...to find antibodies currently available from our catalog that target epitope tags and other common cellular...ready-to-use recombinant monoclonal antibodies undergo application-specific validation and quality control by Addgene...Source Species Isotype Reactivity Recommended Applications PI Do you have suggestions for other antibodies...

...synthetic biology plasmids related to cell-to-cell communications and environmental sensing. Sensing and Signaling... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Gene/Insert Vector Type Promoter Tags PI Publication Do you have suggestions for other plasmids that...

...to find antibodies currently available from our catalog that target proteins popular in neuroscience research...ready-to-use recombinant monoclonal antibodies undergo application-specific validation and quality control by Addgene...Source Species Isotype Reactivity Recommended Applications PI Do you have suggestions for other antibodies...

... has a unique 24 bp barcode that simplifies identification of the transcription factor isoform in pooled...used for screening transcription factors for applications such as cellular programming or cancer drug ...barcoded with a 24 bp sequence, to simplify identification during screening. Library ID PI Description...

...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...Protocols gRNA Design Tools CRISPR Blog Posts Using catalytically inactive Cas9 (dCas9) fused to a fluorescent...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions for other plasmids that...

...Recombinant Antibody Description Isotype Recommended Applications Browse Epitope Tag Antibodies IPI has developed...Antibody Collection page to browse Addgene’s full catalog of antibodies that target epitope tags and other...Recombinant Antibody Description Isotype Recommended Applications Browse Antibody Plasmids The following plasmids...

...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...introduce a double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs ...NHEJ. Plasmid Gene/Insert Selectable Marker PI Publication Libraries Toxoplasma CRISPR Knockout Pooled Library...

... Tools Tetracycline Transactivators Affinity Purification Cell Ablation Monosynaptic Neuronal Tracing ...research community. The entire Penn Vector Core catalog has been transfered to Addgene and available for...above (By Function or By Serotype). Convert a Penn Catalog ID to an Addgene ID Return to top Viral Service...

...pX458 plasmid family. They have made several modifications to the the px458 plasmid to expand its usefulness...developmental studies. Key plasmid features and pX458 modifications: replacement of Cbh promoter with EF1alpha addition...Plasmids from Collection ID Plasmid Description PI Publications Kaufman ML, Goodson NB, Park KU, Schwanke M,...

...reducing the time to experiments by removing the amplification and extraction steps required when one receives...determined by spectrophotometer. Other methods of quantification may yield slightly different DNA concentrations...Cloning grade DNA is designed for use in cloning applications. Direct transfection into cells is not recommended...

...bacteria to aquatic animals to insects. Luciferase catalyzes the oxidation of a substrate, leading to the emission...advantages and disadvantages depending on the application. Firefly luciferase is considerably brighter ...luciferase-based plasmid systems for many different applications. Browse the Collection Highlights section to...respectively, for increased usefulness in in vivo applications. LuxSit-i : An artificial luciferase desined...

...Protocols & Resources Contents of Kit Original Publication Oligomerized pool engineering (OPEN): an 'open-source... in the Materials and Methods of your future publications. For your Materials and Methods section: "The...The plasmid kit used in this publication was a gift from Keith Joung (Addgene kit # 1000000013)" For your...

...vesicular stomatitis virus G) are packaged as replication-defective virus particles with the SARS-CoV-2...protein is inefficient for pseudotyping; several modifications may enhance infectious particle production, ...particular viral spike protein (MERS, CoV-1, CoV-2), modification, or variant. pBOB-CAG-SARS-CoV2-Spike-HA (Addgene...

...which is not required for the transcription or replication of the genome within infected cells, but is required... cells that lack the G protein, the virus can replicate but the newly formed viruses are unable to infect...starter cell via the EnvA receptor. RABV is able to replicate in the starter cell using the G-protein, which...

... by Category Cloning and Genomic Tools Browse plasmids related to cloning and genomic modification, including... , Light Signaling , Sigma Factors , Resource Allocator and Orthogonal Switches Other Addgene Tools and...

...generally introduced in the media. A new codon to be allocated to the new amino acid. Because there are no free...single plasmid, with multiple copies of the tRNA. Applications By making small changes in selected amino acids...tRNA. And the orthogonal tRNA has to bind to an unallocated codon. Therefore many controls must be used to...code expansion without buildup of prematurely truncated protein. Ryan Mehl 197656 BL21(DE3) ΔserC serC...

...compatible with multiple applications (different fluorophores, biotin, bead purification, blocking agents) ACP...formation in nonribosomal peptide biosynthesis. Catalytic role of the condensation domain. J Biol Chem. ...

...Plasmid Description 44246 pdCas9-humanized A catalytically inactive, human codon-optimized Cas9 expression...expression plasmid 44247 pdCas9::BFP-humanized A catalytically inactive, human codon-optimized Cas9-BFP fusion...codon-optimized Cas9 constructs 44249 pdCas9-bacteria A catalytically inactive bacterial Cas9 expression plasmid 44250...

...Multipurpose Toolkit enables targeted, specific modification of monocot and dicot genomes using genome engineering...engineering; validated in tomato, tobacco, M. truncatula , wheat, and barley - Daniel Voytas. A CRISPR...Taproot is a podcast to understand how scientific publications are created. Papers from the plant biology literature...

...polymerase chain reaction (PCR), cloning, isothermal amplification, reverse transcription of RNA into DNA, and ...for fixing damaged and degraded DNA prior to amplification, enzymes for cutting protein sequences to remove...transform into E. coli bacteria for expression and purification ( pET28a-LIC or other pET vectors are recommended...