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...proteins, respectively. A plasmid's copy number has to do with the balance between positive and negative regulation...showing the standard features of a plasmid. So, how do you choose? Addgene Senior Scientist Marcy Patrick...have only one plasmid type in your cells at a time? Do you want to make a lot of your plasmid? Is the gene...for optimal results always pick a single colony and do not subculture directly from glycerol stocks, agar...

...given sequence. You want to choose enzymes that: Do not cut within your insert Are in the desired location...(usually in the Multiple Cloning Site (MCS)), but do not cut elsewhere on the plasmid Bonus: It is helpful...GAATTCATGTGGCATATCTCGAAGTAC-3'. Many restriction enzymes do not cut DNA efficiently at the end of a linear piece...any 6 bases, but you should ensure that the bases do not result in the formation of a hairpin structure...

...engineering of its function." Once scientists can do that, they will not only understand how the brain... neural basis of violence or aggression, it might do a lot to explain more difficult aspects of human ...decision making, to cure a human patient. We can't do it all by ourselves. Getting these tools out into...cells in arbitrary patterns in the brain. Addgene: Do you have any special tips or tricks for someone looking...

...Reactivity Recommended Applications PI Return to Top Do you have suggestions for other antibodies that should...

...Rett Syndrome Plasmid Collection and Resource Center Do you have suggestions for other plasmids that should...

...Optogenetics Content last reviewed: 25 September 2025 Do you have suggestions for other plasmids that should...

...Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you have suggestions for other plasmids that should...

...Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you have suggestions for other plasmids that should...

...Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you have suggestions for other plasmids that should...

...Packaging Service Content last reviewed: 4 December 2025 Do you have suggestions for other plasmids that should...

.... In the most practical terms, what GreenGate can do is “save you half a year,” in comparison to more ...sensitive protein,” he says. “There is nothing you can do about it but be gentle. If it no longer works, just...blocks will be available, making it easier for us to do our work.” References 1. Athanasios Lampropoulos...

...such as HEK and CHO do not seem to require this, while nearly all endocrine cells do. While not all of ...different densities, then dispose of the plates that do not appear to be yielding well-spaced clones. For...replicates of each clone in case of contamination. We do not normally use antibiotics. Lastly, and perhaps...experience, cell clones picked using the agarose method do not differ grossly from those isolated using cloning...

...will turn yellow. Do NOT change the media (add 2-3 mL of fresh media once a week), and do NOT harvest the...transgenes, transduce quiescent and dividing cells, and do not integrate into the host’s genome. The main challenge...test for the presence of RCA in the viral stock by doing a plaque formation assay on A549 cells . Alternatively...at -80C. Purification and concentration kits that do not use CsCl are commercially available. Titer calculation...

... easily separate out your target population. How do I know if I will need compensation? To know if you...on another.  What does spillover look like and how do I fix it? When plotting two channels against each... two channels. It is something your computer will do for you to better analyze your results; it does NOT...samples. With time and practice, you may be able to do your compensation setup on your experimental samples...compensation to your experimental samples In order to do compensation on your experimental samples, you will...

...that “negotiation” was something that I would mainly do over the price of a used car. Later, as I applied...the time over everything from which experiment to do next to how much data is necessary before submitting... someone can feed their cells this weekend - why? Do they have an important plans? What is most important...they different from mine? What equipment purchases do we prioritize in a lab with both electrophysiologists...help from another person/team at the company. How do I negotiate with the person/team for help? What can...

...fill this gap by both highlighting that researchers do learn MANY transferable skills while working in the...biostatistician, and that one golden-handed technician who can do a certain difficult assay with her eyes closed. How...career that I can’t help but feel a bit dubious when I do come across one. Also, remember, one important feature...80 hours a week in the lab, guess what? You are doing the work of more than one person. A selfish part...person’s name on your next paper, but you are not doing yourself or your science any favors by trying to...suffered for it. Nobody knew what anyone else was doing, and there were times when some members really should...of the team’s goals, then that team is destined to do poorly. To some extent, it doesn’t even matter whether...

...purification you can do is a miniprep, but if you need larger quantities of DNA, you’ll need to do a midiprep or...systems. Read on for an in-depth breakdown of how to do perform restriction digests.   Overview of...any pieces cut out or off of them. An easy way to do this is gel purification. In gel purification, you...empty plasmid, you will still have colonies when you do not add ligase. If the colonies are a result of recipient...significantly more colonies when you add ligase. If you do not see any colonies, you should conduct a positive...

... better equipped than anyone else in the world to do it.  In the lab, it might mean persuasively articulating...should change established theory. So, what can you do to ensure that your skills in science communication...presentation slides, learn from audiences as they do science outreach and engage with the public, and ...science communication or start your own! When you do, let us know!  To get in touch with ComSciCon, find...

...central We’re working on a project where we aim to do large scale DNA preps with multiple samples without... libraries as opposed to replicating them like we do at Addgene), going to the workshop was a great opportunity...What are the familiar parts of this workshop? What do I understand already? What don’t I understand? How...experience in my first blog post! I highly recommend doing the same if you ever go to a workshop because it...

...transduction coverage compared to just one vector.   Q2: Do you think it is possible to make an efficient AAV...method to be reliable and reproducible. However, we do not use amplicons in ITR as we have found this to...mice, e.g. first at 2 months, then at 4 months? Or do antibodies become a prohibitive problem? A: Re-administration... suppression to allow AAV re-administration but I do not think the field has settled on ‘best practices...titer by either method so to ensure equal animal dosing, we advise that users keep titering method consistent...vivo? A: This will be highly dependent on vector dose. The color diversity in Vector Assisted Spectral...insight into codelivery efficiency at different doses. See Figure 4 in this paper for more information...