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What's it like to do a master's degree in science communication? Guest blogger Alex Gilbert shares his experience.

The origin of replication is the DNA sequence which allows initiation of replication within a plasmid by recruiting transcriptional machinery proteins, enabling a plasmid to reproduce itself.

Learn all about BosLab, a DIY community science space in Somerville MA.

Addgene Executive Director provides advice on making the decision between academia and non-academia.

Get a quick overview of careers available to those with scientific training and the most pertinent skills for different types of careers.

PCR can be used to append the appropriate restriction sites to your insert and facilitate downstream cloning. Read on for tips on this process.

Interview with Ed Boyden discussing his newest optogenetics tools and the questions that motivate neuroengineering.

Sometimes flow machines have a hard time differientiating between colors - but a process called compensation can help. Learn more in our blog post!

Learn how to identify negotiation skills you already use in your lab and life and develop them further as a professional skill set.

Learn how to produce, purify, and titer adenoviral vectors for your research.

Quickly isolate mammalian cells clones using the agarose cloning method.

Tips to help you save time when using the the Basic Local Alignment Search Tool (BLAST) provided by NCBI to compare sequence results.

In this post from our Transferable Skills series, learn how teamwork can make both your lab life and your future career much easier.

The CRISPR babies saga shows us that we need action to move forward in bringing CRISPR technologies from bench to bedside.

Addgene lab technician Racheal Komuhendo describes her recent experiences at a workshop at Lab Central and how you can get the most out of a similar workshop.

Advice for scientists choosing a lab, including interviews with Principal Investigators and important factors to consider in your decision.

Read about how mammalian plasmids differ from their bacterial counterparts, including how replication occurs and whether selection is necessary for transfected cells.

The third post in the mentoring series, this post describes ways you can approach finding a science mentor and advice on how to “make the ask.”

Advice for things you can do to develop transferable skills while you are also learning to be an excellent scientist.

GreenGate is a cloning system for rapidly assembling plant transformation constructs and is based on the Golden Gate method.