

...Multiple first or corresponding author publications (published, not in preparation) A history of successful funding...A project with sufficient preliminary data to establish your own lab and get an independent investigator...the beginning of Year 5. Now work backwards. To publish a submitted manuscript in a high-profile journal...typically have 4-5 years to make discoveries and publish papers to position yourself for a job search. Therefore...the best possible outcome, e.g. where could you publish this and how important is the result? Is there... much new data, speculate on what you hope to accomplish and how your research fits in with the current... meet future collaborators and get access to unpublished information and reagents. Part of the reason ...

...communication activities, e.g. tweet it when you publish a new blog post, and live tweet from conferences... If you live in the UK, join the SciComm mailing list. 7) Internships – Learn on the job Nothing prepares...internship at a magazine or newspaper (here’s a partial list). A great opportunity is the AAAS Mass Media Fellowship...into science communication by taking a media or journalism course. Courses can help you gain work experience...like Northwestern University or UC Berkeley have journalism graduate schools where you may take a science...research and residential communication. If science journalism is the path you want to follow, check out the... at the National Council for the Training of Journalists (NCTJ) or the Alan Alda Center for Communicating...

...introduced into the B. subtilis genome. Mobile-CRISPRi is then transferred from B. subtilis to the bacterium...colonies, but some bacteria, including E. faecalis and P. mirabilis, had only ~1 transfer per 1,000,000 colonies...CRISPRi into a single bacterial strain, such as a B. subtilis, or a narrow range of bacterial species, such ...integrative and conjugative elements in Bacillus subtilis (ICEBs1) system. ICEBs1 carries the genes needed...bi-parental mating. Following excision from the B. subtilis genome, the Mobile-CRISPRi ICE element forms a...integrative and conjugative element in Bacillus subtilis." Plasmid 86 (2016): 14-25. PubMed PMID: 27381852...

...the information. In contrast, a list of experiments or a laundry list of molecules to screen has unclear...Either way, think realistically about the time and resources required to feasibly accomplish the aims and ...proteins regulate the Z pathway?" is an open-ended list of proteins. Instead, consider "how do proteins ...exist to solve the problem. What will you have accomplished in 5-10 years? You need to be able to describe...learned and/or now be possible to do? Research accomplishments are remembered for the problem solved, not ...chalk talk with questions from the committee. For a list of common questions, check out this eBook about ...

...complicated! Listen to the full podcast for more great science communication tips or listen to the chapters...topics discussed during the interview. Happy listening! Listen to the interview with Science in the News ...Pint series. Chapter 4: The SITN Podcast, Sit'N Listen - In their podcast, the SITN team brings in scientists...like to develop the SITN podcast as a means for listeners to understand the dynamics of information flow... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer...

...stupendous listener, but I aspire to  be. If you don’t at least aspire to be a good listener and consciously...brought to the table was empathy, a willingness to listen, and an ability to remember the right stories at...disagreeable, you recognize their feelings as valid. Listening: In one of my favorite books, Michael Ende’s Momo... nearby village (and to the reader) by simply listening. Chances are, you know someone like this, even...Ende, courtesy of morefamousquotes.com A good listener absorbs and remembers and makes the teller feel...collaboration may not be the easiest resolution to accomplish, but there is a potential for long-term gains...examples of times when you have used your empathy, listening skills, and institutional knowledge to resolve...

...annurev-neuro-111020-100834 Challis, R.C., Ravindra Kumar, S., Chan, K.Y., Challis, C., Beadle, K., Jang, ...or lumbar spine. Image and caption courtesy of Challis et al., 2022. Where do they come from? Systemic...Addgene’s Data Hub and the CLOVER repository of published systemic AAVs to find commonly used vector cargo...capsid, while Table 1 gives a brief overview of published descriptions of commonly used or emerging capsids...https://clover.caltech.edu. Figure adapted from Challis et al. 2022. Image created with BioRender.com. ... plasmids available from Addgene Capsid Published Tropism Model Organism Mouse Strain-Specific...Strain-Specific Tropism?" column. For a complete list of mice strains with LY6A-dependent tropism, see...

...citations in its first four years (1). In addition to publishing in Science, Professor Zhang deposited the associated...CRISPR/Cas papers and Addgene deposits, but papers published in other years also get many more citations This...For example, it is very plausible that science published in more widely-read journals gets more citations...adjust our estimates based on the journal that published the articles.  That is, we re-estimate the citations...associated with Addgene deposits as if they were published in the same journals as those that did make deposits...papers with Addgene plasmids in our data were published in Nature, but only 3 percent of papers without... Weighting Example Article Number (A) Year Published (B) Number of Citations (C) Journal Name (D) Percent...

...and why other publishers and organizations have various different versions of checklists and guidelines...communication falls on the shoulders of journals and publishers. As journal editors, we often find ourselves ...contributions, etc.) before their paper can be published. To a scientist, all of this checking and formatting...communication further up the collective priority list. There has been progress—for example, a number of... of other Cell Press journals, including Cell Metabolism, Cell Reports and Joule. In September 2017 she...reproducible Read all of our blog posts on scientific publishing  ...

