Skip to main content
Addgene
Showing: 121 - 140 of 254 results

  1. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...non-integrating (adenovirus and AAV) but can still express episomally long-term (AAV). If you perform a lipid-based...with: either retrovirus, lentivirus, adenovirus, or AAV. Then, your desired DNA to be integrated can be cloned...

  2. AAV Molecular Tools

    Type
    Collection
    ...Ready-to-use AAV available from Addgene's viral service encoding tet-off transactivators and tools for... Viral Vector Packaging Service AAV Molecular Tools Viral Vector Packaging Service: Molecular...Tetracycline Transactivators and Inducible Tools These AAV encode tetracycline-inducible tools/controls and ... Gradinaru Tools for Affinity Purification These AAV encode tools for affinity purification (which can...Heintz , Nectow , Schmidt Neurophysiology Tools These AAV encode tools that can be used for neurophysiology...Name Expression System Activity Serotype PI 51509 AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE Syn-driven...tdTomato 8 Scanziani Tools for Cell Ablation These AAV encode tools that can be used for cell ablation. ...

  3. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...viral service pAAV_hSyn1_nLightR AAV9 Biosensors Patriarchi New viral service pGP-AAV-syn-jGCaMP8m-WPRE... Data Hub What is a Virus (and a Viral Vector)?  AAV Variables that Matter...serotypes pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] AAV5, AAV8 Optogenetics Boyden New serotypes pAAV-CAG-...serotype pAAV-hSyn-GRAB_OT1.0 AAV1, AAV9 Biosensors Li New viral service with multiple serotypes pAAV-hSyn-GRAB_OTmut...Category Depositor  Notes pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE AAV1, AAV5 Optogenetics Yizhar New...with multiple serotypes pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE AAV1, AAV5 Optogenetics Yizhar New viral...service with multiple serotypes pAAV_hSyn-PdCO-EGFP-WPRE AAV1, AAV5 Optogenetics Yizhar New viral service...

  4. Zhang Lab CRISPR Page

    Type
    Collection
    ...Posts Jump to: SpCas9 Plasmids GeCKO SAM AAV - In vivo Delivery AAV - Cas9 Mouse CRISPR ( C lustered R egularly... lung adenocarcinoma. Delivery of a single AAV vector ( AAV-KPL ) in the lung generated loss-of-function...nature14299. Epub 2015 Apr 1. PubMed . Return to AAV In vivo plasmids AAV Plasmids with Cas9 Mouse CRISPR-Cas9 knockin...#60958) contains pAAV-U6::sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). This is a AAV plasmid for sgRNA...This plasmid is used as a control for AAV-KPL. #60226 - AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR... Lab SAM Cloning Protocol 321.5 KB Return to top AAV - In vivo Genome Editing The Zhang Lab has both single...Cas9 and sgRNA in vivo via adeno-associated virus (AAV). The single vector system uses a smaller Cas9 from...

  5. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...variants come in AAV packaged virus aliquots being deposited at Addgene. Other plasmids and pAAVs can be ordered...protein CAG: Generic strong promoter. Formulated as AAV-DJ for neuronal expression. CAG-FLEX: CAG promoter... Biosensors Browse All Fluorescent Proteins Find AAV Tools References Marvin, Jonathan S., et al. “An...

  6. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...You may also like: Viral Service: All AAV Viviana Gradinaru PHP AAV Serotypes Blog: Viral Vectors As part...We provide high quality AAV preps from select plasmids in the repository. Browse the University of Florida... Viral Vector Packaging Service AAV University of Florida Serotype Testing Panel for ...viral vectors undergo quality control, including AAV titration by ddPCR, in vitro and (when possible) ...transduction of the mouse retina by tyrosine-mutant AAV serotype vectors. Mol Ther . 2009 Mar;17(3):463-71...One . Jun 8;10(6). PMID: 26052939 Lu, et al. 2016. AAV-mediated transduction and targeting of retinal bipolar...Rodriguez-Estevez, et al. 2020. Transduction optimization of AAV vectors for human gene therapy of glaucoma and their...

  7. Optogenetics AAV Preps

    Type
    Collection
    ...Browse all Optogenetics AAV *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from...high quality AAV preps from select plasmids in the repository. Browse our optogenetics AAV collection, ...Packaging Service AAV Optogenetics Viral Vector Packaging Service: Optogenetics AAV Optogenetic tools ...20071 pACAGW-ChR2-Venus-AAV CAG ChR2 Venus Constitutive 1, 9 Svoboda 83898 pAAV-mDlx-ChR2-mCherry-Fishell... 28017 AAV-CAG-hChR2-H134R-tdTomato CAG ChR2/H134R tdTomato Constitutive rg* Svoboda 75470 pAAV-CAG-FLEXFRT-ChR2...dependent 9 Histed 22222 AAV-FLEX-Arch-GFP CAG Arch GFP Cre dependent 1, 9 Boyden 28305 pAAV-FLEX-ArchT-tdTomato...20949 pAAV-double floxed-eNpHR-EYFP-WPRE-pA EF1a eNpHR EYFP Cre dependent 9 Deisseroth 26966 AAV-Ef1a-...

