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  1. Lentiviral Guide

    Type
    Guide
    ...accomplished by PCR amplifying the insert sequence using primers that contain the restriction sites needed. Functional...a certain number of bases from the ends of the primers used. Alternatively, you could ligate a multiple...

  2. CRISPR Guide

    Type
    Collection
    ...flap. However, the longer pegRNA also encodes a primer binding site (PBS) and the desired edits on a RT...gRNA; a specialized gRNA containing an additional primer binding site and reverse transcription template...

  3. Cloning

    Type
    Guide
    ...Complementary overhangs are built into the PCR primers for the insert, based on the destination vector...

  4. Plan Your Experiment

    Type
    Guide
    ... region of interest can be PCR amplified using primers that (A) flank the region of interest (deletions...

  5. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...encodes a unique restriction enzyme site and unique primer sites for more rapid screening by restriction digest...

  7. CRISPR Guide

    Type
    Guide
    ...flap. However, the longer pegRNA also encodes a primer binding site (PBS) and the desired edits on a RT...gRNA; a specialized gRNA containing an additional primer binding site and reverse transcription template...

  8. CRISPR 101 eBook...en español

    Type
    Blog Post
    ...¡La primera edición de nuestro libro electrónico CRISPR 101 ya está disponible en español! Esta traducción...
Showing: 121 - 129 of 129 results