We narrowed to 324 results for: cloning

Check out all of the plasmids featured in our hot plasmids articles from 2017.

Use blue light to control gene expression from PBAD promoters. It's more spatially and temporally precise compared to AraC based expression vectors.

The Addgene blog has expanded tremendously in 2015. Check out our curated list of our top viewed posts from 2015!

This post, written by the creator of the CRISPRecise kit, explains how to use it to minimize off-target effects in CRISPR genome editing.

Review of off-target effects in CRISPR experiments. Topics covered include: measuring, preventing, and controlling for off-target events.

We've recently curated a Microbiology Plasmid Collection. Learn what you can find in the collection in this post.

Not getting enough DNA from your plasmid preps? This post outlines variables like endA and low copy number that could affect DNA yields and suggests steps to super-charge your plasmid preps.

Designing a CRISPR experiment in bacteira? Check out these tools for genome engineering, CRISPRa, CRISPRi, base editing, and microbiome engineering.

Welcome to Addgene's blog! In our blog we'll help you share science by highlighting the latest plasmid technologies, bringing you the inside scoop from scientists, providing career development advice, and more.

A review of the advantages of epitope tags and commonly used tags and their antibodies.

This post describes the design of shuttle vectors, which are designed for replication, selection, and expression in more than one host species.

Chromoproteins are a subset of the fluorescent protein family. They give strong color in visible light and can be used for molecular biology experiments.

Find out which of Addgene's blog posts were most popular in 2014. Also, learn how to become a guest blogger for Addgene.

An all-in-one CRISPR/Cas9 Multiplex Genome Engineering System for transfecting and editing cell lines.

Ready to let your tags do the work for you? Learn about self-labelling protein tags and how they can give you flexibility in your experimental approach!

Addgene and Seeding Labs recently awarded plasmids to two researchers in Ghana. Read about their work inside.

Learn more about E. coli strains used for protein overexpression.

Learn more about luciferases, a class of enzymes used in bioluminescence, and how they are commonly used in reporter plasmids.

In the final post of our Quick Guide to Working with Drosophila, learn how to make Drosophila transgenic lines.

Learn about how ClonTracer, a lentiviral library for lineage tracing, was used to test if cancer cells have resistance to drugs before or after treatment.