...However, when I enquired about roles in this field, most jobs needed at least 3 months experience working... practicalities of working in the field with the most basic equipment and was a tremendously positive ... that all commercial companies are like this, as most have a more stable funding situation and level of... of work than research or academia, and so most positions in industry are more stable than this. However...experience is that the technology is so advanced that most of the procedures in the lab are done with robots... the phone or explain how various plasmids work. Most people who call or email me are scientists who are...self-motivated, because you need to work independently most of the time and plan your own work schedule.  I ...

... this here and now. Our closure was earlier than most companies. Since the start of this crisis, we’ve...mobilized on COVID-19 research, cures, and diagnostics. Almost immediately we began to receive urgent requests...guidance. We were also dealing with pending orders, but most labs around the world were closed and not receiving...challenge was our Finance Team. With Addgene closed for almost two weeks and with labs worldwide closing for unknown...Protection Program loan and received enough funds to make most of our payroll for the spring and early summer months...distributes and fulfills requests for scientists doing almost every type of biomedical science research. Our ...

...the C terminal of the tag. Of the most common tags, FLAG is the most charged, with 5 negatively charged...and applications. In this blog, we will review the most common tags, the antibodies used to detect them,...you access to a variety of antibodies that have almost certainly already been optimized for your application...specific monoclonal antibodies. Below we review the most commonly available tags and their antibodies along...antibody to Myc was developed in 1985 and is still the most prevalent clone used today! Goat, chicken, mouse...

...and is found mostly in blood. IgD While IgD is coexpressed with IgM on the surface of most immature B cells...blood, where it’s the second most common antibody after IgG; but it's the most prevalent antibody in secretions...valency of two and has no subclasses. IgG IgG is the most abundant isotype in blood, but it’s also found in...where it protects mucosal membranes. This isotype is most commonly a dimer with a valency of four. There are...

... proteins, etc., that you are interested in. The most obvious component of a biosensor is the thing that... culture and animal models primarily. Fun fact: Almost all of the biosensors discussed are genetically... Within these ions, calcium is by far one of the most popular, with dozens of sensors developed for it...potentials; it’s an ion of interest for many disciplines! Most of the sensors designed for calcium generate a fluorescent...intracellular, extracellular, and even the ADP:ATP ratio. Most of these sensors are fluorescence-based and have...tips. Still worried? We make virus with some of our most popular biosensors already packaged and ready to...

... next steps in the process involve repairing chromosomal DSBs. It is important to know that cells possess...extensive DNA synthesis. NHEJ also finishes repair of most types of breaks in tens of minutes – an order of...should possess indels in the majority of their chromosomes within a day. Another factor expected to impact...or more can simultaneously introduce a pair of chromosome breaks, and could result in deletion of the intervening...Stark JM (2009) Limiting the Persistence of a Chromosome Break Diminishes Its Mutagenic Potential. PLoS...EA, Giovannangeli C, Jasin M, Brunet E (2014) Chromosomal Translocations in Human Cells Are Generated by...

...stream to commute to and invade multiple organs, and most importantly escape the immune mechanisms of the ...known as the tumor microenvironment - a vital atmosphere created by the cancer cells to shield themselves...cell-surface tolerance-producing proteins are one of the most commonly used immunotherapeutic agents. Currently...receptors (CARs) on their surfaces have been the most acclaimed form of immunotherapy in recent times.... validation to identify populations that are the most likely to be positively impacted by the trial. During...potential beneficiaries. Collaborative therapy For the most efficient outcomes, collaborative therapies must...questions in this exciting field of research. One of the most important: How will cancers evolve and mutate based...

...Harvard Medical School working on human artificial chromosomes. Montreal, QC, Canada 45.5017°N 73.5673°W – Vanessa...to full reproductive health care. And it’s needed most in low-income and minority communities, where these... hardest. According to the Guttmacher Institute, most women of reproductive age live in a state with at...about my work because it's important to our health.  Most people won't argue with that idea, but they might...into a successful PhD thesis. We use the same atmospheric physics to understand exoplanets that we use ...organized the march was, as well as the friendly atmosphere that was created by the many thousands of science...science, but I worried (and continue to worry) that most who participated will consider that marching in ...

...Microscopy Fluorescent Widefield Microscopy is the most common and simplest form of fluorescent microscopy...developed that allow for sub-diffraction limit imaging. Most often, these techniques provide a 2-10x improvement...interference from blurry out-of-focus light. The most difficult characteristic to capture in these types...resolution on the millisecond scale may be needed. Most commonly these experiments are performed on a fluorescent... a fast, sensitive detector such as a scientific CMOS camera. If plasma membrane dynamics are the main...small model embryos >15 μm Thsee are some of the most challenging samples to image as they require both...quality images for this type of sample as they are most efficient at excluding out of focus light. However...

