... address the growing clinical problem of antibiotic resistant bacteria. See the picture to the left for... drug resistantance genes in the bacteria thus making the bacteria sensitive to known antibotics. These... able to participate when the organizers of the Canton Nucleic Acids Forum (CNAF) 2015 conference invited...scientists still working at the bench. It was a fantastic opportunity to talk to so many scientists who ... of plasmid requests from Addgene. Addgene - Canton Nucleic Acids Forum 2015 from Joanne Kamens, PhD...mammals, expressed in neurons, and are especially abundant in neuronal synapses. shRNA silencing of one circular...his list of the bacterial species that “ESKAPE” antibiotic treatment resulting in major problems in hospitals...

...gene targets. To accomplish this they have taken advantage of the Zhang lab all-in-one (guide/Cas9/reporter... with EF1alpha addition of an mCherry reporter variant nuclear reporters via an H2B fusion shuttle plasmid...fusion for gene activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic of the CRISPR Collection. ...

...available as cgDNA, we hope to aid scientists who want to immediately start cloning upon arrival of their...determined by spectrophotometer. Other methods of quantification may yield slightly different DNA concentrations...Suggestion Form . Please note that this does not guarantee that Addgene will make cloning grade DNA preps...

... Medicines Initiative 2 Joint Undertaking under grant agreement No 777372. This Joint Undertaking receives... production is used for assays and to generate antibody/ high-affinity ligands. Data is embedded in an...knowledgebase that features, among others, genetic variants and disease associations. Plasmids This plasmid...

...-type malate dehydrogenase genes and watermelon mutant clones for your course-based undergraduate research...single protein with many available isoforms and mutants provides a shared experience for the research community...of wild-type MDH genes from several species and mutant clones from watermelon have been codon-optimized...

..., not enough protocols, cost, not enough good antibodies, access to reagents)? For All Attendees: To help...for this meeting's publication, we are asking participants to share practical information in the form of... practices (e.g., dose response data, list of antibodies and recommended dilutions, etc.), novel applications...

...See below for plasmid kits and other resources relevant to these organisms, or use our Search page to ...Creating three-part fusion proteins - Planas and Santalucia Labs pCri System : Heterologous protein expression...MoClo : Modular cloning for applications like recombinant protein purification and cell-free circuit design...

...though actual yields may vary by construct. Size Quantity Total Volume Minimum Titer Typical Titer Small... on Request preps is all-inclusive of several important steps: MTA facilitation for the transfer plasmid...you need more assistance with troubleshooting, we want to hear from you! Email us at [email protected] ....

...Jennifer Tsang. CRISPR-Cas technology is constantly evolving. Variants of Cas proteins can be used for genome...proteins. Discovery of anti-CRISPR proteins That is, until the discovery of anti-CRISPR proteins in 2012...DNA. Since these early anti-CRISPR studies, scientists have discovered over 50 anti-CRISPR proteins that...they block can be found here. How do anti-CRISPR proteins work? Anti-CRISPRs proteins are highly diverse...2012 (Bondy-Denomy et al., 2012). Anti-CRISPR (Acr) proteins are phage-derived small protein inhibitors...the Sontheimer and Davidson labs discovered three anti-CRISPRs for a type II system in Neisseria meningitidis...genome editing. In this study, the labs found that anti-CRISPR prevents Cas9 activity in HEK293 by reducing...

...effectors, which were isolated from the plant bacterial pathogen Xanthomonas. The central TAL targeting domain...plasmid toolbox. Addgene has rapidly become an important resource for this new technology, and we hope ...

...transcription activator-like (TAL) effectors of plant pathogenic Xanthomonas spp. to the FokI nuclease, TALENs bind...Guide CRISPR Plasmids Zinc Finger Kits TALENs are important new tools for genome engineering. Fusions of transcription...

...Plasmid grant this year. These scientists work on projects spanning from the identification of plant compounds... has worked with both animal and plant viruses. Now focusing on plant biology, she coordinates the Network...Cowpea, a knowledge database for this important agricultural plant. Her current research focuses on endophytes...reside within living plants and that produce compounds with various antimicrobial activities (1). She ...Optogenetic Tools Like pDawn  Making cassava plants resistant to cassava mosaic virus Dr. Fiona Robertson... to increase the potential for this drought tolerant plant, Dr. Robertson aims to make use of the CRISPR...technology to engineer plants that are able to destroy DNA viruses, similar to the anti-phage CRISPR effect...

...Couple that with a condition known to the plant community as ‘plant blindness’ - the tendency for students...resource or you want to introduce Arabidopsis to your class for the first time – we want to hear from you...classroom Engineering the Plant Genome Using CRISPR/Cas9 pSiM24: Simplifying Plant Genetic Engineering Resources...Addgene.org Find Plant Vectors at Addgene Find Simple Protocols for Introductory Lab Work MoClo Plants Parts Kit...Biological Resource Center and the Center for Applied Plant Sciences at The Ohio State University. Established...year. Introducing Arabidopsis Arabidopsis is an important model system for research and is used by scientists... to be more interested in studying animals than plants, and biology teachers’ preference for using animal...

...Bernach. Melissa Chiasson: Quantifying the abundance of thousands of protein variants simultaneously  With ...effects of sequence variants on abundance simultaneously called VAMP-seq, for Variant Abundance by Massively...the abundance of a protein variant library is monitored by fusing the variants to eGFP followed by an internal...the target of the anticoagulant drug warfarin. In past research, several VKOR variants have been found ...University of Canterbury where he studies how bacteria interact with each other and plant surfaces. His...individual’s genome. But when they find genetic variants, what does that mean? Previous research shows ...approximately 75% of all protein-coding pathogenic human variants are decreased in protein abundance, possibly due...

... viral prep format. Please note this does not guarantee viral vector packaging service, but lets us know...the best capsid for your cell type. First, do you want to target CNS or PNS? Then consider species...species, and then cell type. For CNS: For infant rhesus macaques: Broad tropism - AAV.MaCPNS1, AAV.MaCPNS2...

...contributed by Cary Valley  Listen to the anti-CRISPR podcast segment Anti-CRISPR (Acr) proteins potently inhibit...picking approach to select cells with variant characteristics they wanted, the team identified Archon1, a novel...CRISPR-Cas9 activity switching via a novel optogenetic variant of AcrIIA4.” CASANOVA responds to blue light quickly...indicator Archon Article contributed by Shreya Vedantam  Listen to the voltage indicator podcast segment...vectors (pUC57-L1L4 and pUC57-L3L2) that offer the advantage of using restriction digest cloning to introduce...for protein-protein interaction (PPI) studies in plants that rely on transient transfection systems using...Biotechniques 2012. PubMed PMID: 23066669.Hecker A et al, Plant Physiol 2015. PubMed PMID: 25971551. Discovery ...

...Calcium Sensor: GCaMP7c 105321 pGP-AAV-syn-jGCaMP7c variant 1513-WPRE Syn jGCaMP7c none Constitutive 1 Kim ...Kim 105322 pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE Syn jGCaMP7c none Cre 1 Kim Calcium Sensor: GCaMP6f... viral prep format. Please note this does not guarantee viral vector packaging service, but lets us know...

...Browse plasmids expressing genes relevant to Parkinson's disease created by the Michael J. Fox Foundation...created a number of plasmids for expression of variants of LRRK2 , SNCA , and other proteins of interest...

... gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources...Like nickase technology, this technique reduces unwanted off-target effects of CRISPR. Browse, sort, or...

...to generate and characterize single nucleotide variant lines in an isogenic cellular background. All cell...create cell lines with endogenously-tagged gene variants or to differentiate human iPSCs into other cell...