...The most commonly used yeast by biotech and pharmaceutical companies is K. phaffii (formerly known as ...the system. Since then, multiple biotech and pharmaceutical companies (along with some research laboratories...It’s also a methylotropic yeast, meaning it can utilize methanol as its sole source of carbon and energy...distantly related to S. pombe and S. cerevisiae; evolutionarily it evolved much slower and bears more similarity...the expression of recombinant proteins by the pharmaceutical and biotech industries as a protein expression...greatest hurdle for genome engineering. A unique evolutionary feature of K. phaffii, relative to S. cerevisiae...

...display-based directed evolution to create APEX2. They have now applied the same directed evolution technique to...compartments is via proteomics--the study of the distribution and interactions of proteins within these structures...small-molecule substrate to map the spatiotemporal distribution and interaction networks of specific proteins...paper, they describe TurboID and miniTurbo, which utilize non-toxic biotin, but label nearly as quickly as...escape detection. The Alexander Green lab found a solution: a low-cost, cell-free detection assay that can...

...chemiluminescence, precipitate the protein out of solution, or isolate cells expressing this protein. Read... The hybridomas are then subcloned by limiting dilution, which produces an immortalized, clonal population... curve. This curve is generated using a serial dilution of a known amount of protein that is also detected...the protein of interest and pull it out of the solution. Once the unbound protein is washed away, the ...2014.   In vivo uses For both research and therapeutic purposes, antibodies can be used to affect cellular...functional grade antibodies. Some of the research and therapeutic uses overlap: antibodies can bind to and inhibit...investigating signaling pathways or developing therapeutics. Experimentally, antibodies conjugated to fluorescent...

... that present as global developmental delays or autism spectrum disorder. It is estimated that CTD is ...could greatly enhance our ability towards CTD therapeutic development,” says Iyer. Since the RESOLUTE plasmid...understand so that we could possibly develop pharmaceutical interventions that would inhibit IL-36 release...says Mellett. This would help the lab identify therapeutic targets in the future. Congratulations Mark Mellett...of developmental delay, and is characterized by autism-like symptoms, seizures, and muscle weakness. So...To study cellular transduction events, we will utilize BRET-, FRET-, and intensity-based signaling biosensors...

... gene expression. On the other hand, using a constitutive promoter in the same background resulted in ...over-represented compared to those further away. While constitutive promoters drove maximal gene expression near...during shifts in growth rate as compared to a constitutive promoter. To simulate the increase in plasmid...When they increased concentrations of IPTG, a constitutive promoter upstream of GFP increased expression...stabilized promoter while gene expression from the constitutive promoter did not. Similar levels of expression... al. placed the genes under the control of a constitutive promoter and then moved them into the genome...

...human conditions, opening up new avenues for therapeutics that treat disease at the molecular level. For...patients have achieved persistent immunological reconstitution with a normally functioning T cell repertoire...design to clinical trials involve identifying a therapeutic gene, finding a means to deliver it and identifying...issue by developing new capsids through direct evolution, capsid shuffling, and peptide displays. These... and other ongoing clinical trials support the utility of AAV as a broadly applicable gene delivery tool...understanding of disease pathogenesis will enable robust therapeutic outcomes. In contrast to other gene delivery ...

...can be removed either by filtration of the virus solution (through a 0.45 µm or 0.22 µm filter) or by a ...sterile PBS.  Alternatively, to concentrate a virus solution without ultracentrifugation, you can reduce the...the virus is released, a more concentrated virus solution can be obtained. 4. Enhancing virus-cell contact... in single-use aliquots. Never use a polybrene solution that has been thawed more than once. Fibronectin... packaging cells. This check can be performed routinely and without disrupting the flow of your experiment... of the viral vector using the harvested viral solution as a PCR template. Regardless of the type of virus...

...co-founders of Revolution Bioengineering.  Last year we started a company. Revolution Bioengineering ... as food but support GMOs that result in a new utility, was there a business opportunity out there for...changed color throughout the day. It would be a beautiful GMO, one that people would actually want to have...out of our careers: to build something new and beautiful, to engage with the public in a meaningful way... much knowledge and experience that goes into executing a successful business that the learning curve ... in the United States Air Force. She left active duty to pursue a degree in a synthetic biology laboratory...

...Creating xCas9 through directed evolution Hu et al. used phage-assisted evolution to generate and select for...of phage-assisted evolution, generating xCas9 2.0-2.6. This second round of evolution included continuous...lab, have gone one step further, using directed evolution to create xCas9, an enzyme recognizing a broad...broadest-targeting variants. For the final round of evolution, they used a no-G PAM library to enrich for Cas9...

...Collectively, the pool of CRISPR software embodies solutions that facilitate most experimental applications... engineering. When compared to that collective utility, it’s easy to fault the lack of features in a single... sgRNA design tools into database and de novo solutions. Database tools allow us to view and get a sense... workbenches such as MacVector or Benchling. Utility functions: These functions help speed up the sgRNA...single-method design”. Trends: The most common utility functions are batch design or multiplex features...repertoire. Organism agnostic tools are the better solution but are often packaged as offline tools requiring...use. The R based package CRISPRseek offers great utility and coupled with Bioconductor should be the first...

