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  1. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...References Bondeson, D.P., Mullin-Bernstein, Z., Oliver, S. et al. Systematic profiling of conditional degron...Nabet B, Roberts JM, Buckley DL, Paulk J, Dastjerdi S, et al. The dTAG system for immediate and target-specific...proteins in mammalian cells. Proc Natl Acad Sci U S A. 2012 Dec 4;109(49):E3350-7. doi: 10.1073/pnas.1216880109...

  2. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ... E Root, James McFarland, Rameen Beroukhim, Jesse S Boehm. bioRxiv 2022.11.18.517155; doi: https://doi.org...-04601-8 Lewis, Anna CF, Santiago J. Molina, Paul S. Appelbaum, Bege Dauda, Anna Di Rienzo, Agustin Fuentes...252. Carlson J, Henn BM, Al-Hindi DR, Ramachandran S. Counter the weaponization of genetics research by...

  3. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ..., Nik Yusof, N. K., Lee, C. K., Abu Bakar, S., AbuBakar, S., Hollox, E. J., & Boon Peng, H. (2015). A ...2792.1. Epub 2011 May 9. PMID: 21554069. Haridan, U. S., Mokhtar, U., Machado, L. R., Abdul Aziz, A. T., ...

  4. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...190500 Homer1L/S scFv [L113/130] L113/130 scFv 2a Homer1L/S Mouse Mouse 190501 Homer1L/S scFv [L113/130...Gamma2L/S scFv [N452/73] N452/73 scFv 2T GABA(A)R, Gamma2L/S Human Mouse 190556 GABA(A)R, Gamma2L/S scFv ... Human Mouse IgG2a 177456 Anti-Homer1L/S [L113/130R] Homer1L/S Mouse Mouse IgG2a 177457 Anti-Homer1L [...IgG2a 177553 Anti-GABA(A)R, Gamma2L/S [N452/73R] GABA(A)R, Gamma2L/S Human Mouse IgG2a 177554 Anti-GABA...N476/9R] ZIP3 Mouse Mouse IgG2a 206600 Anti-S-tag [N56/9R] S-tag Bovine Mouse IgG2a 206601 Anti-TrpC4 [...Human Mouse IgG2b 206636 Anti-Homer1L/S [L113/130R-2b] Homer1L/S Mouse Mouse IgG2b 206637 Anti-Homer1L... Human Mouse IgG1 206687 Anti-Homer1L/S [L113/130R-1] Homer1L/S Mouse Mouse IgG2a 206688 Anti-Homer1L ...

  6. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ...deposited plasmids for the expression of cathepsin S, L, and B. This became a great starting point for ...Bertozzi CR, Carette JE, Stanley SA, Harris E, Konermann S, Hsu PD (2021) Genome-wide, bidirectional CRISPR screens...Garcia N, Whiteman MB, McKay LGA, Lelis FJN, Habibi S, Cai Y, Rennick LJ, Duprex WP, McCarthy KR, Lavine...

  7. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...codon bias across all endogenous genes in E. coli and S. cerevisiae (Tuller et. al., 2010). Figure ...doi.org/10.1038/nsmb1031 Gustafsson C, Govindarajan S, Minshull J (2004) Codon bias and heterologous protein...synthetic biology in Natural or synthetic? How Addgene;s dataset reveals trends in biological innovation Resources...

  8. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ... because I only left him/her with small tasks and s/he was always asking for more work (this is a big ...your network…. I found it helpful to plan my week(s) in advance as much as possible so I could more easily...support you, just like you are supporting your report(s). This means that you should be able to go to your...

  9. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ... heterologous systems (e.g. mammalian proteins in S. cerevisiae). While this may be difficult to resolve...1985; 43:729-736. PubMed PMID: 3907859. 4. Fields, S., Song, O. A novel genetic system to detect protein-protein...membrane protein interactions. Proc Natl Acad Sci U S A. 2004; 101(33):12242–12247. Pubmed PMID: 15299147...

  10. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ... and resources References Aschauer DF, Kreuz S, Rumpel S (2013) Analysis of Transduction Efficiency, Tropism... OS, Velardo MJ, Peden CS, Williams P, Zolotukhin S, Reier PJ, Mandel RJ, Muzyczka N (2004) Recombinant....org/10.1016/j.ymthe.2004.05.024 Dudek AM, Pillay S, Puschnik AS, Nagamine CM, Cheng F, Qiu J, Carette...

  11. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...012198382-6/50026-1 Wang R, Xiang S, Feng Y, Srinivas S, Zhang Y, Lin M, Wang S (2013) Engineering production...doi.org/10.3389/fimmu.2017.01589 Ayyar BV, Arora S, Ravi SS (2017) Optimizing antibody expression: The...

  12. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...processed miRNA or siRNAs then silence the target gene(s) either transcriptionally or post-transcriptionally...produced that contains sequence against your gene(s) of interest and the reporter. This approach greatly...Plant Biology 7:251–257 . https://doi.org/10.1055/s-2005-837597 Li J-F, Chung HS, Niu Y, Bush J, McCormack...F, Joberty G, Zinn N, Mueller WF, Clauder-Münster S, Eberhard D, Fälth Savitski M, Grandi P, Jakob P, ...

  13. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ...detectable using PCR. The system also functions in S. pneumoniae and can be used to generate multiple mutations...gene lambda Red. pTargetF contains the specific gRNA(s), and the repair template is supplied as a dsDNA fragment...Perutka J, Powell JE, Geng P, Richhart DD, Byrom M, Kar S, Davies BW, Ellington AD, Moran NA, Barrick JE (2018....7b00399 Li Q, Sun B, Chen J, Zhang Y, Jiang Y, Yang S (2021) A modified pCas/pTargetF system for CRISPR-...AK, Carette X, Potluri L-P, Sharp JD, Xu R, Prisic S, Husson RN (2016) Investigating essential gene function...

  14. Viral Vectors

    Type
    Collection
    ...'s Biosafety Resource Guide . References Cohen S, Au S, Panté N. How viruses access the nucleus. Biochim...

  15. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...showed rapid response (60 or 130 ms on and 0.7 or 2.5 s off, for mouse or human receptors, respectively). ...AAV vector inventory. References Darvesh, Altaf S., et al. "In vivo brain microdialysis: advances in...

  16. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...western blot and immunofluorescence. GST  Glutathione S-Transferase (GST) is a 26 kDa tag which can be isolated...doi.org/10.1002/0471140864 Fox, J. D., & Waugh, D. S. (2002). Maltose-Binding Protein as a Solubility Enhancer...

  18. Doudna Lab CRISPR Plasmids

    Type
    Collection
    ...editing in human cells. Jinek M, East A, Cheng A, Lin S, Ma E, Doudna JA. eLife . 2013 Jan 29;2:e00471. PubMed...

  19. Lentiviral Prep Service

    Type
    Collection
    ...Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance ...pBOB-CAG-SARS-CoV2-Spike-HA Expression of SARS-CoV-2 spike (S) protein Pao 154985 pWPI-IRES-Puro-Ak-ACE2 Expression...
Showing: 141 - 160 of 413 results