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  1. Meet the New Addgene Board Members!

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    Blog Post
    ...is an Addgene depositor. KSQ uses a CRISPR-Cas9 platform to systematically test the function of all 20,...also interested to see Addgene expand the sharing platform to other reagents in biology. Luk H. Vandenberghe...

  3. Plasmids 101: TOPO Cloning

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    Blog Post
    ... Pre-warming your antibiotic-containing plate prior to plating your transformation may allow you to see...

  4. Pushing the Limits of DNA Assembly

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    Blog Post
    ...DNA – perfect for transforma­­­tion or as a PCR template. Some of the applications of the NEBuilder HiFi...plasmids can be directly transformed into E.coli for plating, screening and generation of sgRNA. Generation ...

  8. Viral Vectors 101: Biosensors

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    Blog Post
    ...receptor), then the transducer could be a microscope, plate reader, or any other instrument which measured GFP...advantages over other mechanisms. For one, many viral platforms, such as AAV or retrovirus, can either permanently...

  9. CRISPR 101: Non-Homologous End Joining

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    Blog Post
    ...repair, as there is no requirement for a repair template (sister chromatid or homologue) or extensive DNA...NHEJ-dependent repair (“pop-in” insertion) provided a template containing compatible overhangs is available. ...

  10. Plasmids 101: Yeast Vectors

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    Blog Post
    ... not limited to: rapid growth, ease of replica plating and mutant isolation, a well-defined genetic system...complementation is weak. yes   LYS2 L-lysine yes - Grow on plates containing alpha-aminoadipate in the absence of...

  11. Plasmid Tools for Microbiome Studies

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    Blog Post
    ...relevant host biomarkers. The lab validated their platform on clinical stool samples by comparison to qPCR... al. "A low-cost paper-based synthetic biology platform for analyzing gut microbiota and host biomarkers...

  12. Plasmids for Endogenous Gene Tagging in Human Cells

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    Blog Post
    ...percent of cases, the stem cell will seek out a template for the repair process. By flooding the cell with...using the introduced donor plasmid as a repair template. This results in the insertion of the GFP tag ...

  13. The time and cost required to make a plasmid

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    Blog Post
    ...reagents and consumables, especially for things like plates and gels, which either don’t scale or have limited...use every single well in a 96-well (or 384-well) plate! How much do repositories help?   Designing precise...

  16. Open Resources and Plasmid Tools For Studying C. elegans

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    Blog Post
    ...vectors that each contains a guide RNA and repair template of your choosing (Schwartz and Jorgensen, 2016...PMC3531189. Mangone, Marco, et al. "UTRome. org: a platform for 3′ UTR biology in C. elegans." Nucleic acids...

  17. Hot Plasmids - October 2020

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    Blog Post
    ...requires the much less efficient double stranded templates, rather than the more efficient single-stranded...cellular protein. They used CRISPR with a ssODN template to introduce the small wrmScarlet11 which is less...

  19. 5 Tips for Troubleshooting Viral Transductions

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    Blog Post
    ...typically used (e.g., use 5 mL medium on a 10 cm plate). By reducing the culture volume into which the ...vector using the harvested viral solution as a PCR template. Regardless of the type of virus you’re working...

  20. Experimenting in an Open Source lab: from CRISPR to Cats

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    Blog Post
    ... have members looking at how to re-use it as a platform for monitoring homemade microfluidic chips using...using the fantastic optics and fluidics platform it contains. Biotech equipment becomes obsolete amazingly...
Showing: 141 - 160 of 341 results