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  1. Viral Production

    Type
    Collection
    ...isolating genomic DNA. This DNA is then used as a template to amplify a portion of the intended lentiviral...

  3. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...is an Addgene depositor. KSQ uses a CRISPR-Cas9 platform to systematically test the function of all 20,...also interested to see Addgene expand the sharing platform to other reagents in biology. Luk H. Vandenberghe...

  4. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ... Pre-warming your antibiotic-containing plate prior to plating your transformation may allow you to see...

  5. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...DNA – perfect for transforma­­­tion or as a PCR template. Some of the applications of the NEBuilder HiFi...plasmids can be directly transformed into E.coli for plating, screening and generation of sgRNA. Generation ...

  8. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...receptor), then the transducer could be a microscope, plate reader, or any other instrument which measured GFP...advantages over other mechanisms. For one, many viral platforms, such as AAV or retrovirus, can either permanently...

  11. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...repair, as there is no requirement for a repair template (sister chromatid or homologue) or extensive DNA...NHEJ-dependent repair (“pop-in” insertion) provided a template containing compatible overhangs is available. ...

  12. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ... not limited to: rapid growth, ease of replica plating and mutant isolation, a well-defined genetic system...complementation is weak. yes   LYS2 L-lysine yes - Grow on plates containing alpha-aminoadipate in the absence of...

  14. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...relevant host biomarkers. The lab validated their platform on clinical stool samples by comparison to qPCR... al. "A low-cost paper-based synthetic biology platform for analyzing gut microbiota and host biomarkers...

  15. The time and cost required to make a plasmid

    Type
    Blog Post
    ...reagents and consumables, especially for things like plates and gels, which either don’t scale or have limited...use every single well in a 96-well (or 384-well) plate! How much do repositories help?   Designing precise...

  16. Hot Plasmids - October 2020

    Type
    Blog Post
    ...requires the much less efficient double stranded templates, rather than the more efficient single-stranded...cellular protein. They used CRISPR with a ssODN template to introduce the small wrmScarlet11 which is less...

  19. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...percent of cases, the stem cell will seek out a template for the repair process. By flooding the cell with...using the introduced donor plasmid as a repair template. This results in the insertion of the GFP tag ...

  20. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...vectors that each contains a guide RNA and repair template of your choosing (Schwartz and Jorgensen, 2016...PMC3531189. Mangone, Marco, et al. "UTRome. org: a platform for 3′ UTR biology in C. elegans." Nucleic acids...
Showing: 141 - 160 of 349 results