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Showing: 141 - 160 of 955 results
  1. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting ...
  2. AAV Viral Preps

    Type
    Collection
    ...Tetracycline Transactivators, Affinity Purification, Cell Ablation Monosynaptic Neuronal Tracing Rabies Virus-Based...
  3. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting ...
  4. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting ...
  5. Antibody Plasmid Collection

    Type
    Collection
    ...of the pComb3 phage display vector system for isolation of scFv, diabody and Fab fragment libraries from...
  6. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...acceptor molecules and can therefore be used to calculate the distances between the donor and acceptor. ...
  7. Church Lab CRISPR Plasmids

    Type
    Collection
    ...Orthogonal Cas9 proteins for RNA-guided genome regulation and editing. Esvelt KM, Mali P, Braff JL, Moosburner...
  8. Chemogenetics Plasmids

    Type
    Collection
    ...to interrogate brain function and examine the relationship between neuronal signaling and behavior. Depending...
  9. Optogenetics AAV Preps

    Type
    Collection
    ...Promoter CAG CaMKII CBA Dlx EF1a/nEF Synapsin E2 regulatory element Fluorophore GFP Red-wavelength fluorescent...
  10. Biosensor AAV Preps

    Type
    Collection
    ...CAG CaMKIIa Dlx EF1a GFAP/GfaABC1D Synapsin E2 regulatory element Activity Cre-dependent Flp-dependent ...
  11. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ... Ca2+ channel phosphorylation site pS1928 [L132/18R] Cav1.2 Ca2+ channel phosphorylation site pS1928 Rat...
  12. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...Understanding intracellular pH regulation is important as pH regulation has roles in many cellular functions...the stop codon. Thus a non-translated mRNA will appear yellow, but a translated mRNA will be red. This system... were untranslated in the nucleus, confirming the hypothesis that most mRNAs are not translated until ...P-bodies are translationally repressed whereas the nonsequestered pool goes on to initiate translation. These...in separate subpopulations of mitochondria, one can monitor how the two separate populations move and interact...histidine kinase, which triggers phosphorylation of the response regulator and downstream transcription activation...particularly CRISPR), it is now relatively easy to design constructs and repair templates to insert TAP tag fusions...
  13. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ...perpendicular lines that intersect your populations. If you see a diagonal population, this is a sign of spillover... this case, the population on the left is a horizontal line, so our circled population on the right needs... bleed in this population. Note that in the corrected flow plot in 2A, the populations are not perfectly...straight, but the center of each population is in a line with the other populations.   Figure 2: Compensation... move it (Figure 3). If the population is not a double-positive population and is instead due to bleeding...for both at similar levels, meaning that your populations will ‘look’ similar on your flow plot. This will... difficult to properly gate your desired cell population. By compensating between fluorescent channels...
  14. Working with Nuclear Receptors

    Type
    Blog Post
    ...complexes with secondary coregulator (or co-coregulator) partners. The coregulator ‘core’ complex is composed...illnesses, and the metabolic syndrome. Coregulators Coregulators constitute a group of some 400 molecules... www.nursa.org for a curated list of coregulators). Coregulators can function as coactivators and corepressors...loosely bound co-coregulators associate with the core complex in a dynamic, regulated manner. The fact...individually contribute to cell regulation in a coherent manner. Because coregulators exist as multiprotein complexes...with a systematic effort to compile NR- and coregulator-related global gene profiling datasets in to our ... curated NRs and coregulators. We look forward to growing this important relationship with Addgene for...
Showing: 141 - 160 of 955 results