... for at least 4 hours.   If your final RNA sample(s) are resuspended in water or TE buffer, store them...break down RNA upon cell lysis.  In the late 1970’s, Chirgwin and colleagues showed that a strong protein...extraction. Researchers at the NIH in the mid 1980’s set out to develop a protocol that skipped the ultracentrifugation...

...References Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S. A modular cloning system for standardized...transcribed together. They can express one or more gene(s), and typically begin with a promoter and end with...

...Natl Acad Sci U S A. 1991;88(20):8972-6. Moks T, Abrahrnsen L, Oesterlof B, Josephson S, Oestling M, Enfors...polyhistidine tag, alternative fusion tags were used, such as S. aureus’ Protein A (Moks 1987). These tags, however...

..., C., Gao, H., Ru, G., Guo, X., Zhang, D., Zhang, S., Li, C., Wu, J., Randolph, P. B., Sousa, A. A., Qu... Wei, Y., Zhou, Y., Li, Y., Wu, D., Jin, M., Cui, S., Li, H., Li, G., Zhou, J., Yuan, Y., Zhang, H., Shi...Yang, H. (2024). Development of deaminase-free T-to-S base editor and C-to-G base editor by engineered human... Hou, X., Wang, H., Wei, Z., Liu, X., Li, Y., Li, S., Liu, Y., Zhang, X., & Bi, C. (2024). Glycosylase-based...

....  Mahlandt, E. K., Palacios Martínez, S., Arts, J. J., Tol, S., van Buul, J. D., & Goedhart, J. (2023...single-component systems take advantage of BcLOV4’s light-induced binding of the plasma membrane. In previous...doi.org/10.1016/j.ceb.2015.08.005.  Tei, R., Bagde, S. R., Fromme, J. C., & Baskin, J. M. (2023). Activity-based...

...technology and how It works 18:02 - 19:56 : Why Sample 6's first test was for Lysteria 19:56 - 24:41 : Who is.... Koeris' Website, biotechstart.org Read Sample 6's Paper on Bacteriophage-based Diagnostics Additional...

...experiments: Retrieve Ensembl Transcript ID(s) of your favourite gene(s) from Ensembl genome browser: https:/...index.html Design crRNA/ssODN: Using Ensembl Transcript ID(s) as an input query, pick crRNA (guide RNA sequence...Garcia-Diaz C, Blin C, Grant V, Bulstrode H, Gogolok S et al. 2017. Efficient CRISPR/Cas9-assisted gene targeting...PubMed PMID: 9928493. 4. Liang X, Potter J, Kumar S, Ravinder N, Chesnut JD. 2017. Enhanced CRISPR/Cas9...

...Trinklein ND, Thie H, Wilkinson IC, Tandon AK, Anderson S, Bladen CL, Jones B, Aldred SF, Bestagno M, Burrone... XY, Eng H-Y, Lane D, Lefranc M-P, Clark M, Dübel S (2018) When monoclonal antibodies are not monospecific...Publications, New York. Helma J, Cardoso MC, Muyldermans S, Leonhardt H (2015) Nanobodies and recombinant binders...

...the protein-coding capacity of a locus. When using S. pyogenes Cas9 (SpCas9), potential target sites are...10.1038/s41587-020-0561-9 Dempster JM, Pacini C, Pantel S, Behan FM, Green T, Krill-Burger J, Beaver CM, Younger... Ding Q, Collins R, Ragavendran A, Brand H, Erdin S, Cowan CA, Talkowski ME, Musunuru K (2014) Low Incidence...10.1016/j.stem.2014.04.020 Yang L, Guell M, Byrne S, Yang JL, De Los Angeles A, Mali P, Aach J, Kim-Kiselak...

...plant cells. Like other model systems, expression of S. pyogenes Cas9 or Cas9 variants (hereafter referred.... References Belhaj K, Chaparro-Garcia A, Kamoun S, Nekrasov V (2013) Plant genome editing made easy:...https://doi.org/10.1128/mmbr.67.1.16-37.2003 Khatodia S, Bhatotia K, Passricha N, Khurana SMP, Tuteja N (2016...7: . https://doi.org/10.3389/fpls.2016.00506 Kwak S-Y, Lew TTS, Sweeney CJ, Koman VB, Wong MH, Bohmert-Tatarev...

...yeast inducible CRISPRi library - Ampl133 targets S. cerevisiae genes with 6-12 guides per gene where ...new anti-CRISPRs were identified that inhibit the S. aureus Cas9, a Cas protein that is smaller and easier...pre-synaptic targeted synaptophysin tagged eGFP. AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE from Hongkui Zeng'...

..., Nik Yusof, N. K., Lee, C. K., Abu Bakar, S., AbuBakar, S., Hollox, E. J., & Boon Peng, H. (2015). A ...2792.1. Epub 2011 May 9. PMID: 21554069. Haridan, U. S., Mokhtar, U., Machado, L. R., Abdul Aziz, A. T., ...

...References Bondeson, D.P., Mullin-Bernstein, Z., Oliver, S. et al. Systematic profiling of conditional degron...Nabet B, Roberts JM, Buckley DL, Paulk J, Dastjerdi S, et al. The dTAG system for immediate and target-specific...proteins in mammalian cells. Proc Natl Acad Sci U S A. 2012 Dec 4;109(49):E3350-7. doi: 10.1073/pnas.1216880109...

... E Root, James McFarland, Rameen Beroukhim, Jesse S Boehm. bioRxiv 2022.11.18.517155; doi: https://doi.org...-04601-8 Lewis, Anna CF, Santiago J. Molina, Paul S. Appelbaum, Bege Dauda, Anna Di Rienzo, Agustin Fuentes...252. Carlson J, Henn BM, Al-Hindi DR, Ramachandran S. Counter the weaponization of genetics research by...

...resolution by spatiotemporal expression profiling. Martin S et al., Mol Cell Proteomics. 2010 Dec;9(12):2654-65...

...'s Biosafety Resource Guide . References Cohen S, Au S, Panté N. How viruses access the nucleus. Biochim...

...deposited plasmids for the expression of cathepsin S, L, and B. This became a great starting point for ...Bertozzi CR, Carette JE, Stanley SA, Harris E, Konermann S, Hsu PD (2021) Genome-wide, bidirectional CRISPR screens...Garcia N, Whiteman MB, McKay LGA, Lelis FJN, Habibi S, Cai Y, Rennick LJ, Duprex WP, McCarthy KR, Lavine...

...codon bias across all endogenous genes in E. coli and S. cerevisiae (Tuller et. al., 2010). Figure ...doi.org/10.1038/nsmb1031 Gustafsson C, Govindarajan S, Minshull J (2004) Codon bias and heterologous protein...synthetic biology in Natural or synthetic? How Addgene;s dataset reveals trends in biological innovation Resources...

... because I only left him/her with small tasks and s/he was always asking for more work (this is a big ...your network…. I found it helpful to plan my week(s) in advance as much as possible so I could more easily...support you, just like you are supporting your report(s). This means that you should be able to go to your...

... Hao, Y. A., Lee, S., Roth, R. H., Natale, S., Gomez, L., Taxidis, J., O'Neill, P. S., Villette, V., Bradley...