... into DNA. This also requires an exogenous DNA template. HDR, however, is a very low-efficiency process... is >30nt from the proximal ends of the repair template (Yang et al., 2013). This means that, for gene...the Director of R&D in the Genetic Perturbation Platform at the Broad Institute and has worked with many...

...Keisuke Kaji Jonathan Katz Nathan Lawson Kathrin Plath Miguel Ramalho-Santos Derrick Rossi Michael Ruppert...

...repaired by homologous recombination with a repair template.  Each zinc finger recognizes a nucleotide triplet...advertisement of the technology on various non-scientific platforms, CRISPR has also transcended into the world of... and reverse transcriptase. Using an elongated template + guide RNA called pegRNA (prime editing RNA),...

...Nickase mutants can also be used with a repair template to introduce specific edits via homology-directed...

...Esposito Lab for use with the Gateway® Cloning Platform (Life Technologies™) to permit construction of...

... Special Collections EXPRESs Plasmids Human Platelet EXPRESs Plasmids for Human Erythrocyte Receptors...

...possibly resulting in frameshifts. If a repair template with high homology to the DNA surrounding the ...

...charged as they pass through a metal deflection plate. Each droplet contains a single cell that is assessed...for a desired parameter is collected in a tube or plate, while a droplet containing a negative cell is discarded.... Many FACS sorters are compatible with 96-well plates as collection vessels and can sort single cells...

... in the collection have been developed using a platform (Link opens in a new window) based on yeast display...

...Jaramillo Lab Riboregulation Keasling Lab BglBrick Platform , Terpenoid Production , and Propionate Induction...

...binding site (PBS) and the desired edits on an RT template. During prime editing, the pegRNA directs the ...

...set of plasmids for building homologous repair templates that incorporate a self-excising drug selection...

...bind adenenine with lower specificity). With the template for studying this type of technology already laid...

...Associate Director of the Genetic Perturbation Platform at the Broad Institute, John Doench. A genetic...Associate Director of the Genetic Perturbation Platform at the Broad. His mom always told him that he ...

...asked the individuals behind some of these new platforms what their motivations were for developing these...research in their field including datasets and platforms for discussing new developments, and more. As ...

...by an inexpensive and widely available imaging platform: ultrasound. Challenges of in vivo imaging with...ultrasound imaging. Ultrasound is an attractive imaging platform for in vivo imaging experiments because  ultrasound...

...system with your homologous recombination (HR) template, you could then: Confirm your HR by doing Restriction...screen for positive ones.  -- OR -- If your HR template has a selection marker such as puromycin, you ...

...months. Ampicillin breaks down especially fast and plates should be used witin 1 month for optimal efficiency... them to media hotter than about 55oC and store plates/stocks wrapped in foil if a light-sensitive antibiotic...

...creatively to deliver a highly specific, programmable platform well-suited for various innovative biological ...enabling access to multiple plasmids used for various platform applications. For more information on CRISPR/Cas...

...directed repair templates (see the supplementary info from the paper for details on repair template design). ... Methods 2012. Mar 11;9(5):493-8. MXS cloning platform for imaging and flow cytometry applications Designing...relatively easy to design constructs and repair templates to insert TAP tag fusions directly into the genome...