...gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species...

...capabilities, in collaboration with researchers at Stanford University’s Department of Bioengineering, to ...

...transient transgene expression, as well as the substantial immune response induced by the viral capsid. ...

...receptors play a key role in development and homeostasis, and the receptors and their downstream genes...

...Plasmid Promoter Cloning Enzyme(s) Validated In Resistance Co-expressed Cas9 Depositing lab Cas9 species...

...and OZ548 OPEN OPEN gAGCTCCGTCtcgctgGCGGCCGAAg histamine receptor H3 (hrh3) OZ549 and OZ550 OPEN OPEN gCGCCACGGAcaataaaGAGGACTGCa...

...speckles 159744 DMD-mEGFP AICSDP-55 mEGFP Dystrophin Costameres 159745 TFAM-mEGFP AICSDP-72 mEGFP Mitochondrial...

... invasion of the human erythrocyte by the blood stage of the malaria parasite, P. falciparum . Please ...

...to a reverse transcriptase (RT) is capable of installing targeted insertions, deletions, and all possible...

... panels) equipped with destabilized GFP calcineurin reporter (blue) and stained with PI (pink) to indicate...Image from Isaacman-Beck et al., 2019. Get started with sparse labeling Isaacman-Beck et al., bioRxiv...bioRxiv 2019. https://doi.org/10.1101/788679 A destabilized GFP reporter for calcineurin activity in yeast...transcriptional reporter consisting of a quickly-degraded destabilized GFP variant fused to a 4X CDRE repeat. In addition... that this method is compatible with dead cell staining using propidium iodine (PI), allowing for calcineurin...Truncated TALE-fluorescent protein fusions as a DNA staining dye Article contributed by Jennifer Tsang ...generated fluorescent protein fusions with tTALE to “stain” DNA. TALE proteins can bind specific DNA sequences...

...improved, truncated TALE backbone architecture as established by Miller et al. (PMID: 21179091), as well as...

... mouse, human, rat, yeast, frog, Brachypodium distachyon, Oryza sativa, Oryzias latipes CasFinder: Flexible...

... (Link opens in a new window) . As part of our standard viral production , these viral vectors undergo...

...their promising research projects.   Understanding drug resistance mechanisms of Mycobacterium tuberculosis...interested in understanding the mechanisms behind Mycobacterium tuberculosis resistance to pyrazinamide...targets. An early and accurate detection of PZA-resistance is necessary to improve tuberculosis care and...Sheen aims to identify new genes related to PZA-resistance by using different approaches, including CRISPR...Her research should provide us with a better understanding of PZA action and could potentially stimulate...diagnostic methods and improved therapies for PZA-resistant Mycobacteria tuberculosis. Investigation of multispecies...this condition are urgently needed. Therefore, understanding neuronal networks, their interactions and information...

...Promoter Project The Pleiades Promoter Project was established to advance research and therapies for brain disorders...

...169082 Knockout Mouse Turner N/A 10 13,500 Mouse Metastasis CRISPR-Cpf1(Cas12a) Double Knockout Library 123361...

... Biology. 2001. PubMed . MicroRNA detection in prostate tumors by quantitative real-time PCR (qPCR). ...

...the cell’s internal framework that assists in sustaining structure and function. The cytoskeleton is primarily...

...provide AAV viral preps, includes listing of transfer status for viral preps....

...populations, an antibiotic resistance gene (i.e., a gene whose product confers resistance to ampicillin) is included...efficiently in a laboratory setting. Stable - Plasmids are stable long-term either as purified DNA or ...dideoxy sequencing. To understand Sanger sequencing, we first need to understand DNA replication. DNA is... GUA, GUG Start AUG* Stop UAG (amber), UGA (opal), UAA (ochre) *AUG is the most common start codon. Alternative...purposes of isolating one specific DNA sequence. This obstacle has been overcome by the field of recombinant ...providing a benefit to the host, such as antibiotic resistance. This benefit can be context-dependent, and thus...their use of studying and manipulating genes. For instance, most plasmids are replicated in E. coli and are...