...efficiency than in iPSCs, but with the caveat of mosaicism. To circumvent this problem, they co-injected ...Indeed, they observed that 41/42 embryos were non-mosaic at 3 days post fertilization. Since Ma et al. designed...term ‘editing.’ While repair using endogenous chromosomal DNA is appealing for heterozygous mutations, ...needed to define whether off-target editing or mosaicism would occur. Although Ma et al. looked at various...this detailed analysis is not possible with IVF, as most of the cells are needed for proper development. .... Ma et al.’s experiments showed a 3% mosaic rate, and this rate would need to be much, much lower before...

...email me with suggestions.  Most coaches have websites you can review and most create useful online content...and do not know where to look for a coach. Many (most?) graduate students and postdocs may feel they have... have limited funds to invest in this. However most coaches who focus on scientists are sensitive to this...effective and also help keep the coaching costs low. Most will work with you remotely (by phone, Skype or .... This larger shop might appeal to some although most scientist coaches can help with transitioning out...

... be the most common poster size, but this can vary as well.  Sections of your poster In almost all scientific...be as concise as possible, communicating only the most important results to your audience. With a poster...Adobe also offers free poster templates, although most are vertical.  Large central box. Another commonly... of this style is that it will display your data most prominently, making it the star of your presentation...navigating the poster session Find tips to make the most of your conference attendance Read all of our blog...

...fast-tracked development of diagnostics. Mostly transmitted by Aedes mosquitoes (aegypti and albopictus) and, aided...virus had received little attention and, for the most part, had been associated with low morbidity and...conducive to the spread of the virus by the Aedes mosquito (Messina et al, 2016). We quickly assembled a ...true team effort and many late nights, not unlike most research, but the urgent need for the test did catalyze...community when we needed additional expertise. Perhaps most striking is that there were seven institutions involved...

...preparation and day-of game plan will ensure you have the most beneficial scientific conference experience possible...your poster as a visual aid, not a crutch. Even the most prepared of us can find ourselves talking to our...navigate questions Answering questions is arguably the most difficult part of a poster session, but with a little...which posters you are interested in viewing. For most larger conferences, poster titles and abstracts ...to the session, so bookmark which posters are of most interest to you a few days in advance. If you don...website. Time permitting, you can go back to the most interesting and popular posters at the end of the...

...delivery and gene therapy and represent one of the most studied viruses. Adenoviruses are non-enveloped ...isogenic AdVs encoding TALENs targeting the same chromosomal region. The CRISPR/Cas9-derived RNA-guided nuclease-induced... will interact with sporadic double-stranded chromosomal DNA breaks “that always happen naturally.” These...Muscular Dystrophy, Alpha1-antitrypsin deficiency (most widely used so far) or for cancer gene therapy such...Current state and clinical perspectives. Biochemistry Moscow 81:700–708 . https://doi.org/10.1134/s0006297916070063...Ventura A (2014) In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system. Nature...

...retroviruses have been developed into some of the most widely used tools in molecular biology. “Retroviruses...of their more complicated genomes. Here, we will mostly use the gamma-retroviruses as a simple spokesmodel...molecular biologists working with vectors are, in most cases, interested in transferring genes to mammalian...double stranded DNA is integrated into the host chromosome, and this is where viral vectors and natural ...express. In the case of a natural virus, this new chromosomal locus is considered “proviral DNA” (Rowe et al...Resources on addgene.org Addgene's Lentiviral Guide Most popular lentiviral plasmids γ-Retrovirus Guide ...

...change, the safety of genetically modified organisms (GMOs), the absence of a link between vaccines and autism...Association for the Advancement of Science) believe GMOs are safe for human consumption in comparison to ...active research scientists believe climate change is mostly due to human activity while much less of the general... of the word ‘fact’. Policy must be based on our most accurate interpretation of the world, and this interpretation...the following subjects: Climate change Vaccines GMOs Transgender issues Reproductive health Immigration...paired with long-term science outreach, may have the most profound impact (15). Diversity and inclusion ...

... expect: Search bar The biggest change is to our most frequently used feature: the search bar. We’ve changed... easy-to-use depositing guide, with links to our most helpful depositing articles. If you’re depositing...Figure 2: The new deposit materials bar New menu Most common activities can be done straight from the ...educational resources page, and previews for our three most recent blog posts. Finally, you’ll find everyone...discover as you browse. You may find one of your most frequently used buttons stands out better on the...

... is the best studied and most commonly used serotype. Its genome is the most common choice for AAV pseudotyping...adeno-associated viral vectors, or rAAVs, can sound almost too good to be true! In a previous post, we covered...the genome, and the second number to the capsid. Most vectors provided by Addgene are pseudotypes using...from one another. Common serotypes We’ll cover the most commonly used serotypes in this post. However, tissue...our in-stock viral prep service.  AAV5 AAV5 is the most genetically distinct AAV. Though AAV5 is one of ...

...questions can elicit the most valuable insights you will get from the interaction. Most people like to help...help us learn about the nonacademic sphere where most of us will be working.  Check out Joanne's Reddit... lists of questions on the internet. To make the most of the opportunity, use a written list and take ...stories. Thanks to the Benjamin Franklin Effect, most people actually enjoy helping (just ignore the people...