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We narrowed to 332 results for: cloning

Showing: 121 - 140 of 332 results

  1. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ...template, using Gibson Assembly or restriction enzyme cloning. Streptomyces bacteria are more recombinogenic ...inducer, in this case anhydro-tetracycline (aTc). By cloning in two tandem copies of the gRNA cassette, one ...and the gRNA are Tet-inducible, and a gRNA can be cloned easily into pRH2521 using BbsI. This system results...syringae. These vectors contain BsaI sites for easy cloning of a gRNA of interest. Cytidine and adenine base...

  2. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ... about a week. Taking advantage of Golden Gate cloning, the Haynes lab’s Protocols.io protocol describes...useful vectors compatible with Gibson and Saptrap cloning systems to generate AID* tagged CRISPR knock-in...

  4. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...single cell clones for in vitro CRISPR experiments. The likelihood that a single cell clone derived from...may not be worth the cost and labor when single clones are selected. Conversely, translational research...

  5. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...that takes advantage of DNA sequencing to turn mAb clones into expression constructs to be conveniently modified...accuracy while screening. We also compare different clones of the same target to see if the results are the...

  6. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...DNA left over for other applications, like PCR, cloning, transfection, or long-term storage. But what about...incomplete. Protein expression strains are not good for cloning Bacterial strains adapted for protein purification...

  7. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...website to verify plasmids or validating your new clones, try these tips to make the most of your time and... to help other labs speed up their plasmid and cloning verification steps and free up more time for using...

  9. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...that have standard cloning sites or gateway compatible cloning sites for easy cloning of your genes of ... and repair templates of choice though modular cloning steps. The Voytas lab plans to update the toolkit...

  11. Of Myc and Men

    Type
    Blog Post
    ... by developing generic strategies for detecting cloned genes (Munro and Pelham, 1984). The strategy they...peptide tagging to detect proteins expressed from cloned genes: deletion mapping functional domains of Drosophila...

  15. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ...If you’ve ever used Golden Gate Assembly for cloning, you might be familiar with the rules of thumb for...complexity allowable by a favored, fairly easy cloning technique (which may be of particular interest ...

  17. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...specific genomic locus, which can require multiple cloning steps (Fujita et al., 2013). CRISPR-mediated purification...can be targeted using a gRNA, which can easily be cloned into a gRNA expression plasmid. Experimental use...
Showing: 121 - 140 of 332 results