...scientists and non-scientists alike are marching for. I will be marching to advocate for science education ...is a Pro-Science and Pro-Evidence march. Who is marching and why? Who? All sorts of people are choosing...promises more cuts (3, 12, 13, 27). In order to achieve the breakthroughs, technologies, and treatments...exhaustive list and every marcher is not necessarily marching for every cause. The references provided also ...opinions or research on each topic. Resources for marching and advocacy If the march for science rallies ...

...Sommella A, Colecchi L, Rossi S, Simonelli F, Giunti M, Bacci ML, Polishchuk RS, Auricchio A (2015) Improved...genes into AAV First, some history. In 2008, the Auricchio lab decided to solve the issue of “tiny vector...tested and with the optimal sequences, the group achieved ~80% of muscle cells expressing mini-dystrophin...Cutillo L, Sparrow JR, Williams DS, Bennett J, Auricchio A (2008) Serotype-dependent packaging of large..., Giunti M, Palfi A, Farrar GJ, Polishchuk R, Auricchio A (2013) Effective delivery of large genes to ...

...typical PCR machines, the authors use affordable, field-ready, Bluetooth-enabled PCR machines that can even...approximately 1-2 hours Equipment needed: Mini PCR machines by DIY-Bio, P51 cardboard fluorescence visualizer...mentioned, likely ~1-2 hours Equipment needed: PCR machines/Heat blocks, Lateral flow strips, Agarose gel .../2020.04.07.028167 Broughton JP, Deng X, Yu G, Fasching CL, Servellita V, Singh J, Miao X, Streithorst...Miller S, Pan C-Y, Guevara H, Wadford DA, Chen JS, Chiu CY (2020) CRISPR–Cas12-based detection of SARS-CoV...

...Addgene Board of Directors. Cicalese served as the Chief Human Resource Officer for Harvard Medical School...Giving, an organization that raises money for childhood cancer through the sale of art. About two years...about Julie Cicalese  Ms. Cicalese served as the Chief Human Resources Officer for Harvard Medical School...Giving, a 501c3 public charity raising funds for childhood cancer through the sale of art. Past volunteer...Marathon for the MGH Pediatric Oncology and the Chicago Marathon to benefit Neurofibromatosis, New England...

...   Are you an only child and used to a lot of attention? Or are you one of 8 children and used to getting... with a new PI. I realized that as a first born child, I would thrive on this level of attention and interaction...role model Make sure the mentors you choose are teaching the stuff you want to know and display character... a mentor that sees you only as a paper making machine. Choose one that is a true mentor, supporting career...collaboration? If you are considering a career at a small, teaching college you might want to consider less expensive...

...understanding the brain’s function is knowing its architecture, in particular the connections between different...two pathways also use different cytoskeletal machineries to facilitate transport: retrograde transport...interaction with uptake or intracellular transport machinery. Addgene offers viral preparations of AAV2-retro...vectors. Improved neuronal tracing could also be achieved by using optogenetically controlled transgenes...neuromuscular diseases it would be desirable to achieve transgene delivery by targeting peripheral structures.... Beier, K.T., Saunders, A., Oldenburg, I.A., Miyamichi, K., Akhtar, N., Luo, L., Whelan, S.P., Sabatini...Kuleshova, E., Ojala, D., Huang, C.C., Gerfen, C.R., Schiller, J., Dudman, J.T., Hantman, A.W., Looger, L.L....

...be needed to achieve your intended next career stage and create a timeline for achieving these goals. ...the position, you may also want some evidence of teaching experience. How do you get there and more practically...not always available for mentees due to travel, teaching, grant writing, etc. Also, it is unlikely that...management and conflict resolution workshops. Get teaching experience and learn about modern pedagogy techniques...can help boost your ego in between scientific achievements. I've found cooking, gardening, hiking, and ...

...Below, you will find some easy talking points for broaching this subject with your institution’s tech transfer...resources (summaries, electronic MTA approval, archive of old MTAs, and more) Does your TTO have a question...of Medicine  Baylor College of Medicine Boston Children's Hospital  Brandeis University  Broad Institute...Laboratory  Dana-Farber Cancer Institute  Fred Hutchinson Cancer Research Center Memorial Sloan-Kettering...Studies The Scripps Research Institute St. Jude Children's Research Hospital  Stony Brook University  University...

...Travel Awards: Melissa Chiasson, Elizabeth Unger, and Michal Bernach. Melissa Chiasson: Quantifying the abundance...time is time consuming and unscalable.  Melissa Chiasson, a graduate student in Douglas Fowler's lab at...from each bin are then extracted and sequenced. Chiasson is currently using VAMP-seq to study vitamin K...scales and making personalized medicine a reality,” Chiasson explains. Elizabeth Unger: Developing a serotonin...

...Founded as a nonprofit in 2011, BioBuilder is the brainchild of Dr. Natalie Kuldell from MIT’s Department .... Built primarily from her 20 years of college teaching experience, Kuldell sought to create a program... begins with students reading comic strips and watching animations that present a specific research problem...test their own project ideas using synthetic biology.Launching with a kickoff meeting in October, BioBuilderClub...innovators that opened in August 2017.  This flexible teaching space offers STEM programming, workshops, and ...

