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Showing: 141 - 160 of 981 results

  1. Individual REAL TALE Repeat Plasmids

    Type
    Collection
    ...recently described a rapid and robust protocol for constructing TALE repeat arrays of any desired sequence...Joung Lab REAL Assembly TALEN Kit , which also includes the four TALEN expression vectors. More detailed...

  2. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts To make targeted epigenetic...catalytically dead dCas9 to epigenetic modifiers. Design your gRNA to target a specific promoter or enhancer...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...

  3. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Catalytically dead dCas9...gene expression by interfering with transcription. Design your gRNA to target your gene of interest’s promoter...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...

  4. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Catalytically dead dCas9... can increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...

  5. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR/Cas nickase mutants...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...Selectable Marker PI Publication Yeast ID Plasmid Description Gene/Insert Promoter Selectable Marker PI Publication...

  6. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type VI CRISPR systems,...regardless of homology to the crRNA. This activity provides a stark contrast to Cas9 and Cpf1, which require...encyclopedia of CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...

  7. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Fully functional CRISPR...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...or search the tables below for CRISPR plasmids designed to introduce a DSB. Plasmids are available for...

  8. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR plasmids have been designed for use in ...CRISPR plasmids that have been designed for use in zebrafish. CRISPR...

  9. Optogenetics Plasmid Collection

    Type
    Collection
    ...many different types of proteins. For a full description (including peak response spectra) of different...preparations of many optogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter PI Publication...transcription", "Hahn", or other terms. ID Plasmid Description Gene/Insert Vector Type Promoter PI Publication...

  10. TALEN Engineering

    Type
    Collection
    ...laboratory for engineering TAL effectors, including designed TALENs, and assembly methods for REAL, REAL-Fast... engineering TAL effector repeat arrays of any desired length: the REAL (Restriction Enzyme And Ligation...for FLASH assembly. Links to additional pages describing these reagents are provided below: Joung Lab ...

  11. Resolute Plasmid Collection

    Type
    Collection
    ...proteomic interactions represent the framework that provides biological context. Assays monitoring impedance...sub-cloning into a wide range of Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry PI Pooled...solute carrier family of proteins. Library ID PI Description Human SLC Activation Library 132561 Superti-Furga...

  12. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...format and as a Lentiviral prep . ID Plasmid Description Mutations Industry PI Due to export control regulations...expressing mCherry and firefly luciferase. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro - Lentiviral...Lentiviral vector expressing destabilized EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter vector expressing...

  13. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Two classes of base editors...protein (Mu- GAM). While many base editors are designed to work in a very narrow window proximal to the...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations,...

  14. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...protein with many available isoforms and mutants provides a shared experience for the research community...site loop", or "subunit interface") ID Plasmid Description Gene/Insert Mutations Additional Resources MDH...Results in Student Learning Gains and Positive STEM Attitudes in CUREs. Callahan KP et al. CBE Life Sci Educ...

  15. Adenovirus Plasmids

    Type
    Collection
    ...strains for generating adenovirus. ID Strain Description PI 16398 BJ5183 Strain for recombination between... plasmids that have inserts. ID Plasmid Type Description PI 16400 pAdEasy-1 Adenoviral For recombining... be assembled in a one-tube reaction. ID Kit Description PI 1000000176 AdenoBuilder toolkit Plasmids contain...

  16. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...Mitochondria 87424 DSP-mEGFP AICSDP-9 mEGFP Desmoplakin Desmosomes 87425 ACTB-mEGFP AICSDP-15 mEGFP Beta-actin...human cell lines of their choice. For a full description of how to use these plasmids, check out Plasmids...

  17. Tetracycline Inducible Expression

    Type
    Collection
    ...tetracycline, tetracycline binds to tetR. This precludes tTA binding to the tetO sequences and subsequent...and induce your gene of interest. ID Plasmid Description Co-expressed tTA, rtTA, or TetR On or Off PI ...transactivator for your tetraycline system. ID Plasmid Description Element On or off PI 26429 pLenti CMV rtTA3 Blast...

  18. Optogenetics AAV Preps

    Type
    Collection
    ...ChrimsonR (soma-targeted) mRuby2 Cre dependent 9 Adesnik 124603 pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40... ChroME (soma-targeted) mRuby3 Cre dependent 9 Adesnik 124651 pAAV-CamKIIa-ChrimsonR-mScarlet-KV2.1 CaMKII...eGtACR1 (soma-targeted) mRuby3 Cre dependent 9 Adesnik 137155 pAAV-nEF-Con/Fon-iC++-EYFP nEF iC++ EYFP...

  19. Immunology Research Plasmids and Resources

    Type
    Collection
    ...pathogen. It includes physical barriers such as the skin and mucosa, antimicrobial peptides and proteins...adaptive immune response is antigen-specific and includes cellular and humoral immunity. Once an adaptive...activate immune components that also lead to the destruction of pathogens. Over the course of an infection...immune response. Over 60 interleukins have been described. NK Cell Signaling Natural killer (NK) cells can... NENF neuron derived neurotrophic factor CIR2, NEUDESIN, SCIRP10, SPUF NGF nerve growth factor (beta polypeptide...immune response. Over 60 interleukins have been described. Symbol Name Synonyms EBI3 Epstein-Barr Virus ...applications in mammalian systems. The MXS-chaining kit includes chaining vectors with multiple cloning sites and...

  20. Michael J Fox Foundation (MJFF) Plasmid Collection

    Type
    Collection
    ... Neurodegeneration Research Collection , which includes other areas of this research topic. Michael J....Browse or search the table below. ID Plasmid Description Gene/Insert Industry PI Return to top...
Showing: 141 - 160 of 981 results