...cells were transfected in 96-well format using an Amaxa Shuttle device; 5 days after the transfection, the...

...FRET acceptor. These experiments will define the maximum and minimum energy transfer values you can expect...

...duplex, and it’s the reason guide sequences have a maximum length of 20 bases: nothing longer will fit. Stabilization...

...probabilistically ON or OFF following Cre recombination to maximize label diversity Mammalian Megason 13769 pCALNL-DsRed...

... further optimization can be performed to both maximize the separation and reduce any background staining...

... a structured motif to enhance pegRNA stability PEmax - optimized PE architecture; increased synergy between.... PMID: 25079318 Bondy-Denomy, J., Pawluk, A., Maxwell, K. L., & Davidson, A. R. (2013). Bacteriophage...Rollins, M. F., Hidalgo-Reyes, Y., Wiedenheft, B., Maxwell, K. L., & Davidson, A. R. (2015). Multiple mechanisms...Kranzusch, P. J., Sontheimer, E. J., Davidson, A. R., Maxwell, K. L., & Doudna, J. A. (2017). A Broad-Spectrum..., J., Edraki, A., Shah, M., Sontheimer, E. J., Maxwell, K. L., & Davidson, A. R. (2016). Naturally occurring...

...that antibodies may need longer to reach their maximum binding, so feel free to trial even longer incubation...

...either case, next-generation sequencing of the maxiprep DNA is recommended to verify that the library ...process. Amplify the library (electroporation and maxiprep). If delivering as virus, make virus; this creates...

...your substance maximally absorbs. In the case of nucleic acid (DNA and RNA), the maximal absorbance is ...and most have a specific wavelength that they maximally absorb. By measuring the absorbance of a liquid...is at 260nm. Protein maximally absorbs at 280nm and the ratio of nucleic acid to protein (260/280) is generally...

...and on which you can individually distinguish a maximum of ~100 colonies Pro-Tip If you will be screening...like to make 20 plates, and our plates can hold a maximum of 10 mL, we’ll want 200 mL of media total. Importantly...

... it is also important to consider factors that maximize cleavage of the desired target sequence or on-target...

...primer should be a minimum of 50 nucleotides and a maximum of 300 nucleotides from your target. Are there ...

...fill the water bath, and be sure to know what the maximum fill capacity is. Look for a “fill” line inside...

... might hold a pipette. Without moving past the maximum possible setting on the pipette, set the desired...

...this protocol could, in theory, give rise to a maximum of 48 monoclonal cell lines. When this protocol...

...but do not stick to PES. Using a PES filter will maximize titer . DMEM high glucose, Corning 10-013-CV DMEM...

...receptor AIS, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM AREG amphiregulin AR, CRDGF, MGC13647, SDGF...

...with AgeI. Mix: 6 μg pLKO.1 TRC-cloning vector (maxiprep or miniprep DNA) 5 μL 10x NEB buffer 1 1 μL AgeI...