...produced in abundance, making them useful for in vivo studies. These attributes make frog eggs some of the...physiological, or developmental characteristics that make them ideal for studying in a laboratory environment...Additionally, its status as a Biosafety Level 1 microbe makes it a perfect candidate for introductory teaching...Iannaccone et al., 2009). Rats are larger than mice making them more useful for certain studies that involve...axolotl has several attractive characteristics that make them an ideal model organism; they are relatively...genetics and developmental patterns are conserved, making them great models for human disease research. One... alike.  Arabidopsis has many of the traits that make an ideal model organism: quick development, a large...

...’s critical to make these decisions in the following order of importance: 1. First make decisions based... Then make decisions based on financial values. All too often, we see early investigators making unnecessary...step by step instructions on creating a budget, making decisions based on that budget, and negotiating...science will define your success. Therefore, it only makes sense to reverse engineer your budgetary needs from...you can eliminate from your budget. Every cut you make signifies that you've made a conscious effort to...dependent on a pathology microtome/cryostat, then making a careful decision on the condition and availability...

... studies, making a meta analysis available to the community on ReFigure. ReFigure also makes it easier...during this period occurs infrequently and rarely makes it into the small selection of data found in full-length...early career researchers, we wondered how we could make the insights gained during this time more visible... on science. ReFigure saves time, knowledge and makes your insights discoverable. Watch this quick video...cell lines/model systems ReFigure allows you to make meaningful collections from a large section of open...paper they’re reading has been linked in a ReFigure making it easy for others to discover your insights on...

...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...with the cells’ endogenous ubiquitin ligase, the Kanemaki lab was able to translate AID to yeast, as well...Nishimura et al., 2009, Natsume et al., 2016).  The Kanemaki lab previously developed a CRISPR/Cas9 based method...essential proteins (Natsume et al., 2016). Now, the Kanemaki lab has created a new toolkit of plasmids for ...Yesbolatova et al., 2019) using CRISPR. This toolkit makes it easy to tag your protein of interest to create...create conditional human cell lines. Lastly, the Kanemaki lab demonstrated that auxinole, a TIR1 inhibitor...such as a distal promoter and an enhancer. What makes LADL unique from previous iterations is the addition...

...culture towards a more generous and rewarding way of making science.   How we started Crowdfight-COVID19...Confirm the expert’s suitability and availability; 3) Make the contact to start a direct collaboration between...realization that there is a great untapped potential to make science more efficient, open, and collaborative....collaborative. We now want to use this potential to make a difference not just against COVID-19, but also in other...These are transient partnerships where a researcher makes a small but crucial contribution to someone else...We are a community of researchers who believe in making science less of an individual effort and more of...bono. If this sounds appealing, join us and help make science a better place. You will receive opportunities...

...rushing, you’re much more likely to make a mistake. When you make a mistake, you’re more likely to be ...and the same goes for your workflow. Next time you make a mistake, instead of silently vowing never to do... physical copy of the protocol when you work and make a checkmark next to each step as you complete it...experimental steps are worth doing, and doing well, so make sure you’re taking time to do them carefully and...reach for spreadsheets, printouts, and even label makers to keep things legible. It is faster to spend more...however, many ways for you to reduce the chances of making an error. Try a variety of things, including (and...

...Alzheimer's - a connection I wouldn’t have thought to make (even if science writer Ed Yong’s book “I Contain... skills you’ve developed in your current work to make new insights in neuroscience. Even if you're currently...expertise and can be difficult for individual labs to make efficiently. For select viral vectors in the repository...repository, you can instead have Addgene make the virus for you. Neuroscientists are particularly interested...experiments. This helped reinforce our goals of making more AAVs and educational content to help researchers...for future experiments and to find tools that will make your transition into the field much easier! If there... suggest a plasmid to us, request that we start making the AAV, or help us produce new educational content...

...as a great first-time mentoring experience. They make mentoring less mysterious and train the participants...Provides a good mechanism for accountability that makes mentoring effective Goal setting is easier with ...example, a group of people who are all interested in making a transition. Schedule your first meeting and get...regularly no matter what, even if one person can’t make it. One of the biggest group mentoring predictors...breakfast once a month. All participants must commit to making the meetings a scheduling priority (and last minute... Set concrete goals with input from the group to make the plan better. Track your goal progress as a group...the series to get more advice on mentoring: What Makes a Good Mentor? Choosing a Good Mentor for Scientists...

...during your current job and note what makes your heart sing, what makes you pull your hair out, your successes...opportunities to pivot and grow along the way. That’s what makes it so wonderful. The most challenging part of jumping... in a government setting: drafting legislation, making rules, and implementing the policy within the jurisdiction...career or a part-time passion, there are many ways to make it work for your time and interests.  Action Items... healthcare systems so I could understand how to make new treatments and technologies accessible to patients...healthcare system? Defining your why is what will make you a competitive candidate for any science policy...your interest to future positions, but will also make your transition from academia to the policy world...

