...ophthalmology & visual science 59.3 (2018): 1630-1640. Sakaguchi, Richi, Marcus N. Leiwe, and Takeshi Imai. "Bright... Zebrabow animals to an inducible Cre line. The Schier group developed Zebrabow as a system that takes...optimizes Brainbow for current technologies Tetbow (Sakaguchi et al., 2018) overcame the low expression of XFP...such as the brain, transparent so that the 3D architecture of a fluorescent neuron is visible. Chemical...

...has been achieved with the Rinehart system (6); however different proteins will likely achieve different...suppression, as described (6). To determine the stoichiometry of phospho-incorporated to non-phospho-incorporated...-Sung, et al. "Expanding the genetic code of Escherichia coli with phosphoserine." Science 333.6046 (2011...al. "A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation...

...obvious that a desired career outcome is not easily achieved. Of note, it was highlighted during the conference...Positive Points for Trainees - Taking Steps to Achieve a Successful Career Career Advice from the Career...showing researchers that they can take steps to achieve success in their careers. The take-away message...research. As during previous panels, speakers Moon Duchin, Rafael Luna, Joan Reede, Alberto Roca, and Jessica...and stability of academic research. Panelist Moon Duchin provided a concrete example of how underrepresented...

...Mizutani E, Sato H, Kasai M, Ogawa A, Suchy F, Yamaguchi T, Nakauchi H (2018) Intra-embryo Gene Cassette Knockin...mouse embryos, the embryos allowed to develop into chimeric mice (with a fraction of the cells in the adult...adult mice derived from the engineered ESCs), and chimeric adults mated to produce completely transgenic ...Wang is a professor at the Institute of Zoology, Chinese Academy of Sciences, and he is interested in using...

...dual-nuclease Cas9-Cas9 chimeras Scot Wolfe’s lab has developed dual-nuclease Cas9-Cas9 chimeras with higher target... in Nature Communications Find the Cas9-Cas9 chimera plasmids A modular toolkit for genome editing...transcription factor localization and binding via ChiP-seq. Learn more about ENCODE Find the transcription...

... Many thanks to our guest blogger, Chinmaya Sandangi! Dr. Chinmaya Sadangi is currently a postdoc at the...This post was contributed by guest blogger Chinmaya Sadangi, a postdoc at the University of Toronto. ...goal of communicating science to non-scientists. Chinmaya Sadangi, a postdoc at the University of Toronto...

...well as archived seminar presentations. One of SITN's newest initiatives, DayCon was the brainchild of SITN...Currently in its 16th year, Science in the News has two chief goals. First, SITN works to bridge the communication...information about SITN: DayCon event website (includes archived content) Science in the News website Check our...

...tumors effectively in mice. Chimeric antigen receptors T cells producing chimeric antigen receptors (CARs)...expressing CD19 chimeric antigen receptors for acute lymphoblastic leukaemia in children and young adults...proteins involved in immune tolerance. Recently, China spearheaded the first ever CRISPR-based clinical...

...wonderful opportunity to once again reflect on our achievements in the last year and share the exciting things...distributor partners, with the launch of antibodies in China and expansion of viral services available in South...partially through work with our distributors in China, Korea, Japan, India, and Brazil. Through an expansion...companies, while making use of our nonprofit status to achieve mission-driven goals of accelerating research and...

...experiments, consider that wildtype Cas9 with optimized chimeric gRNA has high efficiency but has been shown to...which has comparable efficiency to the optimized chimeric design but with better accuracy (in other words...nickase system is that you can express two different chimeric gRNAs with the Cas9 nickase which will together...site with efficiency similar to using a single chimeric gRNA. At the same time, the off-target effects...

...another method Transfection and transduction seem to achieve similar outcomes, so why and when should you opt...Second, what is the biological outcome you want to achieve? For example, do you want the nucleic acid cargo...work is that you can swap out your virus type to achieve different biological outcomes. For example, some...targeting of that virus, which can’t be easily achieved with alternative methods. Ready, set, transduce...

...chromosomal DNA, but they still rely on the host machinery to make additional copies. ori sequences are generally...lower temperatures and allows the replication machinery room to come in and get busy making copies.   ...incompatible because they will compete for the same machinery, creating an unstable and unpredictable environment...multiple-unnatural amino acid mutagenesis in Escherichia coli. Biochemistry. 2013 Mar 12;52(10):1828-37...

....  You will find an extra layer of difficulty launching a company in the health sciences without those...guidance.  Before even beginning the process of launching a company, you need to determine whether or not...viable.  This is not to say that we aren’t still pitching for feedback.  Your venture should continuously...you know your idea, you will rarely succeed in launching a company without partners.  You need a dedicated...

...This post was contributed by Adam Chin-Fatt, a Ph.D. student at the University of Western Ontario. Adam...the overexpression of various metabolites in Escherichia coli or Saccharomyces cerevisiae with few techniques...programs. Thank you to our guest blogger! Adam Chin-Fatt is a Ph.D. student at the University of Western...recombinant pharmaceuticals. Follow him on Twitter @AdamChinFatt     References Qi, L. S. et al. Repurposing...

...Presentation Experience”, “Career Achievements”, and “Academic Achievements”. Leaning my CV more towards me...time in the lab, my colleagues and what I was researching, but I decided I needed a new challenge. Looking... business friendly CV that would focus on my achievements, capabilities, courses and office skills. I ...

...but not limited to biotechnology, chemistry, architecture, engineering, mathematics, physical sciences...should only focus on your current scientific achievements. Participating in the scientific review process...international recognition for your outstanding achievements in your specific academic field, (2) that you...receipt of major prizes or awards for outstanding achievement Evidence of membership in associations that require...require their members to demonstrate outstanding achievement Evidence of published material in professional...

... as many viral particles must infect a cell to achieve visible expression of the protein. However, expression...expression of receptors may not need to be as high to achieve an observable signal in your experiment, therefore...looking for effective gene expression. How do you achieve high titers in the first place? Some AAVs are known...solution. Add a bit less buffer than you would to achieve the exact desired concentration to avoid over-diluting...

...oligos can be cloned to create a chimeric guide RNA. Find pX330-U6-Chimeric_BB-CBh-hSpCas9. pSpCas9(BB)-2A-...years on their own or through Addgene!) pX330-U6-Chimeric_BB-CBh-hSpCas9 - The S. pyogenes Cas9 (SpCas9) has... This plasmid expresses the S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from the U6...

...thanks to our guest bloggers Joachim Goedhard and Marieke Mastop! Joachim Goedhart is an assistant professor...This post was contributed by guest bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular...fluorescent probes. You can follow him on twitter: @joachimgoedhart.   Marieke Mastop is a PhD candidate at the...

... need, and we wanted to support all of them — searching, browsing, drilling down, and zooming out — with...that best match a user’s query, and return the matching resources in an order that makes sense to the ...synapsin, hsyn synapsin-1, human synapsin, hSynap. Matching We capture multiple variations of the same information...ID). Depositor suggestions provide shortcuts to matching PI labs and institutions. There are many more ...