... DNA and proteins in solution. The pH is then lowered using a renaturing solution, which causes the proteins...genomic DNA to precipitate. Plasmid DNA is free in solution. Proteins and genomic DNA are removed by centrifugation...placed in a chaotropic salt sodium iodine (NaI) solution. NaI has two purposes in this protocol: 1) it ...: nucleic acids are broken out of cells with a solution of salts, detergent, and ball bearings. Then a...nucleic acids, so placing the dipstick into a wash solution removes contaminants while nucleic acids stick...

...create. Wildlife biology and ecology can make for beautifully compelling photos and videos, while molecular...platforms can splinter audience. Primarily digital distribution (though radio is possible.) Written works...works Low Flexible. Low barrier to entry. Ample distribution channels both digitally and physically. Easy...overwhelming your audience. Digital or print distribution.  Static. Can be difficult to convey an entire...highlighting, or omitting details. Digital or print distribution.  Static. Often requires both artistic and scientific...overlooked as a means of scicomm. Digital or print distribution.  Typically limited to a single page of information...

...refers to a saturated solution of formaldehyde (saturated being 37%). Formalin solutions often contain methanol... a fixative. However, when PFA is dissolved in solution and heated, it depolymerizes, producing formaldehyde...formaldehyde. Often people will call the resulting solution PFA, but it’s really just formaldehyde. Formalin...polymerization of formaldehyde.  Note: Formaldehyde in solution will naturally polymerize and lose its ability...the best results if you use fresh formaldehyde solutions.  Along with preserving your sample, cross-linking...

...bioinformatics at Addgene and how our search for solutions led us to a partnership with Seqera Labs.              ...automate plasmid sequence assembly, and possible solutions for each in the form of one-off scripts or programs...how effortless it was for us to switch between executing our pipeline on a local computer or in a cloud... each week!), we already planned to use cloud computing resources. Now, we could iterate our development...criteria that we needed. Prior to using Nextflow, we routinely wrote Python scripts, queried our database with...known as channels. Since a Nextflow process can utilize any scripting language that can be executed on ...

...2005-2023. We identified and offered a number of solutions that increased accessibility, such as using couriers...Beijing Zhongyuan 2012 China Summit Pharmaceuticals International 2013 Japan Leehyo Bioscience...country, and we can see a growth in material distribution after we partnered with them (Figure 2). In ...duration of their journey. Figure 2: Annual distribution of items to countries with a distributor. Arrows... some form of problem-solving. When universal solutions aren’t enough to ensure accessibility, partnering...formatting all 101s on a particular topic into beautifully designed and easy-to-reference eBooks. Figure...

...convergent evolution because electric organs arose in different branches of the evolutionary tree at least...freshman in college I studied development and evolution of histone genes in Strongylocentrotus purpuratus... and sperm, mix them in a tank, and observe the beauty of embryo development in real time. I was already...Breviolum minutum symbionts are important models for solutions to the coral reef crisis. I like how they are ...Chytrid fungi are useful for the study of basic evolution of fungi and eukaryotes and includes the fungus...environments so can be used study the genetic basis of evolution in immunology, neurobiology, and more. Prochlorococcus...

...get online and take a look at the company or institution’s career page, where you’re likely to find current...conference or job fair, or it may be someone at your institution’s career center. Alternatively, it might even ...other graduate students and post-docs from your institution have gone, and try sending them a professional... professional development, not count on your institution, program, or adviser to do it for you. Expose...for you to attend!), exploring a job fair, and utilizing your local career development center, if one exists... exists. Get online! Review company and institution career pages. Make yourself visible on LinkedIn and...

... range of dilutions of your lentiviral prep. Hopefully, by using more concentrated dilutions you will ... and poor transductions. Cell lines should be routinely subcultured at densities that are low enough to... tissue culture-based experiment, labs should routinely test for mycoplasma contamination. There are several...complete with freely available positive controls. Routinely test your cell line for mycoplasma and discard...lots of serum from the same source. Labs that routinely produce lentivirus may want to consider testing...

...phosphatase treatment, substituting cytidine with 5-methylcytidine, and/or substituting uridine with pseudouridine...this approach is still not considered safe for therapeutic applications. piggyBac (PB) transposon: The PB...integrate into the genome and are instead lost by dilution via cell division. A drawback to this approach...safer way to express reprogramming factors for therapeutic applications. Sendai viral vectors: Sendai virus...reporter. Minicircles are removed from cells by dilution with each cell division, but it can still take...is considered a safer approach for developing therapeutic applications, but it can take a long time to ...

...drawback for therapeutic applications where longer retention times often increase therapeutic efficacy (...better tissue penetration which is useful for therapeutic and imaging applications; and reduced immunogenicity... for cancer, autoimmune, and/or inflammatory therapeutic applications. Antibody fragments such as scFvs...fragments without disulfide bonds, made by molecular evolution 1 1Edited by I. A. Wilson. Journal of Molecular...  Sandomenico A, Sivaccumar JP, Ruvo M (2020) Evolution of Escherichia coli Expression System in Producing...