... following the rules outlined below. If you’re selected as a winner, you’ll receive a winter care package...both the classic lab and home editions. We will select up to 3 winners for each edition and the winners...

...negative data. We want to make sure people are selecting AAVs that will work for their experiments, and...viral prep. And if you've used the Data Hub to help select an AAV for your experiments, let us know (datahub...

...in the same place, making it easier to go from selection to viral vector. To start the AAV packaged on ...a banner below the ordering table. Click on it, select your desired serotype and volume, and submit your...

...can help researchers save time and money when selecting an antibody! We encourage everyone interested ...Hub Antibodies 101: Validation Antibodies 101: Selecting the Right Antibody...

...function. Using bioinformatic analyses, they also selected for other key properties, notably high solubility...antibody. From their initial pool of candidates, they selected 12 candidate protein domains from non-vertebrate...

...type. Find some transduction characteristics of select AAV serotypes in Table 2 from the paper. Delivery... given in Tables 3-7. 3. Making use of AAV’s selectivity for neurons: targeting defined neuronal populations...

...and Linux. Inkscape is versatile and has a wide selection of tools for drawing and editing shapes and like...to particular types of images. For example, by selecting “Tools” and then clicking the “Usage rights” dropdown...

...recent months: Our selection of GCaMP8 AAV preps has expanded. Check out the new selection of ready-to-use...

...fluorescent protein or something species-specific) or a selectable marker. Each insertion line can then be isolated...Transposons in the Lab Plasmids 101: Screens vs. Selections Plasmids 101: Knockout /Knock-In Plasmids ...

...were developed into 16 variants with different selection markers (blasticidin, neomycin, puromycin, hygromycin...fused to a region of the RPL41 gene, to drive the selection/reporter parts of each construct. This new tool...

...Curie in Paris, has developed the retention using selecting hooks (RUSH) system. How does it work? RUSH is...PEST2D sequences. Several pre-constructed promoter-selection marker or promoter-inducible expression related...proteins (FPs). A subpopulation of mitochondria is selectively labeled by irradiating the photoactivatable FP...PAGE and then staining the gel with DHFBI to selectively visualize the Broccoli-tagged RNA. This technique...

...of spending time and money producing virus from select vectors in the repository yourself, you can now...Cas9 nickase, Cas9 activators, Cas9 repressors, select gRNAs, and CRISPR pooled libraries. Whether you...

...have different epitopes available if it is being selected out of a complex mix of proteins that have been...condition or configuration.  Validation  Once you’ve selected an antibody, or multiple antibodies, you’ll need...

... the lentiviral backbone to encode the CD90.1 selection marker, Thy1.1, in addition to puromycin resistance...New GRAB-DA and 5HT sensors New AAV tools to selectively control parvalbumin interneurons from the Dimidschstein...

... genome) and the other contains the bacterial selection marker and origin of replication. During bacterial...saving a glycerol stock to ensure the colony you selected contains the full plasmid. A diagnostic digest...

...multiplexing. It is imperative that you use narrowly selective isotype-specific secondary antibodies when multiplexing...Antibodies 101: Polyclonal Antibodies Antibodies 101: Selecting the Right Antibody  ...

...sequence to be amplified (usually 18-21bp) When selecting restriction sites, you should use a DNA analysis... longer the expected PCR product is. You should select an annealing temperature based on the melting temperature...

...more selective for different ions. Right now, we can modulate electricity. If you could selectively let...

...markers into a target genome. They did this by selecting the important elements from existing transposon...  A new tool for CRISPR gRNA validation Selecting a gRNA sequence that effectively targets your ...sequence or gene of interest into the pCoofy vectors, select the appropriate pair of vector and gene primers...

...difficult for individual labs to make efficiently. For select viral vectors in the repository, you can instead...in the brain, mapping neuronal networks, and selectively activating signaling pathways. Many scientists...