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Showing: 141 - 160 of 248 results

  1. CRISPR Plasmids - Tagging

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh - expresses Cas9, PITCh-gRNA, & FBL-specific gRNA pCRIS-PITChv2-FBL...containing Cas9 and a validated gRNA, based on the Zhang lab's PX458 . Multiple gRNA plasmids may be used. The...Plasmids Protein Species Tag Donor Plasmid gRNA plasmid gRNA plasmid FLAG pFETCh_Donor CEBPB Human FLAG...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR system...unavailable, you will need to design and clone in a gRNA(s) to guide the Cas9 protein to your target sequence...#79888) , which expresses an untagged Cas9 AND a gRNA targeting the AAVS1 locus. Doyon Tagging Plasmid...

  2. CRISPR Plasmids - Single-Strand Break (Nick)

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    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand...enzymes. To use a nickase mutant, you will need two gRNAs that target opposite strands of your DNA in close...

  3. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...find a gRNA vector based on expression system, promoter, the type of gRNA (e.g., pegRNA, epegRNA, nicking...Publication Empty Prime Editing gRNA Vectors A selection of empty gRNA vectors suitable for prime editing...using a prime editing guide RNA (pegRNA). As with a typical gRNA, the pegRNA is designed with a spacer that...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Prime editing is ...David Liu U6-pegRNA-H1-nick sgRNA-mCherry Mammalian, AAV hU6 + H1 pegRNA + nicking sgRNA No mCherry Hyongbum...PEmax to enhance pegRNA stability epegRNA - additional protection added to 3’ tail of pegRNA to prevent RNA...

  4. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts A catalytically inactive... break (DSB) at a specific sequence. Two unique gRNAs, binding ~15-25 bp apart, are required for dCas9...

  5. CRISPR Plasmids and Resources

    Type
    Collection
    ...Empty gRNA Vectors Select an empty vector for your experiment based on a variety of factors. gRNAs gRNA... : Browse depositor protocols, find software for gRNA design and deep sequencing analysis, discover links...powerful tool for genome-wide screening. RNA Targeting gRNAs RNA Targeting Type VI CRISPR systems target RNA,...nuclease generates a DSB when expressed with two gRNAs. Browse Plasmids by Model Organism Bacteria C. elegans...

  6. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Fully functional ...double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs are preferentially...flanking the DSB and 2. a specific edit close to the gRNA PAM site. When a repair template is present, the...

  7. Gersbach Lab CRISPR Plasmids

    Type
    Collection
    ...Plasmid 53186 phH1-gRNA : Expresses the S. pyogenes sgRNA from the H1 promoter. 53187 pmU6-gRNA : Expresses ...pyogenes sgRNA from the mouse U6 promoter. 53188 phU6-gRNA : Expresses the S. pyogenes sgRNA from the ... human U6 promoter. 53189 ph7SK-gRNA : Expresses the S. pyogenes sgRNA from the 7SK promoter. 53190 pLV...CRISPR Cas9 gRNA plasmids from Dr. Charles Gersbach's lab. CRISPR...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts These mammalian expression...target in gene promoters by the sgRNA expressed in trans from pSPgRNA, the dCas9-VP64 fusion protein activates...activates the expression of downstream genes. Multiple sgRNAs targeted to the same promoter act synergistically...

  8. CRISPR Guide

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    Collection
    ... of unique gRNAs. gRNA design for CRISPR libraries is usually optimized to select for gRNAs with high ... guide RNA ( gRNA or sgRNA ) and a CRISPR-associated endonuclease (Cas enzyme). The gRNA is a short synthetic...commonly referred to as an sgRNA. gRNA scaffold sequence The sequence within the gRNA that is responsible for...amplify a specific sequence of DNA pegRNA P rime E dit gRNA; a specialized gRNA containing an additional primer...Cas enzyme and a gRNA specific to the gene of interest. The genomic target of the gRNA can be any ~20 nucleotide...interactions between the gRNA scaffold and surface-exposed positively-charged grooves on Cas9. gRNA binding induces...if the gRNA spacer sequence shares sufficient homology with the target DNA. Once the Cas9-gRNA complex...

  9. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts To make targeted ... dead dCas9 to epigenetic modifiers. Design your gRNA to target a specific promoter or enhancer for your...

  10. CRISPR-Cas/RGN expression plasmids for human cells

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    Collection
    ...RNA ( gRNA ) expression vector listed below ( MLM3636 ) can be used to construct customized gRNAs targeted...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts This page lists plasmids...available, web-based ZiFiT Targeter software. Specific gRNA expression plasmids can be built by cloning a pair...the links below: ID Plasmid 43860 MLM3636 (human gRNA expression vector) 42252 MLM3639 (Cas9 expression...

  12. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...Addgene.org Browse Our CRISPR Resources Find Validated gRNAs Browse All Plasmids ...thousands of base pairs. Cloning sgRNAs Generating constructs to produce sgRNA for CRISPR experiments can be... plating, screening and generation of sgRNA. Generation of sgRNA libraries, with maximum complexity, is...constructs with multiple point mutations Cloning sgRNAs Production of Yeast Expression Cassettes Generating...synthetic oligo to generate a single-guide RNA (sgRNA). Using only one single-stranded oligo, instead ...to create a double-stranded oligo. Generating an sgRNA construct becomes as simple as combining your favorite...

  14. Guide RNA Expression Plasmids for EGFP

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    Collection
    ... the guide RNAs ( gRNAs ) used in these experiments are listed below. Note that gRNA produced from each... CRISPR Joung Lab CRISPR Plasmids EGFP gRNA CRISPR-Cas/RGN expression plasmids for EGFP You ...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The Joung Lab recently...

  15. Guide RNA Expression Plasmids for Endogenous Human Genes

    Type
    Collection
    ... the guide RNAs ( gRNAs ) used in these experiments are listed below. Note that gRNA produced from each... CRISPR Joung Lab CRISPR Plasmids Human gRNA CRISPR-Cas/RGN expression plasmids for Human genes...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The Joung Lab recently...

  16. CRISPR-Cas/RGN expression plasmids for Zebrafish

    Type
    Collection
    ...RNA ( gRNA ) expression vector listed below ( pDR274 ) can be used to construct customized gRNAs targeted...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts This page lists plasmids...available, web-based ZiFiT Targeter software. Specific gRNA expression plasmids can be built by cloning a pair...

  17. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...or Cas fused to a deaminase that makes the edit A gRNA targeting Cas to a specific locus A target base ...CRISPR Plasmids for Base Editing Find Validated gRNAs ...

  18. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ...Resources at Addgene Get Tips on How to Design a gRNA Read Our CRISPR Guide to Catch up on All the CRISPR...Read Other Blog Posts about CRISPR Find validated gRNAs ...of what determines good sgRNA design. Incidentally, the first software for sgRNA design (by Hsu et al.,...technical expertise, thoughtful single guide RNA (sgRNA) design, and the abnormally low GC content of the...without computational support. Indeed, rational sgRNA design is not possible without relying on some kind...end of 2014, I began developing software to make sgRNA design accessible to all. At the time I thought ...  Breaking down the barriers: My view on current sgRNA design tools The goal of this post is to provide...

  19. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type VI CRISPR systems...

  20. CRISPR Plasmids - Base Edit

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Two classes of base...Base editors are targeted to a specific locus by a gRNA, and they can convert cytidine to uridine within...
Showing: 141 - 160 of 248 results