... of the MCC community. They support educators interested in integrating MDH-related research into their...

... cellular recognition processes. Laboratories interested in these approaches have created this resource...

.... Want an adenoviral plasmid with your gene of interest? Search our database for adenoviral transfer plasmids...

...promoter or regulatory regions of your gene of interest. If the plasmid that you choose does not also ...

...plasmids. Want a retroviral plasmid with your gene of interest? Search our database for retroviral transfer plasmids...

...20-basepair complementary region to any gene of interest. Co-expression of dCas9 and sgRNA can efficiently...

...easily visualize products from an AgeI/EcoRI restriction digest) David Root 10879 pLKO.1 ‐ TRC control...

...can be used as empty backbones for your gene of interest for expression in plants. You can search the table...

...genome are every 500 bp. TAL effectors have some restrictions (for example, the target must start with a T...

...instead encode for a specific gene or other research interest. To produce viruses with alternate (non-virus-...

... novel AAV vector targets ON bipolar cells and restores visual function in a mouse model of complete congenital...

...as a nuclease and as an activator, but is more restricted in the sequences it can target. Cas9 proteins...

...cell lines. Features include (i) Esp3I (BsmBI) restriction sites for full compatibility with the Golden ...

...reads from PCR amplicons that cover your locus of interest and correspond to edited and non-edited cell populations...

...method, mouse strain, target cell type or tissue of interest, and model organism under study. *Many engineered...

...template, you could then: Confirm your HR by doing Restriction Fragment Length Polymorphism (RFLP) ( see Figure...

...including ITRs), though there is a great deal of interest and effort directed toward expanding this capacity...

...very low or nonexistent. Scientists continue to wrestle with the possibility of postnatal and germline ...

...of R, is capped, and proceeds through U5 and the rest of the provirus. 3' LTR in cis Terminates transcription...plasmid encoding a transgene, sgRNA, or shRNA of interest: The transgene sequence is flanked by long terminal...

...of R, is capped, and proceeds through U5 and the rest of the provirus. Third generation vectors use a ...containing only one restriction site? If a lentiviral transfer plasmid contains a single restriction site, one ...subcloning or appending compatible restriction sites onto the insert of interest using PCR. The process of subcloning... basically shuffling restriction sites between vectors until the gene of interest is flanked by sites ...consuming and easier to simply add restriction sites onto the insert of interest using PCR. This is accomplished...using primers that contain the restriction sites needed. Functional restriction sites must be a certain number...Lentiviral transfer plasmid encoding your insert of interest. The transgene sequence is flanked by long terminal...