...of the Cas9 nuclease (PDB ID: 4OO8 and 4UN3), Slaymaker et al. hypothesized that Cas9 cutting efficiency...enough to keep off-target sites from being cut. Slaymaker et al reasoned that, if they decreased the positive...the electropositivity of the HNH/RuvC groove, Slaymaker et al. made a variety of alanine substitutions...off-target effects genome-wide. Further work by Slaymaker et al. showed that these mutants are not toxic...SpCas9-HF1, and HypaCas9 should enable researchers to make precise edits in mammalian cells and may decrease...look forward to seeing how the research community makes use of these tools and are excited to see continued...improvements in genome engineering! References 1. Slaymaker, Ian M., et al. "Rationally engineered Cas9 nucleases...

...technology and synthetic biology, the tools he’s making available through Addgene, and where all of it ...CRISPR is so orthogonal and programmable that you can make a very large number of regulators. That basically...have the solution. Our paper shows we can easily make orthogonal variation. The first one worked in each...off-target interactions. That’s relevant if you want to make sure you don’t target anywhere else, but for synthetic... been putting together very large toolboxes and making them available. We’ll also be submitting parts ...you want. The last few years, we’ve really been making tons of parts and characterizing the software to...sophisticated as those that living cells and animals make. That will be an outcome of genetic engineering....

...few things you can do to make the experience less stressful. First, you can make arrangements with those...company’s area of expertise. This has potential to make your audience really sit up and pay attention. Your...demonstrate technical and transferable skills that make you qualified for the position. Highlight the outcomes...improving a process or procedure, solving a problem, or making a key decision. Even deciding to stop pursuing ...4.5 hours, which was a lot of extraversion for me. Make sure to get enough sleep the night before. Have ... look professional  First impressions matter, so make sure yours counts. Dress professionally but still...

..., need to stay active and involved if we wish to make positive change. Below I present perspectives on...science happens when scientists have 1) the courage to make mistakes and 2) the integrity to recognize these...scientific evidence. We need representatives and policymakers who not only believe in the separation of church...-two-new-sciences). This is the approach policy makers take in abusing climate science skepticism to promote...averting a potential climate disaster. However, policy makers use these two fingers of skepticism to defend an...world. Justin Sherman is a Scientific Instrument Maker at Intellectual Ventures Laboratory. Vancouver, ...ongoing and difficult job. I urge other marchers to make this their first step, and continue this work by...

...Promoter Regions There are three main portions that make up a promoter: core promoter, proximal promoter,...

...tips on how to make a successful event at your company. The point of this post isn’t to make you all jealous...are busy with many other work responsibilities, we make team building activities a priority and have organized...though we are hiring!) but to encourage everyone to make an effort to build relationships with their colleagues... it simple This may be an obvious one, but don’t make things too complicated for yourself (especially ...for help My grandma used the old adage “many hands make light work” all the time. She was a wise lady for...will save you time and sanity!    10. Communicate Make sure people know that there is something fun happening...

...Gootenberg JS, Konermann S, Joung J, Slaymaker IM, Cox DBT, Shmakov S, Makarova KS, Semenova E, Minakhin L, Severinov...://doi.org/10.1261/rna.054098.115 Shmakov S, Abudayyeh OO, Makarova KS, Wolf YI, Gootenberg JS, Semenova...Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J,...

.... Scientists can make a new line of flies in only 6 weeks compared to months to make a transgenic mouse...in the lab, and have short generation times that make it easy to study the effects of genetic manipulations... be easily manipulated with tools like CRISPR to make transgenic lines. The fruit fly (Drosophila melanogaster...genetic material (Jennings, 2011).   The fruit fly makes a great model organism because they are easy and...C. elegans are also self-fertile hermaphrodites making it easy to conduct large experiments over multiple...60% of human genes have an ortholog in C. elegans making it ideal to study basic molecular biology processes...

...between different projects, you might not be able to make any reasonable progress on any one of them. That...another. Monitoring task progress. For this, I always make sure I have explicit goals in mind for the endpoints...everything in a Word or google doc that I can comment and make notes on as I progress. I’ve also recently started...world, procrastination can easily prevent you from making progress on a task. For instance, even if I had...over the activation energy barrier that comes with making ANY progress, the task will likely be much easier...easier. Having a formal plan for your day will make this easier - if you know you have dinner plans at 6...pm. Hopefully you’ll find this post useful for making your days, experiments, and projects more manageable...

... grow quickly and make more of the plasmids as they grow, scientists can easily make large amounts of ...guidance to understand the specific components that make up a plasmid and why each is important. Our “Plasmids...ability of plasmids to self-replicate within a cell make them attractive tools for the life scientist or ...particular environment Produce enzymes that will make specific, controlled changes to an organism’s genome...contains multiple protocols and troubleshooting tips to make plasmid usage as simple and straightforward as possible...

...their tropism, or the types of cells they infect, making AAV a very useful system for preferentially transducing...