We narrowed to 461 results for: %s
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Celebrate Open Data Day with Addgene’s AAV Data Hub!
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Help Us Name Blugene’s New Virus Friend
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Behind the scenes: Addgene’s new search engine and more
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It’s CRISPR Week at Addgene!
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Addgene’s Viral Service - Why Virus? Why Now?
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Addgene’s Top 10 Blog Posts from 2014
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London Calling - Addgene’s New Europe Office in London
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Addgene’s New Way to Help You Share Science
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Plasmids 101: Modular Cloning
TypeBlog Post...References Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S. A modular cloning system for standardized...transcribed together. They can express one or more gene(s), and typically begin with a promoter and end with... -
To Each HIS Own
TypeBlog Post...Natl Acad Sci U S A. 1991;88(20):8972-6. Moks T, Abrahrnsen L, Oesterlof B, Josephson S, Oestling M, Enfors...polyhistidine tag, alternative fusion tags were used, such as S. aureus’ Protein A (Moks 1987). These tags, however... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog Post...10.1016/j.neuron.2016.01.040 Takeda S, Kadowaki S, Haga T, Takaesu H, Mitaku S (2002) Identification of G protein-coupled... -
RNA Extraction Without A Kit
TypeBlog Post... for at least 4 hours. If your final RNA sample(s) are resuspended in water or TE buffer, store them...break down RNA upon cell lysis. In the late 1970’s, Chirgwin and colleagues showed that a strong protein...extraction. Researchers at the NIH in the mid 1980’s set out to develop a protocol that skipped the ultracentrifugation... -
RaPID Detection of RNA-protein Interactions
TypeBlog Post...compared head-to-head with the E. coli and BioID2 BirA*s in the context of RaPID for their ability to detect...proteins were then subtracted from Ramanathan et al.’s RaPID datasets. Applications of RaPID RaPID sounds..., Rao, D.S., Neela, P.H., Zarnegar, B.J., Mondal, S., Roth, J.G., Gai, H., Kovalski, J.R., Siprashvili...