Deep Mutational Scanning with One Pot Saturation Mutagenesis
Type
Blog Post
...this. 2) When your gene of interest has a BbvCI site(s). If so, the nicking mutagenesis protocol will still...still work if the orientation of the BbvCI site(s) of the gene are in the same orientation as those in ...site directed mutagenesis to remove the BbvCI site(s).
Overall, the key features of the one-pot saturation...