...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific...adenine to inosine, which is replaced by guanosine to create A->G (or T->C on the opposite strand) mutations...breaks in DNA instead of the double-strand breaks created by wild type Cas enzymes. To use a nickase mutant...your DNA in close proximity. These double nicks create a double-strand break (DSB) that is repaired using...fused to a transcriptional activator peptide can increase transcription of a specific gene. Design your ...

...factor isoform in pooled screens. This collection and library can be used for screening transcription factors...available at Addgene. The collection can be used for screening transcription factors for cellular programming....026 . TF ORF barcode PCR for NGS QC and bulk screening (85 KB) TF ORF barcode dial out PCR for 10X scRNA-seq...bp sequence, to simplify identification during screening. Library ID PI Description MORF library 137000...

...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific...breaks in DNA instead of the double-strand breaks created by wild type Cas enzymes. To use a nickase mutant...your DNA in close proximity. These double nicks create a double-strand break (DSB) that is repaired using...fused to a transcriptional activator peptide can increase transcription of a specific gene. Design your ...

...Return to top Secretory Pathway The secretory pathway is named for its role in secreting proteins from...Posts Background General Cytoskeletal Junctions Secretory Pathway Background The subcellular localization... shipped. Many of the proteins that enter the secretory pathway require chaperones to help with folding... or search the table below to find markers of secretory pathway components. ID Plasmid Structure Targeting...

...tetracycline-controlled transactivator (tTA) was developed. tTA was created by fusing tetR with the C-terminal domain of VP16...tTA binding to the tetO sequences and subsequent increase in expression by the activation domain, resulting...repressor, or rTetR, which reversed the phenotype and created a reliance on the presence of tetracycline for ... etracycline-controlled t rans a ctivator) was created by fusing rTetR with VP16. The tetracycline on ...interest with Strep-Tag and Tet-On 3G transactivator, creating an auto-regulated Tet-On 3G System. Contains homology...

...carcinoid, pancreatic, and related neuroendocrine cancers. The NETRF believes that creating and maintaining... accelerate scientific discovery that will help create new and more effective therapies for these tumors...States and Drivers in Intestinal Carcinoid and Pancreatic Neuroendocrine Tumors...

...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...breaks in DNA instead of the double-strand breaks created by wild type Cas enzymes. To use a nickase mutant...your DNA in close proximity. These double nicks create a double-strand break (DSB) that is repaired using...activity, experimental considerations, and more, created and maintained by the Doudna Lab . Browse, sort...

... for expressing secreted recombinant proteins from human erythrocytes that was created for systematic ...receptor proteins for use with AVEXIS interaction screening Special...systematic screening of extracellular protein interactions to identify novel receptor-ligand interactions that ...

...CRISPR plasmids that create double strand breaks in DNA....Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific...activity, experimental considerations, and more, created and maintained by the Doudna Lab . Browse, sort...

...antibodies targeting families of cell surface and secreted proteins from a collaboration between Addgene ...antibodies targeting families of cell surface and secreted proteins (glypicans and integrins) and common ...common epitope tags, as well as antibody plasmids to create or express plasmid-based antibodies. The majority... Plasmids The following plasmids can be used to create or express plasmid-based antibodies. ID Plasmid...

...activity. The variety in sequence decreases the gRNA activity and increases off-target effect. Which species...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...requirements, experimental considerations, and more, created and maintained by the Doudna Lab . gRNA Plasmids...

...expressing secreted recombinant proteins from human platelets that was created for systematic screening of extracellular...receptor proteins for use with AVEXIS interaction screening Special...

...next generation architecture has been shown to increase mutation induction when using TALENs. The FokI...Gersbach These new destination vectors can be used to create TALE-transcription factors and are used at the ...TALEN kit, (ii) a lacZ fragment for blue/white-screening in E.coli , (iii) CAG promoter and Kozak sequence...these plasmids along with the FLASH method can decrease the amount of Histone H3 lysine 4 (H3K4) di-methylation...

...list of EXPRESs plasmids used with AVEXIS for screening in zebrafish....collection of plasmids that was used for systematic screening of extracellular protein interactions to identify... references for more information: Large-scale screening for novel low-affinity extracellular protein interactions...

...antibodies to zebrafish cell surface receptors and secreted proteins....antibodies to zebrafish cell surface receptors and secreted proteins. The plasmids encode both the light and...transfecting mammalian cell lines and collecting the secreted monoclonal antibody from the supernatant. Please...

... a collection of plasmids that was created for systematic screening of extracellular protein interactions...Plasmodium vivax merozoite protein plasmids for screening protein interactions Special...

...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...adenosine deaminase to catalytically dead Cas13b creates a programmable RNA editor that converts adenosine...activity, experimental considerations, and more, created and maintained by the Doudna Lab . Browse, sort...

...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...fused to a transcriptional activator peptide can increase transcription of a specific gene. Design your ...activity, experimental considerations, and more, created and maintained by the Doudna Lab . Browse, sort...

...gene expression. Screen CRISPR libraries are a powerful tool for genome-wide screening. RNA Targeting gRNAs... a specific DNA site Transcriptional Regulation Screen Activate dCas9 fused to an activator peptide activates...

...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...to deletions generated by Cas9, large deletions created using Cas3 have fewer off-target alterations and... Figure 1: Overview of Cascade-Cas3 mechanism. Created with BioRender.com. Browse, sort, or search the...