...Imaging Improving Well-established High-throughput assays Improving Well-established Biological sources...firefly(Photinus pyralis) 560 61 Luciferin ATP No Japanese firefly(Luciola cruciate/lateralis)Italian firefly...organism for most people is the firefly (Photinus pyralis) and perhaps not surprisingly it is also the most...describing the cloning of firefly luciferase was published in 1985, several studies utilized luciferase as...luciferase isolated from the firefly beetle (Photinus pyralis) is the most frequently used bioluminescent reporter...

...for controlling molecular processes with light. Listen to Our Segement on PhoCl Segment  Hot Plasmid 2...selection for increased CRISPR editing efficiency. Listen to the Segement on Ouabain Selection  Hot Plasmid...base editors with enhanced range and specificity. Listen to to the Segmennt of Improved CRISPR Base Editors...Plasmids for generating DNA molecular weight markers.  Listen to the Segement on the pPSU Plasmids Special thanks... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer....     Additional Resources on the Addgene Blog Listen to Our Podcast Interview with Eye Researcher Connie...

...cells). pBACKBONE-XXXXX Tip: For a catalog of published and commercially available empty backbones, visit...fusions that are on your insert. Typically you would list any tag or fusion protein in the order they appear... tag on the N-terminal of your insert, you would list it first. pBACKBONE-Flag-hGene If there was also...fused to the C-terminal of your insert you would list it after the insert. pBACKBONE-Flag-hGene-EGFP Other...included in the plasmid name. Mutations are generally listed as the amino acid change and not a nucleotide change...proper way to denote an amino acid mutation is to list the one letter abbreviation of the wild type amino...

...Pasteurella multocida, Porphyromonas gingivalis, Proteus mirabilis, Providencia spp., Pseudomonas spp.,...stability, which influence the plasmid’s ability to establish and maintain itself within host cells.  The plasmid...bacteria in lab manipulations. Table 1 has a detailed list of the bacterial species these plasmids are compatible...compatible with.  Table 1: Non-exhaustive list of common broad host range plasmids and the bacterial species...Myxococcus virescens, M. xanthus, Proteus rettgeri, P. mirabilis, Providencia stuartii, Pseudomonas spp., Rhizobium...campestris, X. campestris pv. malvacearum, Zymomonas mobilis pBBR1 based pBBR1  Gram-negative  Alcaligenes...

...2013 there have been 33 CRISPR software tools published and documented by OMICtools. It must be confusing...The first of only three such databases, EENdb (published 1 Jan 2013, no longer available), is a simple ...sgRNA designs. The second database, CrisprGE (published 27 Jun 2015), is noteworthy for its broad scope... content, and ease of access. The third, WGE (published 15 Sep 2015), is more similar to EENdb than CrisprGE...in detail, but that is already covered in the published articles. Instead, I have broken down the tools...or another. Supported organisms: This section lists the genomes supported by each tool. The genomes ...agnostic and have currently available organisms listed in this section. CRISPR software advice for specific...

...conversation. Goals and Planning Worksheet: Lists accomplishments, research/professional goals and feedback...credit for what we have accomplished. Starting with a recap of accomplishments, from learning new techniques...time has gone. Are we any closer to graduating, publishing that key paper, or figuring out a career path...tangible research and career goals, and celebrate accomplishments. These techniques are laid out in Vincent et...

...Organizing your lab notebooks, protocols, and to do lists can be easier than you think! Here are three tips...the colors and text. Tip 2: Use electronic to-do lists to access your tasks on the go Never miss a deadline...everything to your calendar. Ditch the paper To Do List and move to one that moves with you. Use software...software such as Evernote or Google Tasks to create lists of your upcoming tasks, set deadlines, and arrange...attachments. Organize your notes into notebooks, create checklists that link to other notes, and use the search ...spreadsheets for reagent storage locations, such as the list used to organize your cryo freezer. No more issues...

...Jessica Polka from Harvard Medical School and a long list of supporters from all of the research centers in...problems resulting from past practices and mores.  His list is dire:  The current system is unsustainable, hypercompetition...be radically overhauled.  Finally, the science publishing system needs to be changed.  Dr. Bourne charged...training excellence to begin effecting change.  Panelists call for action Each of the speakers in the panel...applause when he talked about how he boycotts publishing in all but open access journals.  Dr. David Glass...astounding lack of reproducibility being recognized in published work even in the “best” journals.  He pushed for...

...we'd like to extend a special thank you to Dr. Tom Ellis (Imperial College London), Dr. Connie Cepko (Harvard...asked leading professors for their top 3 tips. Tom Ellis 1. Keep up with the literature. He recommends setting...too; both with your research and with getting published.” Connie Cepko 1. Work hard. There will be periods...mentees who have goals outside of the university? Ellis recommends that a student interested in leaving ...engineering (STEM) expertise in law, business, journalism, entertainment, politics, K-12, etc.” Addgene's...Acknowledgments Thank you again to Connie Cepko, Tom Ellis, and George Church for answering our burning questions...

...for Microbiology (ASM) Scientific Writing and Publishing Institute. Writing is the cornerstone of any ... them with the world. If one doesn’t write and publish in some way, then the data produced isn’t likely...manuscripts and the ASM Scientific Writing and Publishing Institute (SWPI) is an extremely beneficial guide...should possibly take a composition class in the English Department. He suggested that I sign up for the...the SWPI online course because a course in the English department wouldn’t have trained me in the scientific...Resources on the Addgene Blog Dos & Don'ts When Publishing a Scientific Manuscript Writing Scientific Manuscripts...