  10. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...Wilson AV-9-PV2453 45185-AAV9 AAV-EF1a-BbTagBY Joshua Sanes AV-9-PV2454 45186-AAV9 AAV-EF1a-BbChT Joshua Sanes...18917-AAV1 AAV-FLEX-rev-ChR2-tdtomato Scott Sternson AV-1-20071P 20071-AAV1 pACAGW-ChR2-Venus-AAV Karel Svoboda...18917-AAV5 AAV-FLEX-rev-ChR2-tdtomato Scott Sternson AV-5-20071P 20071-AAV5 pACAGW-ChR2-Venus-AAV Karel Svoboda...18916P 18916-AAV9 AAV-FLEX-rev-ChR2(H134R)-mCherry Scott Sternson AV-9-18917P 18917-AAV9 AAV-FLEX-rev-ChR2... AV-5-PV2432 22222-AAV5 AAV-FLEX-Arch-GFP Ed Boyden AV-1-ALL864 51503-AAV1 AAV pCAG-FLEX-tdTomato-WPRE...ALL854 51502-AAV1 AAV pCAG-FLEX-EGFP-WPRE Hongkui Zeng AV-1-PV0102 105531-AAV1 pAAV.CMV.LacZ.bGH James M. ...51502-AAV2 AAV pCAG-FLEX-EGFP-WPRE Hongkui Zeng AV-2-PV0101 105530-AAV2 pAAV.CMV.PI.EGFP.WPRE.bGH James...

  11. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...improvement of optogenetic adeno-associated virus (AAV) vectors. Listed below are a few additional uses ...Since most optogenetic reporters are delivered using AAV injection, the majority of the cells surrounding ...about Other Means of Genome Engineering Using FLEx AAV Vectors? Learn about Viral Vector Elements Read Our...

  12. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ...details in this blog post. 9. Droplet Digital PCR for AAV Quantitation by Kate Harten DeMaio We made the switch... switch to droplet digital PCR to quantify AAV titers. Get the details on the methods in this blog post...

  13. Chemogenetics Plasmids

    Type
    Collection
    ...Sternson 119741 AAV SYN flex PSAM4 GlyR IRES EGFP PSAM4-GlyR Syn IRES-EGFP Yes Sternson 119742 AAV SYN PSAM4...Roth 70717 AAV-mOXT-hM3Dq-mCherry-WPRE hM3D (Gq) Mouse Oxycotin mCherry No Geschwind 66795 pAAV-PTRE-tight-hM3Dq-mCherry...Sternson 119744 AAV CAMKII PSAM4 GlyR IRES EGFP PSAM4-GlyR CamKII IRES-EGFP No Sternson 196041 pAAV Syn intron...Chemogenetics Guide Viral Service: Chemogenetics AAV Blog: Chemogenetics Viral Vectors 101 Blog: Which...Cre- or Flp- dependent. We also offer ready-to-use AAV preparations of select chemogenetic plasmids; these...Recombinase Cre-dependent Flp-dependent Plasmid type AAV Mammalian expression, non-viral Viral Service Available...2a-hM4Dnrxn hM4Dnrxn (Gi) CAG mCherry No Sternson 52521 AAV-CAG::FLEX-rev:: ChR2HA-2a-hM4D hM4Dnrxn (Gi) CAG ...

  14. Viral Vectors

    Type
    Collection
    ... Adeno-associated Virus (AAV) Plasmids | Guide Adeno-associated viruses (AAVs) are commonly used for gene...not permanently integrated into the host genome. AAVs are limited by their small packaging capacity. Adenovirus...research goals . For example, adeno-associated virus (AAV) is typically preferred for in-vivo studies because...Stable RNA <8 Kb 80-130 Dividing/Non-dividing Yes Low AAV Transient or Stable* single stranded linear DNA ∼...<8 Kb 80-130 Dividing Yes Moderate * Recombinant AAV has a low frequency of target cell genome integration...

  16. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...diseases. The researchers injected Cre-expressing AAV-5 into the DRN of a transgenic mouse harbouring a...Addgene has several recombinase-expressing ready-made AAV vectors which can be used in numerous floxed animal...viral service Find viral protocols and videos Find AAVs for your research ...vigilance. A recent study investigated the injection of AAV8 particles expressing an activating chemogentic receptor...specifically activate the Drd1 neurons, they injected AAV2 particles into a Drd1-Cre transgenic mouse to deliver...

  17. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    ...important characteristics of retroviruses, lentiviruses, AAVs, and adenoviruses in this easy-to-use guide. Print...

  18. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...are named after the viruses they’re derived from: AAVs, lentivirus, gamma retrovirus, and adenovirus. And... Vector Elements More resources from Addgene The AAV Data Hub Addgene's Viral Services Addgene's Viral...
Showing: 121 - 140 of 254 results