... starting with an established method called ChIP-seq. ChIP-seq relies on antibodies to recognize and bind...been doing that for years using this assay called ChIP-seq, but it’s really hard for lots of transcription... antibody to any protein to get really beautiful ChIP-Seq results. The FLAG epitope is quite small—20 ...done comparisons of our new system versus standard ChIP-seq for transcription factors known to have good...

...conversions are base transversions: they require switching between a two-ring purine and one-ring pyrimidine...experimented with adding different proteins to the CGBE architecture. They conducted a CRISPRi screen to identify...were included in the CGBE. They also developed a machine learning model capable of predicting editing outcomes...gRNA/sgRNA Guide RNA RNA used by Cas9 to locate a matching DNA sequence.   Target strand The DNA strand... CRISPRi screens, target-library analysis, and machine learning. Nature Biotechnology, 39(11), 1414–1425...with sequence context preference predictable by machine learning methods. Nature Communications, 12(1),...

...Many thanks to our Guest Blogger Londa Schiebinger. Londa Schiebinger is a leading international expert on... post was contributed by guest blogger Londa Schiebinger, PhD, Hinds Professor of History of Science, ... stem cells. In this case, they found that sex-matching of donor and recipient yielded the best results...the recipient of numerous prizes and awards. Schiebinger addressed the United Nations on the topic of ...

...Guided by dCas9, VP64 recruits transcriptional machinery to specific sequences, causing targeted gene regulation...levels. Although other methods have been able to achieve much higher activation, dCas9-VP64 is great for...of interest, this allows more transcriptional machinery to be recruited per targeted gene. Performance...experiment! References Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA (2013) Programmable...Ewen-Campen B, Chari R, Ter-Ovanesyan D, Haque SJ, Cecchi RJ, Kowal EJK, Buchthal J, Housden BE, Perrimon...

... A., Ichiki, T., Mazmanian, S. K., Osborne, P. B., Keast, J. R., Miller, C. T., Fox, A. S., Chiu, I. M...the brain difficult. Traditionally, delivery is achieved through the use of invasive methods, limiting ...control of transgene expression or activity can be achieved using switches, including riboswitches and optogenetic...Introduction to AAV References Asencor, A. I., Dvoryanchikov, G., Makhoul, V., Tsoulfas, P., & Chaudhari,... W. R., Fish, K. N., & Pfenning, A. R. (2022). Machine learning sequence prioritization for cell type-...j.ymthe.2019.07.017 Matsuzaki, Y., Konno, A., Mochizuki, R., Shinohara, Y., Nitta, K., Okada, Y., & Hirai...Vormstein-Schneider, D., Lin, J. D., Pelkey, K. A., Chittajallu, R., Guo, B., Arias-Garcia, M. A., Allaway, K...

...partners, and/or children about everything from what you’re going to eat for dinner (frozen chicken fingers or...issue with childcare timing pickup. Whoever takes the earlier slot agrees to pick up both children while the...that the brand new grad student keeps using the machine? Let’s walk through a more specific example....

...smaller pieces, and proximity ligation creates chimeric circles of DNA that are distant linearly but close...“picture” is then developed by detecting these chimeric DNA fragments by PCR or next-generation sequencing... outperformed other 3D chromatin methods such as ChIA-PET or Hi-C, by increasing both the number of unique...on-target enrichment. 2. Determining the spatial and hierarchical organization of chromatin interaction networks...CAPTURE 2.0 is the construction of spatial and hierarchical chromatin interaction networks. Think of these...

...Filter By…” options changed when we changed from searching for viral preps instead of plasmids? The available... use to find materials from this publication. Searching for the title of a paper works You can also find...search results to exactly what you need: Better searching with filters Not every plasmid search will start...in the previous layout, the number of plasmids matching each filter is displayed in parentheses.  In this...insert, or use the other options to find plasmids matching your search terms with gene and guide RNA / shRNA...

...they had even established their own labs.  Fried chicken with a side of inspiration  I look back at the ...randomized coffee trials for us at ASCB|EMBO 2019, matching Mentors with Singletons for short coffee breaks... even more valuable program to Microbe 2020 in Chicago.  Finding our place in networking and mentoring...Conference, and the Society for Advancement of Chicanos/Hispanics and Native Americans in Science’s (SACNAS...Joe taught high school chemistry for a year. The teaching/mentoring bug never went away. While he has frequent...

...problem! We also have chimeric versions of Anti-PSD-95 [K28/43R] in rabbit, chicken, and rat. Find Anti-...Tracking cellular history with protein ticker tape Teaching an old antibody new tricks   New compact, efficient... into the TurboID enzyme, Lee and Cheah et al. achieved precise control over when and where the enzyme... bioRxiv — doi: 10.1101/2021.10.13.463862.    Teaching an old antibody new tricks By Ashley Waldron While...