... own nuclease activity. Instead, it forms a novel co-complex with the transposition protein TniQ for targeting...

...intimidating, but I had my lab’s protocols and some great co-workers to help me out. Even with these tools, I ...

...fragment, encode homology arms to allow immediate co-transformation with linearized vector backbone. ...

...expressing the inducer (i.e. tet-off transactivator) is co-injected. The dose of this AAV can be adjusted to...

...viral packaging and host genome integration. After co-transfection of these plasmids, 293T cells released...

...genes are each encoded on separate plasmids and are co-transfected into cells alongside a plasmid encoding...

...University of North Carolina at Chapel Hill. Under the co-mentorship Blossom Damania and Jack Griffith, her...

...work and make this happen. Be realistic and: Your co-workers will learn to work within these bounds You...

...pipette 200–1000 µL single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container...

...combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors. Jonathan...

...packaging capacity but necessitating purification and co-infection of two AAVs. Cas9 orthologs: shorter, ...

... General Meeting (now Microbe) in 2013 in Denver, CO. While I had been to the General Meeting seven other...

... fluorescence markers like GFP (510 nm), allowing co-imaging. And because bioluminescent markers do not...

... activation domains to the same genetic locus via co-expression of an epitope tagged dCas9 and antibody-activator...

...BbsI site. (c) The pCAG-EGxxFP target plasmid was co-transfected with pX330 plasmids containing sgRNA ...

... EF-1alpha driven expression of cDNA and and EGFP co-expression. See plasmid 21374 for dtomato version...

... 8E). In a 2-vector system, you’ll need to either co-infect with a Cas9-producing lentivirus or use a ...genes in mammalian cells include: SunTag system - co-expression of epitope-tagged dCas9 and antibody-activator...two-component system (Figure 9C) SAM activators - co-expression of dCas9-VP64 with a modified scaffold...non-repetitive genomic locus can also be labeled by co-delivering multiple gRNAs that tile the locus. Chromosome...dCas9 by fusing a biotin acceptor site to dCas9 and co-expressing BirA biotin ligase. Whatever the chosen...other Cas enzymes, the Cascade-Cas3 system has been co-opted for use in eukaryotic cells . The Cascade complex...

...induce your gene of interest. ID Plasmid Description Co-expressed tTA, rtTA, or TetR On or Off PI 21916 Tet-pLKO-neo...

...protocol, generates “clean” homozygous mutants with no co-integrated markers or footprints, and can be scaled...

... allow stronger counter-selection than one merely co-opted toward that aim (i.e. sacB). Importantly, to...