...designing oligos for cloning my target sequence into a backbone that uses the human U6 promoter to drive expression...

...our Plasmid Protocols Find Your Perfect Plasmid Backbone ...

...Nature Reviews Microbiology. 2011 Jun; 9, 467-477. Bondy-Denomy J, Pawluk A, Maxwell KL, Davidson AR.  Nature...

...practicing and getting familiar with the process. Bonus points for being able to just look at a paragraph...

...fluorescent dyes for DNA-protein interaction studies? Kyubong Jo's lab generated fluorescent protein fusions ...

...using heat and/or chemicals to break some of the bonds keeping the protein folded, changing it from its...

...RNAs per gene. The lab modified the lentiviral backbone to encode the CD90.1 selection marker, Thy1.1,...

...pBLADE-mCherry: This construct, built from the pBAD33 backbone, contains BLADE, the PBAD promoter, and a mCherry...

...to a cell but cannot create new particles. As a bonus, this increases the carrying capacity of the viral...

... base pairs are held together with two hydrogen bonds not three as G-C pairs are. As a result, stretches...

...MCS)), but do not cut elsewhere on the plasmid Bonus: It is helpful to choose restriction enzymes that...

...important in disease. Empty Backbones This table is a collection of empty backbone plasmids into which you...with Nano-lanterns Collection Highlights Empty Backbones Expression Constructs Reporter Constructs Luciferase...regulatory elements on gene expression. Empty backbones for inserting regulatory elements before or after...Promoter Description PI 60226 AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Renilla EF1α ...

...GSG-P2A-GCaMP6f-WPRE-pA Syn GCaMP6f mRuby2 Constitutive 1 Bonhoeffer , Huebener , Rose 51083 AAV-EF1a-DIO-GCaMP6f-...GSG-P2A-GCaMP6f-WPRE-pA CAG GCaMP6f mRuby2 Cre dependent 1 Bonhoeffer , Huebener , Rose 83899 pAAV-mDlx-GCaMP6f-Fishell...GSG-P2A-GCaMP6s-WPRE-pA Syn GCaMP6s mRuby2 Constitutive 1 Bonhoeffer , Huebener , Rose 51082 AAV-EF1a-DIO-GCaMP6s-...GSG-P2A-GCaMP6s-WPRE-pA CAG GCaMP6s mRuby2 Cre dependent 1 Bonhoeffer , Huebener , Rose 68720 pAAV-hSyn1-Flex-mRuby2...GSG-P2A-GCaMP6s-WPRE-pA Syn GCaMP6s mRuby2 Cre dependent 1 Bonhoeffer , Huebener , Rose 100842 pAAV.CAG.Flex.GCaMP6s.WPRE.SV40...

...cell. When cellular mRNAs are digested with ribonucleases, ribosomes protect the segments of mRNA to which...

...attention and answer my key questions. As an added bonus, I also got the opportunity to present what I learned...

...the fluorescent protein collection Find empty backbones for fluorescent protein fusions Browse the fluorescent...

...domains The original iGluSnFR in the pMinDisplay backbone is fused to a specific peptide segment (PDGFR ...

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...acquire the data you need on the new instrumentation. Bonus reasons Visiting gives you access to possible new...

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