...Antibody Validation Using the Indirect ELISA Method Run an indirect ELISA against a purified antigen to demonstrate...

...infection and selection G. Safety H. References H.1 Published articles H.2 Web resources I. Appendix I.1 Sequence...Biosafety Guide . Back to Top H. References H.1. Published Articles Functional siRNAs and miRNAs exhibit ...

... bacteria for a longer time, you will need to establish glycerol stocks. The addition of glycerol stabilizes...

...above result represents a single experiment. For publishable data, the experiment would need to be repeated...

... in other lanes contaminating your sample. To accomplish this, it is best to skip lanes between samples...

...restriction sites are present in a given sequence. For a list of the commonly used commercially available restriction...

...of T4 Pol in the following step. This may be accomplished by gel purification . Step 5: Create Insert ...

...community. Jane Hannon Our Marketing & Outreach Specialist Jane Hannon discusses her career path, current...

...speed and time (and temperature, if applicable) listed in your protocol. Pro-Tip Spin speed is often given...

...target a wide variety of recognition sequences. For a list of many commonly used restriction enzymes, visit...

...compatibly digested vector backbone. This is accomplished by covalently connecting the sugar backbone ...

...reproducibility and accelerates science. Here, we list the specific equipment, reagents, and methods that...

... linker (see Figure 1). The key to the biosensor lies in pH: DsRed is relatively pH-insensitive, while...plasmids in a future blog post. No time to read? Listen to our hot plasmids segment on the Addgene Podcast...constructed. The authors then used GMAP assembly to establish a collection of over 30 promoters and 140 genes...characterize positive hits. This work has been published in Cell. The Moffat laboratory maintains a website...Carlson RCIscript-GoldyTALEN, pC-GoldyTALEN Tom Ellis pTAL5-BB, pTAL6-BB David Grunwald pCS2TAL3-DD,...Center has kindly constructed and deposited an unpublished set of Zika gene plasmids to Addgene’s repository... prototrophy, can also aid in designing self-establishing metabolically cooperating (SeMeCo) communities...

...reproducibility and accelerates science. Here, we list the specific equipment, reagents, and methods that...

...Right image adapted by permission from Macmillan Publishers Ltd: Nature Protocols Grieger, Joshua C., Vivian...

...ends of the newly synthesized strands. Materials List Reagents for each 50µL PCR: Thin-walled PCR tube...

...deliver antibiotic resistance. This protocol was established using 293T cells but can be adapted to alternative...

...reproducibility and accelerates science. Here, we list the specific equipment, reagents, and methods that...

...reproducibility and accelerates science. Here, we list the specific equipment, reagents, and methods that...

...reproducibility and accelerates science. Here, we list the specific equipment, reagents, and methods that...